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Introduction

Creative Proteomics can simultaneously quantitatively detect immunomodulatory cytokines and proteins through a variety of technologies, and provide higher sensitivity and a wider detection range.

Immunomodulatory Cytokines Assay

Introduction of immunomodulatory cytokines

Immune modulatory cytokines are lymphokines synthesized by TH in regulatory T cells under antigen stimulation. These cytokines in turn participate in the regulation of TH, cell differentiation and function maintenance. IFN-γ and IL-4 are important factors that regulate TH cell differentiation and TH1/TH2 balance. Other cytokines that have been clearly involved in regulation include IL-2, IL-10 and IL-12, etc.

IFN-γ and IL-2 are mainly produced by TH1 cells, promote the differentiation of TH0 into TH1, and enhance the activity of TH1 cells. IFN-γ can effectively inhibit the differentiation of TH0 into TH2 and the proliferation of TH2 cells, but this effect is easily inhibited by IL-4. Factors that induce TH1 cell response are TGF-β and IL-12. IL-12 can enhance the proliferation of T cells induced by lectin and phorbol ester, and fully induce T cells and NK cells to produce IFNγ, forming a positive feedback of TH1 activation.

The main cytokines that induce TH0 to differentiate into TH2 cells are IL-4 and IL-10. IL-4 is an autocrine growth factor for TH2 cells. TH2 can form self-activated positive feedback through its secretion, and inhibit TH1 from producing IFN-γ through IL-4 and IL-10, and inhibit the differentiation and proliferation of TH1. IL-4 can promote CD40 ligand to activate B cells and produce TH2 type antibody IgG1. IL-10 can inhibit the production of IL-1, IFN-γ and GMCSF in TH1 cells to different degrees, but the most important thing is the inhibitory effect on IFN-γ at the mRNA level.

Detectable cytokines include but are not limited to:

IFN-αIFN-γL4IL-6IL-10
IL-12IL-13IL-23TGF-B14TNF-a

Technology platform

We mainly provide the Luminex cytokine detection platform. Luminex uses fluorescently encoded microspheres with specific antibodies to different target molecules. The different microspheres can be combined freely to a certain extent so that up to 100 analytes can be tested multiple times simultaneously in a single experiment.

The Luminex cytokine assay platform has the following advantages:

For your different needs, we can also provide the following detection methods:

Sample preparation

Creative Proteomics can provide you with a one-stop solution. If you want to test other cytokines or other information you want to consult, please contact us. We are looking forward to cooperating with you.

Reference:

  1. Jones G W, Hill D G, Cardus A, et al. IL‐27: a double agent in the IL‐6 family. Clinical & Experimental Immunology, 2018, 193(1): 37-46.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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