Integrin Signaling Pathway Luminex Multiplex Assay

Integrin cell signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of integrin cell signaling pathway.

Integrin, as a family of cell surface receptors, is a transmembrane glycoprotein widely distributed on the cell surface. It is a heterodimer composed of a and β subunits through non-covalent bonds, and is an important complication on the cell surface. Receptors with adhesion and signal transduction functions. By combining the extracellular domain with the extracellular matrix (cell-extra-cellular matrixECM), the intracellular domain and the signal transduction molecule, integrins mediate the two-way signal transduction between the inside and outside of the cell, and regulate the growth, differentiation, migration, and growth of cells. Migration, invasion, apoptosis, survival and proliferation, adhesion, differentiation and other aspects play an important role.

All integrins are composed of two branches of non-covalent heterodimers a and B, of which there are 16 a-branches and 8 β-branches. The structure of integrins on the cell membrane is mainly composed of a larger extracellular zone, a transmembrane zone and a smaller intracellular zone. Different integrins have different ligands. Like other cell surface receptors, integrins with different affinities have different affinities for their ligands, and intracellular signals also affect the affinity of integrins and their ligands.

Integrins exist in two configurations: latent (low affinity state) and activated (high affinity state). Integrins combine with cytoskeleton signal transduction molecules and other proteins through the extracellular domain and the intracellular segment of ECM, and mediate the two-way signal transmission between the inside and outside of the cell. On the one hand, intracellular signals are transmitted through integrins, which activate them to regulate the affinity of integrins and extracellular ligands; on the other hand, the combination of integrins and ligands transmits extracellular signals into the cell, leading to cytoskeleton reorganization , Gene expression and cell differentiation, etc.

Our detectable targets:

Technology platform

We provide Luminex technology for integrin cell signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Each kind of microspheres is covalently cross-linked. Capture antibodies against specific antigens.

Integrin cell signaling plays an important role in regulating cell growth, differentiation, migration and migration, invasion, apoptosis, survival and proliferation, adhesion, differentiation, etc.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of integrin cell signaling pathway detection:

• High flexibility: specific probes, antigens or antibodies can be connected to the microspheres to meet the needs of different customers.
• High sensitivity: the detection limit can reach 0.01pg/ml.
• Good repeatability: similar homogeneous reaction mode, each indicator has 1000-5000 reaction units, and the median value of 100 analysis is taken.

Application of our service:

• To study the effects of drugs or therapies on integrin cell signaling pathways
• To study the effect of each virus on integrin cell signaling pathway
• To study the regulation mechanism of integrin cell signal pathway in disease

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing integrin cell signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

1. M Kabir-Salmani, S Shiokawa, et al. The role of alpha(5)beta(1)-integrin in the IGF-I-induced migration of extravillous trophoblast cells during the process of implantation. Mol Hum Reprod. 2020, 10(2): 91-97.
2. A van der Flier, A Sonnenberg. Function and interactions of integrins. Cell Tissue Res. 2019, 305(3): 285-298.