- Services Overview
- Analytes Details
- FAQ
Cytokine/Chemokine in Bovine
Cytokines and chemokines are small signaling proteins that regulate the immune system by coordinating immune responses to infections, inflammation, and injury. In bovine species, these molecules play a critical role in immune activation, cell differentiation, and tissue repair. They are essential in mediating immune responses to pathogens such as Mycoplasma bovis, Brucella abortus, and Escherichia coli, which are major causes of disease in cattle.
Chemokines are a subset of cytokines that specifically control the movement of immune cells to sites of infection, a process known as chemotaxis. This helps recruit immune cells like neutrophils, macrophages, and T cells to areas where they are needed. Understanding the profile of cytokines and chemokines in cattle is crucial for improving disease management, vaccination strategies, and overall herd health.
As the livestock industry faces challenges from disease outbreaks and environmental stressors, studying cytokine and chemokine responses in bovine species offers valuable insights into improving animal health and managing infections effectively.
Bovine Cytokine/Chemokine Panel at Creative Proteomics
At Creative Proteomics, we provide comprehensive services for the analysis of bovine cytokines and chemokines through advanced multiplexed assays. The Bovine Cytokine/Chemokine Panel is designed to simultaneously measure multiple cytokines and chemokines, providing a detailed profile of the bovine immune response. Our panel is optimized for a wide range of applications, including immunological studies, disease research, vaccine development, and clinical diagnostics.
We use the Luminex xMAP Technology to perform high-throughput, quantitative analysis of bovine cytokines and chemokines. This technology allows for the precise detection and quantification of a wide variety of immune modulators in a single sample, saving time and resources while providing highly accurate results.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Bovine
Analytes Detected
| Species | Specification | Protein Targets | Application | Price |
|---|---|---|---|---|
| Bovine | Bovine Cytokine/Chemokine 15-Plex Panel | IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-17A, IL-36RA (IL-1F5), IP-10 (CXCL10), MCP-1 (CCL2), MIP-1α (CCL3), MIP-1β (CCL4), TNFα, and VEGF-A | Suitable for studying immune response modulation, inflammation, cell signaling, and biomarker discovery in bovine health and disease research. | +Inquiry |
Sensitivity (Detection Limits)
- IFNγ: 5–10 pg/mL
- IL-1α: 3–7 pg/mL
- IL-1β: 2–6 pg/mL
- IL-4: 1–4 pg/mL
- IL-6: 10–15 pg/mL
- IL-8 (CXCL8): 12–18 pg/mL
- IL-10: 6–12 pg/mL
- IL-17A: 15–25 pg/mL
- IL-36RA (IL-1F5): 20–30 pg/mL
- IP-10 (CXCL10): 8–14 pg/mL
- MCP-1 (CCL2): 5–10 pg/mL
- MIP-1α (CCL3): 3–8 pg/mL
- MIP-1β (CCL4): 4–9 pg/mL
- TNFα: 7–12 pg/mL
- VEGF-A: 10–20 pg/mL
Advantages of Bovine Cytokine/Chemokine Luminex Assay
- High Throughput: Luminex technology allows for the simultaneous measurement of up to 100 cytokines, chemokines, or other biomarkers in a single sample. This high throughput capability enables the analysis of multiple parameters without the need for additional assays, saving time and resources.
- Precision and Sensitivity: Luminex assays are known for their ability to detect even trace amounts of cytokines and chemokines with high accuracy. This is especially important when dealing with small sample volumes or rare biomarkers.
- Multiplexing: The ability to run multiple assays simultaneously on the same sample ensures that comprehensive immune profiling can be completed in a single run. Researchers can explore interrelationships between various cytokines and chemokines, providing a more complete picture of immune responses.
- Flexibility: Our Luminex-based panel is adaptable, allowing users to customize the assays based on specific research needs. Whether you need a general cytokine profile or a specialized focus on a particular immune pathway, the Luminex platform can meet your requirements.
- Cost-Effective: By reducing the number of assays needed and optimizing the use of biological samples, Luminex technology offers a cost-effective solution without compromising on the quality and accuracy of the data.
Sample Requirements for Bovine Cytokine/Chemokine Luminex Panel
| Sample Type | Minimum Volume | Storage Condition | Notes |
|---|---|---|---|
| Whole Blood | 1-2 mL | Collect in EDTA or heparin tubes. | Preferably collected in the early morning to reduce cytokine fluctuations. |
| Serum | 1 mL | Store at -20°C or lower. | Avoid hemolysis to prevent interference with assays. |
| Plasma | 1 mL | Store at -20°C or lower. | Same as serum; centrifuge within 1 hour of collection. |
| Tissue Homogenate | 100-200 mg | Store at -80°C. | Process immediately post-dissection to prevent degradation of analytes. |
Application of Bovine Cytokine/Chemokine Panel
- Immunology: Understanding how bovine immune systems respond to infections, vaccination, and disease.
- Infectious Diseases: Monitoring immune responses in cattle infected with pathogens, such as Brucella, Mycoplasma, and Bovine Respiratory Disease Complex (BRDC).
- Vaccine Development: Assessing immune responses to new vaccines and adjuvants, as well as tracking the efficacy of immunization protocols.
- Inflammation Studies: Studying the inflammatory response in cattle suffering from diseases such as mastitis, arthritis, or gastrointestinal disorders.
- Animal Health: Enhancing overall health management practices by identifying biomarkers of disease or assessing recovery post-surgery or injury.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| IFNγ | A key cytokine involved in innate and adaptive immunity, critical for activating macrophages and enhancing antigen presentation in bovine immune responses. |
| IL-1α | A pro-inflammatory cytokine that mediates immune cell recruitment and inflammatory signaling during infections and tissue damage. |
| IL-1β | Plays a central role in the inflammatory response, driving the production of additional pro-inflammatory mediators in bovine health. |
| IL-4 | An anti-inflammatory cytokine that promotes humoral immunity and regulates allergic responses. |
| IL-6 | A multifunctional cytokine involved in acute-phase responses, inflammation, and immune cell differentiation. |
| IL-8 (CXCL8) | A chemokine that recruits neutrophils to sites of infection or injury, essential for effective immune defense. |
| IL-10 | An anti-inflammatory cytokine that suppresses excessive immune responses and maintains immune homeostasis. |
| IL-17A | A cytokine involved in promoting inflammation and host defense against bacterial and fungal infections. |
| IL-36RA (IL-1F5) | An antagonist of IL-36 signaling, modulating inflammatory responses and protecting tissues from excessive damage. |
| IP-10 (CXCL10) | A chemokine that attracts activated T cells and plays a role in antiviral responses and immune regulation. |
| MCP-1 (CCL2) | A chemokine involved in monocyte recruitment and activation, important in inflammatory and immune processes. |
| MIP-1α (CCL3) | A chemokine that attracts and activates macrophages and lymphocytes, contributing to immune response and inflammation. |
| MIP-1β (CCL4) | Functions similarly to MIP-1α, with a role in immune cell recruitment and inflammatory signaling. |
| TNFα | A major pro-inflammatory cytokine critical for immune defense and inflammation, mediating cell signaling and immune responses. |
| VEGF-A | A growth factor promoting angiogenesis and vascular repair, also linked to inflammatory responses and tissue regeneration in bovine health. |
Can this panel be customized to include additional analytes?
Yes, we offer customization of the bovine cytokine/chemokine panel to include additional biomarkers not part of the standard 15-plex panel. Customization involves feasibility assessment, optimization, and validation to ensure accurate detection and quantification of your selected targets.
Can this panel be used for longitudinal studies or comparisons between time points?
Absolutely. The high reproducibility of Luminex assays makes them well-suited for longitudinal studies. Our rigorous quality control processes ensure consistent results across different runs, enabling reliable comparisons between time points or treatment groups.
How is data analysis performed, and what deliverables are included?
Our service includes comprehensive data analysis performed using advanced bioinformatics tools. Deliverables include:
- Raw data in standard formats (e.g., Excel or CSV).
- Concentration values for each analyte, with standard curves for validation.
- Statistical analyses and optional graphical representations of the cytokine/chemokine profiles.
What happens if my samples do not meet the quality requirements?
If we identify quality issues with your samples (e.g., degradation or contamination), we will notify you immediately and discuss potential solutions. Depending on the situation, we may recommend resampling or additional preprocessing steps.
