Introduction to ubiquitin proteasome signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of ubiquitin proteasome signaling pathway.

   Ubiquitin Proteasome Signaling Pathway Detection ServiceProtein degradation mediated by the ubiquitin proteasome pathway is an important mechanism for the body to regulate the level and function of intracellular proteins. The components responsible for performing this regulatory process include ubiquitin and its initiating enzyme system and proteasome system. The ubiquitin priming enzyme system is responsible for activating ubiquitin and binding it to the protein to be degraded to form a polyubiquitin chain of the target protein, that is, ubiquitination. The proteasome system can recognize and degrade ubiquitinated proteins. In addition, there is another type of deubiquitinated protease that dissociates the ubiquitin chain molecule in the cell to form a reverse regulation.

The ubiquitin-proteasome pathway (upp) is composed of ubiquitinub and a series of related enzymes. In addition to ubiquitin, it also includes 4 enzyme families: ubiquitin-activating enzyme (E1), ubiquitin coupling enzyme (ubiquitin-conjugatingenzymesE2s) is also called ubiquitin carrier protein (ubiquitin-carrierprotein), ubiquitin-protein ligase (ubiquitin-ligatingenzymes (E3s) and proteasome (proteasome). Both protein ubiquitination and deubiquitination need to be mediated by multiple enzymes. Upp has both a high degree of substrate diversity and diversity for different regulatory mechanisms.

Ubiquitin-proteasome-mediated protein degradation pathways mainly include:

Our detectable targets:

E1BIDIAPsTLR3SODDBad
E2BaxPIDDMYD88Rac1TLR4
E3RIP1TRAF-2FADDAPAF-1BID
FADDIRF7Mda-5NFκBRIG-1TRAF3
GASIRF9MEKK1p38RIP1TRAF5
IKK-αIRS1MEK6p50SLP76TRAM
IKK-βIRS2MSK1p65Tak1TRIF
IPS-1ISGF3MSK2PI3KTBK1Vav
IRAK1ISREmTORPKRTLR3

Technology platform:

We provide Luminex technology for ubiquitin proteasome signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

Protein degradation mediated by the ubiquitin proteasome pathway is an important mechanism for the body to regulate the level and function of intracellular proteins. The proteasome pathway plays an important role in plant growth regulation, animal reproduction and development, tumorigenesis and neurological diseases.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of Luminex technology:

Ubiquitin Proteasome Signaling Pathway Detection Service

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing ubiquitin proteasome signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Luciana C. Furtado, Anelize Bauermeister, et al. Isolated From the Brazilian Endemic Tunicate Euherdmania sp. Produces Dihydroeponemycin and Analogs With Potent Antiglioma Activity, Frontiers in Marine Science, 2021.
  2. Chee Wai Fhu, Azhar Ali, Dysregulation of the Ubiquitin Proteasome System in Human Malignancies: A Window for Therapeutic Intervention, Cancers, 2021.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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