Introduction to epigenetics signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of epigenetics signaling pathway.

   Epigenetics Signaling Pathway Detection ServiceEpigenetics refers to changes in gene expression levels based on non-gene sequence changes, including DNA methylation, histone modification, chromosome remodeling and non-coding RNA regulation, mainly through the process of gene transcription or translation The regulation and control of it affects its functions and characteristics.

Epigenetics is a branch of genetics that studies the heritable changes in gene expression when the nucleotide sequence of a gene does not change. There are many epigenetic phenomena, known as DNA methylation, genomic imprinting, maternal effects, gene silencing, nucleolar dominance, dormant transposons Activation and RNA editing (RNA editing), etc.

Changes in epigenetic gene expression do not depend on changes in gene sequences, but on chemical modifications of DNA and histones, including acetylation, methylation, phosphorylation, ubiquitination, ADP ribosylation, etc. Modification of the transcriptional activity of genes, the current concept of epigenetics also includes the category of non-coding RNA (ncRNA). The most common epigenetic change in tumorigenesis is the methylation of CpG islands in the promoter region of tumor suppressor genes. Methylation silencing related gene expression can affect related tumor signaling pathways. Epigenetics has become a new target for individualized treatment of tumors, and many epigenetic changes can also be used as sensitivity markers for chemotherapeutics. Epigenetic drugs have also been used in clinical treatment, such as DNA methyltransferase inhibitor Decitabine and histone deacetylase inhibitor Vorinostat (SAHA) have been used in the treatment of tumors. Currently, many targeted drugs targeting key molecules in epigenetic regulation are undergoing clinical trials.

Our detectable targets:

DF1BIDIAPstBIDSODDBad
DF2BaxPIDDMYD88Rac1TLR4
DF3RIP1TRAF-2FADDAPAF-1BID
METTL3IRF7Mda-5NFκBRIG-1TRAF3
METTL14IRF9MEKK1p38RIP1TRAF5
Histone-H3IRF5MEK3p38MAPKSH2TRAF6
POL IIIRS1MEK6p50SLP76TRAM
FTOIRS2MSK1p65Tak1TRIF

Technology platform:

We provide Luminex technology for epigenetics signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

The ability to adjust epigenetics is essential to reset cell state and plasticity. Studies have found that some of the epigenetic changes that cause cancer can be reversed by new therapies, which in some cases are less toxic than traditional chemotherapy. Epigenetic methods will not only change researchers' views on cancer, but also change the treatment of cancer. The new epigenetic treatment is not to kill cancer cells, but to transform diseased cells into normal cells or make them more sensitive to existing or new therapies.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of epigenetics signaling pathway detection:

Epigenetics Signaling Pathway Detection Service

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing epigenetics signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Carter, B., Zhao, K. The epigenetic basis of cellular heterogeneity. Nat Rev Genet, 2021, 22: 235–250.
  2. Decombe, S., Loll, F., et al. Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability. Epigenetics & Chromatin, 2021, 14(35).
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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