Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of ubiquitin proteasome signaling pathway.
Protein degradation mediated by the ubiquitin proteasome pathway is an important mechanism for the body to regulate the level and function of intracellular proteins. The components responsible for performing this regulatory process include ubiquitin and its initiating enzyme system and proteasome system. The ubiquitin priming enzyme system is responsible for activating ubiquitin and binding it to the protein to be degraded to form a polyubiquitin chain of the target protein, that is, ubiquitination. The proteasome system can recognize and degrade ubiquitinated proteins. In addition, there is another type of deubiquitinated protease that dissociates the ubiquitin chain molecule in the cell to form a reverse regulation.
The ubiquitin-proteasome pathway (upp) is composed of ubiquitinub and a series of related enzymes. In addition to ubiquitin, it also includes 4 enzyme families: ubiquitin-activating enzyme (E1), ubiquitin coupling enzyme (ubiquitin-conjugatingenzymesE2s) is also called ubiquitin carrier protein (ubiquitin-carrierprotein), ubiquitin-protein ligase (ubiquitin-ligatingenzymes (E3s) and proteasome (proteasome). Both protein ubiquitination and deubiquitination need to be mediated by multiple enzymes. Upp has both a high degree of substrate diversity and diversity for different regulatory mechanisms.
Ubiquitin-proteasome-mediated protein degradation pathways mainly include:
We provide Luminex technology for ubiquitin proteasome signaling pathway analysis.
Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.
Protein degradation mediated by the ubiquitin proteasome pathway is an important mechanism for the body to regulate the level and function of intracellular proteins. The proteasome pathway plays an important role in plant growth regulation, animal reproduction and development, tumorigenesis and neurological diseases.
In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.
Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing ubiquitin proteasome signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.