Introduction to apoptosis signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of apoptosis signaling pathway.

Introduction to apoptosis signaling pathwayApoptosis is the process of programmed cell death characterized by distinct morphological characteristics and energy-dependent biochemical mechanisms. Apoptosis is considered a vital component of processes including normal cell turnover, proper development and functioning of the immune system, hormone-dependent atrophy, embryonic development and chemical-induced cell death.

Apoptotic cells undergo morphological changes involving extensive plasma membrane blebbing followed by karyorrhexis. Apoptotic bodies are formed by separation of cell fragments during a process called "budding". Apoptotic bodies consist of cytoplasm with tightly packed organelles with or without a nuclear fragment. The organelle integrity remains enclosed within an intact plasma membrane. These bodies are subsequently phagocytosed by macrophages, parenchymal cells, or neoplastic cells and degraded within phagolysosomes. There are three main types of biochemical changes observed in apoptosis:

The two most widely characterised apoptotic pathways are the Intrinsic and Extrinsic pathways.

Our detectable targets:

p53BIDIAPstBIDSODDBad
BAKBaxPIDDMYD88Rac1TLR4
Bcl-xlRIP1TRAF-2FADDAPAF-1BID
FADDIRF7Mda-5NFκBRIG-1TRAF3
GASIRF9MEKK1p38RIP1TRAF5
Histone-H3IRF5MEK3p38MAPKSH2TRAF6
IKK-αIRS1MEK6p50SLP76TRAM
IKK-βIRS2MSK1p65Tak1TRIF
IPS-1ISGF3MSK2PI3KTBK1Vav
IRAK1ISREmTORPKRTLR3

Technology platform:

We provide Luminex technology for apoptosis signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

Apoptosis signal pathways mainly include endogenous and exogenous signal pathways. This pathway is widely used in cell renewal, normal development and function of the immune system, hormone-dependent atrophy, embryonic development and chemically induced cell death.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of apoptosis signaling pathway detection:

Advantages of apoptosis signaling pathway detection

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing apoptosis signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Ouyang, L. et al. Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell Proliferation, 2012, 45, 487-498.
  2. Ichim, G., Tait, S. W. G. A fate worse than death: apoptosis as an oncogenic process. Nature Reviews Cancer, 2016, 16: 539-548.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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