- Services Overview
- Analytes Details
- FAQ
What is Human Hematopoietic Soluble Receptor?
Human hematopoietic soluble receptors are key players in the regulation of hematopoiesis, the complex process by which all blood cells are generated from hematopoietic stem cells. These receptors belong to a broader category of proteins known as cytokine receptors, which mediate the actions of cytokines—small signaling molecules crucial for cell communication in immune responses, inflammation, and hematopoiesis.
Hematopoietic receptors typically exist in two forms: membrane-bound and soluble. Membrane-bound receptors are anchored to the cell surface, where they interact directly with cytokines to trigger intracellular signaling pathways. In contrast, soluble receptors are not attached to the cell membrane; they are present in the bloodstream and other bodily fluids, where they retain their ligand-binding capability. Soluble receptors arise through two primary mechanisms:
- Proteolytic Shedding: Membrane-bound receptors are cleaved by specific enzymes, releasing the extracellular domain into the circulation.
- Alternative Splicing: Some receptors are produced directly as soluble forms through alternative mRNA splicing, which excludes the transmembrane domain.
Human hematopoietic soluble receptors play a crucial role in regulating immune and inflammatory responses. Acting as decoys, these receptors bind to cytokines, preventing them from interacting with membrane-bound receptors, which dampens cytokine signaling and helps control excessive inflammation. Conversely, they can also stabilize cytokines in circulation, prolonging their activity and enhancing immune responses when needed, such as during infections or injury.
Given their regulatory roles, these soluble receptors are valuable biomarkers in research and clinical diagnostics. Abnormal levels are associated with various conditions, including autoimmune diseases, cancers, and hematologic disorders, making them important targets for monitoring disease progression and developing therapeutic strategies.
Human Hematopoietic Soluble Receptor Panel at Creative Proteomics
Creative Proteomics provides the Human Hematopoietic Soluble Receptor 15-plex Panel using an advanced magnetic bead-based Luminex multiplex assay. Luminex xMAP technology employs magnetic beads conjugated with specific antibodies to selectively capture target soluble receptors from biological samples. The multiplex assay format facilitates the simultaneous detection and quantification of multiple analytes, significantly improving the efficiency and comprehensiveness of the analysis. The integration of magnetic beads ensures high sensitivity and specificity, allowing for accurate measurement of soluble receptors, even at very low concentrations.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Human
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Human | Human Hematopoietic Soluble Receptor 15-plex Panel | C-Reactive Protein/CRP, CD30/TNFRSF8, CD40/TNFRSF5, CD163, CD25/IL-2R alpha, Chitinase 3-like 1, CXCL16, DPPIV/CD26, IL-6R alpha, MMP-9, Myeloperoxidase/PO, Resistin, TNF RI/TNFRSF1A, TRANCE/TNFSF11, RANK L, TREM-1 | Suitable for analyzing receptor profiles related to immune regulation, inflammation, cancer, and metabolic disorders. | +Inquiry |
Advantages of the Human Hematopoietic Soluble Receptor Luminex Assay
- Multiplexing Efficiency: The Luminex assay supports the simultaneous analysis of up to 15 soluble receptors within a single sample. This high-throughput capability enables extensive profiling of receptor interactions and signaling pathways, eliminating the need for multiple separate assays.
- Exceptional Sensitivity and Specificity: Thanks to the use of color-coded magnetic beads and specific antibodies, the Luminex assay offers exceptional sensitivity and specificity. It can detect receptor levels down to picograms per milliliter (pg/mL), crucial for identifying minor changes in receptor levels associated with different diseases.
- Broad and Inclusive Data Coverage: Covering a diverse range of 15 vital receptors, including growth factors, adhesion molecules, and signaling proteins, the panel provides an in-depth understanding of complex biological processes, such as cancer progression, inflammation, and vascular biology.
- Cost and Time Efficiency: Consolidating multiple receptor assays into a single test, the Luminex approach reduces sample usage, testing time, and costs. This methodology offers considerable savings when compared to performing 15 individual ELISA tests, making it a cost-effective option for large-scale studies and clinical diagnostics.
- Customizable and Flexible Panels: Creative Proteomics provides customizable panels, allowing researchers to select specific receptors or modify the panel according to their research needs. This flexibility ensures the assay can be tailored to fit specific hypotheses or study objectives precisely.
- Consistent and Reproducible Results: With intra-assay precision generally ranging from 5% to 10% and inter-assay CVs typically below 15%, this assay delivers consistent and reliable results. Its reproducibility ensures dependable performance across different experiments and time periods.
- Sensitivity for Low-Abundance Targets: The assay's advanced detection system permits the measurement of receptors at very low concentrations, which is crucial for detecting early biomarkers or slight changes in receptor expression, with detection thresholds as low as 1 pg/mL.
Sample Requirements for Human Hematopoietic Soluble Receptor Luminex Panel
| Sample Type | Volume Required | Storage Conditions | Notes |
|---|---|---|---|
| Serum | 50-100 µL | -80°C | Avoid repeated freeze-thaw cycles. |
| Plasma (EDTA or Heparin) | 50-100 µL | -80°C | Ensure plasma is free of hemolysis. |
| Cell Culture Supernatant | 100-200 µL | -80°C | Collect supernatant before cell lysis. |
| Cerebrospinal Fluid (CSF) | 50-100 µL | -80°C | Special precautions required for collection. |
| Urine | 100-200 µL | -80°C | Centrifuge to remove debris before freezing. |
Why Analyze Human Hematopoietic Soluble Receptors?
Understanding Immune Regulation: These receptors are key to immune system function, influencing how immune cells communicate and regulate responses. Studying them reveals how the immune system maintains balance and responds to various stimuli.
Exploring Inflammation: Soluble receptors play a central role in inflammation by affecting cytokine activity. Analyzing these receptors helps elucidate inflammatory pathways and mechanisms, which is important for understanding chronic inflammation.
Investigating Cellular Processes: These receptors are involved in cell adhesion, migration, and tissue remodeling. Researching their levels and functions provides insights into processes like tissue repair and cancer progression.
Supporting Drug Development: In drug discovery, understanding soluble receptors aids in identifying new therapeutic targets and assessing the effects of drug candidates, refining therapeutic strategies.
Advancing Basic Research: Studying soluble receptors drives innovation in immunology, cell biology, and biochemistry by uncovering new aspects of receptor biology and signaling pathways.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Targets Details |
|---|
| C-Reactive Protein/CRP: An acute-phase protein that rises in response to inflammation, widely used as a biomarker for inflammatory conditions. |
| CD30/TNFRSF8: A receptor involved in immune regulation, particularly in T-cell and B-cell interactions, with implications in Hodgkin's lymphoma. |
| CD40/TNFRSF5: A receptor crucial for immune response regulation and inflammation, playing a key role in B-cell activation. |
| CD163: A scavenger receptor on macrophages involved in the resolution of inflammation. |
| CD25/IL-2R alpha: The alpha chain of the IL-2 receptor, important in T-cell activation and regulation. |
| Chitinase 3-like 1: A protein associated with tissue remodeling and inflammation, with elevated levels in various cancers. |
| CXCL16: A chemokine involved in immune cell recruitment and inflammation. |
| DPPIV/CD26: An enzyme and receptor with roles in immune regulation and glucose metabolism. |
| IL-6R alpha: A receptor for interleukin-6, central to inflammatory and immune responses. |
| MMP-9: A matrix metalloproteinase involved in tissue remodeling and metastasis. |
| Myeloperoxidase/MPO: An enzyme in neutrophils that contributes to pathogen defense and inflammation. |
| Resistin: A hormone associated with inflammation and insulin resistance. |
| TNF RI/TNFRSF1A: A receptor for tumor necrosis factor-alpha, involved in systemic inflammation. |
| TRANCE/TNFSF11/RANK L: A cytokine essential for bone metabolism and immune system regulation. |
| TREM-1: A receptor amplifying inflammatory responses, particularly in acute and chronic inflammatory conditions. |
How does the Luminex xMAP technology used in this panel work?
The Luminex xMAP technology leverages color-coded magnetic beads coated with specific antibodies that capture target soluble receptors from your samples. Each bead set is unique to a particular receptor, allowing for the simultaneous measurement of multiple analytes in a single sample. Here's how it works:
- Bead Coating: Magnetic beads are coated with specific antibodies that bind to the receptors of interest.
- Sample Incubation: The biological sample is incubated with these beads, enabling the receptors to bind to the antibodies on the beads.
- Detection: A secondary detection reagent, typically a fluorescently labeled antibody, is added to bind to the captured receptors. The beads are then analyzed using a Luminex instrument, which uses fluorescence to quantify the amount of each receptor present.
What is the turnaround time for results?
The turnaround time for results typically ranges from 2 to 4 weeks, depending on the complexity of the analysis and the volume of samples. We strive to provide timely and accurate results while maintaining high standards of quality
How can I get started with the Human Hematopoietic Soluble Receptor Panel service?
To get started, please contact Creative Proteomics to discuss your research needs and objectives. We will guide you through the process, from sample collection and preparation to data analysis and interpretation. Our team is dedicated to providing comprehensive support to ensure your study's success.
