Diabetes signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of diabetes signaling pathway.

Diabetes signaling pathway detection service(Joshi, T., et al., 2019)As the most serious microvascular complication, diabetes mellitus, caused by chronic hyperglycemia, leads to the dysfunction of many cells of the kidney and eventually to the development of renal failure.

The main pathophysiological alteration of diabetes signaling is insulin resistance in target tissues with insufficient insulin secretion. Among them, impaired insulin signaling plays an important role in the pathogenesis. Therefore, insulin signaling in diabetes is of great importance.

In diabetes, insulin binds to its receptor and causes intracellular signaling through the action of a series of intracellular signaling molecules, which activates two signaling pathways and finally reaches the effectors to produce various physiological effects. Diabetic signaling plays an important role in the physiological effects of insulin. Impaired insulin signaling can weaken the physiological effects of insulin, leading to insulin resistance and affecting diabetic signaling to induce diabetes.

The diabetic signaling system is regulated at multiple levels by different mechanisms and can be cross-linked with the signaling systems of other hormones (growth factors, cytokines) to modulate each other, thus ensuring that the diabetic insulin effect proceeds in an orderly manner according to physiological needs. Abnormalities in different aspects of diabetic signaling can interfere with the physiological function of insulin, leading to insulin resistance.

Diabetes affects a large number of people in the world. Lifestyle, obesity, dietary habits and genetic factors all contribute to this metabolic disease. The target pathway for controlling diabetes is the adenosine 5'-phosphate-activated protein kinase (AMPK) signaling pathway. AMPK affects diabetes signaling, and its activation enhances cellular uptake of glucose and inhibits intracellular glucose production.

Our detectable targets:

PTPc-MycULK1PFKPHGDHErk1
ShcLKB1LDHAANTVDACBax
Grb2PDHKLC3IPROLC3ULKFIP200
PI3KVPS34UYRAGNFκBRIG-1

TRAF3

CAPISGF3MSK2mTORTBK1Vav
CrkIIIRF9AMBRA1p38RIP1TRAF5
Gab-1IRS2MSK1p65Tak1TLR3
Shp-2IRF5MEK3p38MAPKSH2TRAF6
IRSI-4IRS1MEK6p50p53TRAM
TRIFIRAK1ISREPKR  

Technology platform:

We provide Luminex technology for diabetes signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.

In addition to Luminex multiplex assay, enzyme-linked immunosorbent assay (ELISA), flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of diabetes signaling pathway detection:

Advantages of diabetes signaling pathway detection

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing diabetes signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Joshi, T., et al. "Targeting AMPK signaling pathway by natural products for treatment of diabetes mellitus and its complications." Journal of Cellular Physiology, 2019.
  2. Zhonghua Qi, Yinghui Xu, et al. Baicalein alters PI3K/Akt/GSK3p signaling pathway inrats with diabetes-associated cognitive deficits. lnt J Clin Exp Med, 2018, 8(2): 1993-2000.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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