Immunomodulatory Cytokines Assay

Introduction

Creative Proteomics can simultaneously quantitatively detect immunomodulatory cytokines and proteins through a variety of technologies, and provide higher sensitivity and a wider detection range.

Introduction of immunomodulatory cytokines

Immune modulatory cytokines are lymphokines synthesized by T_H in regulatory T cells under antigen stimulation. These cytokines in turn participate in the regulation of T_H, cell differentiation and function maintenance. IFN-γ and IL-4 are important factors that regulate T_H cell differentiation and T_H1/T_H2 balance. Other cytokines that have been clearly involved in regulation include IL-2, IL-10 and IL-12, etc.

IFN-γ and IL-2 are mainly produced by T_H1 cells, promote the differentiation of T_H0 into T_H1, and enhance the activity of T_H1 cells. IFN-γ can effectively inhibit the differentiation of T_H0 into T_H2 and the proliferation of T_H2 cells, but this effect is easily inhibited by IL-4. Factors that induce T_H1 cell response are TGF-β and IL-12. IL-12 can enhance the proliferation of T cells induced by lectin and phorbol ester, and fully induce T cells and NK cells to produce IFNγ, forming a positive feedback of T_H1 activation.

The main cytokines that induce T_H0 to differentiate into T_H2 cells are IL-4 and IL-10. IL-4 is an autocrine growth factor for T_H2 cells. T_H2 can form self-activated positive feedback through its secretion, and inhibit T_H1 from producing IFN-γ through IL-4 and IL-10, and inhibit the differentiation and proliferation of T_H1. IL-4 can promote CD40 ligand to activate B cells and produce T_H2 type antibody IgG1. IL-10 can inhibit the production of IL-1, IFN-γ and GMCSF in T_H1 cells to different degrees, but the most important thing is the inhibitory effect on IFN-γ at the mRNA level.

Detectable cytokines include but are not limited to:

Technology platform

We mainly provide the Luminex cytokine detection platform. Luminex uses fluorescently encoded microspheres with specific antibodies to different target molecules. The different microspheres can be combined freely to a certain extent so that up to 100 analytes can be tested multiple times simultaneously in a single experiment.

The Luminex cytokine assay platform has the following advantages:

• Multiple detection: simultaneous detection of 100 biological targets
• Short experiment time: 1-3 weeks
• High sensitivity: the lower limit of accurate quantification is as low as 0.1 pg/mL
• Save samples: only need a sample volume as low as 25 μL
• Time saving: the experiment process only takes 4 hours

For your different needs, we can also provide the following detection methods:

• Flow cytometry (FACS analysis): Identify and measure cell biomarkers in complex subpopulations. It can be used for intracellular cytokine detection without two hours.
• Enzyme-linked immunosorbent assay (ELISA): Use the primary antibody for capture, and conjugate the secondary antibody with an enzyme or radioisotope for detection. Our multiplexing system can detect the expression of multiple cytokines at once.

Sample preparation

• Suitable for serum, plasma, cell culture supernatant and all kinds of body fluids
• Cells (whole blood cells, PBMC, mouse immune cells)
• The body fluid samples are stored in a refrigerator at -80 degrees Celsius and transported on dry ice

Creative Proteomics can provide you with a one-stop solution. If you want to test other cytokines or other information you want to consult, please contact us. We are looking forward to cooperating with you.

Reference:

1. Jones G W, Hill D G, Cardus A, et al. IL‐27: a double agent in the IL‐6 family. Clinical & Experimental Immunology, 2018, 193(1): 37-46.