Cytokines are soluble messenger molecules of the immune system. They are indicators of innate and adaptive immunity and have multiple stimulatory and regulatory functions during the immune response. Individual cytokines indicate certain immune responses and can be used to access immune status during health and disease. Cytokine assays can be used to understand the contribution to disease, pathology, protection, immune activation or suppression, and to detect changes in cytokine levels under the influence of diseases such as heterogeneous diseases, idiopathic cystitis and degenerative joint diseases, thus facilitating basic research on cat disease mechanisms, drug development, etc.
Creative Proteomics has developed a feline cytokine assay panel based on Luminex xMAP technology with the ability to screen for multiple biomarkers of systemic disease. The multiplex assay enables the simultaneous and rapid measurement of multiple immunoreactive proteins in a small number of biological samples. We can accept feline serum and plasma samples for customized assays, enabling single to multiple biomarker solutions.
The table below shows the types of feline Luminex protein analysis. You can choose from a variety of specifications as needed.
|Feline||Cytokine||19-Plex Panel||F1t3 Ligand、GM-CSF、GRO/KC、IFNy、IL-12(p40)、IL-13、IL-18、IL-1β、IL-2、IL-4、IL-6、IL-8/CXCL8、MCP-1/CCL2、PDGF-BB、RANTES/CCL5、SCF、SDF-1/CXCL12、sFas、TNFa|
|Feline||Metabolic Hormone||9-Plex Panel||Amylin (total), Ghrelin(active)%, GIP (total), GLP-1 (total), Glucagon, Insulin, Leptin, Pancreatic Polypeptide, PYY (total)|
Magnetic bead-based Luminex multiplex assay
Advantages of Feline Luminex Multiplex Platform
- High throughput and high speed: The analysis fluorescence speed can reach up to 5000~15000 pcs/s. It can perform real-time, qualitative and quantitative analysis of many different target molecules in the same sample at the same time in less than 3h.
- High sensitivity: The detection range can reach 3~5 orders of magnitude, and the lowest detection concentration can reach 0.1 pg/mL.
- High specificity and accuracy: homogeneous immunoassay is used without washing, which reduces the error of experimental results caused by washing during operation. Flow laser technology is used in the assay to detect each microsphere, preventing interference from heteroantigens (antibodies) in the specimen.
- Small amount of specimens: The simultaneous detection of multiple indicators in the same reaction well significantly reduces the amount of specimens compared to other methods, making it suitable for the detection of rare specimens.