Creative Proteomics can simultaneously quantitatively detect immunomodulatory cytokines and proteins through a variety of technologies, and provide higher sensitivity and a wider detection range.
Immune modulatory cytokines are lymphokines synthesized by TH in regulatory T cells under antigen stimulation. These cytokines in turn participate in the regulation of TH, cell differentiation and function maintenance. IFN-γ and IL-4 are important factors that regulate TH cell differentiation and TH1/TH2 balance. Other cytokines that have been clearly involved in regulation include IL-2, IL-10 and IL-12, etc.
IFN-γ and IL-2 are mainly produced by TH1 cells, promote the differentiation of TH0 into TH1, and enhance the activity of TH1 cells. IFN-γ can effectively inhibit the differentiation of TH0 into TH2 and the proliferation of TH2 cells, but this effect is easily inhibited by IL-4. Factors that induce TH1 cell response are TGF-β and IL-12. IL-12 can enhance the proliferation of T cells induced by lectin and phorbol ester, and fully induce T cells and NK cells to produce IFNγ, forming a positive feedback of TH1 activation.
The main cytokines that induce TH0 to differentiate into TH2 cells are IL-4 and IL-10. IL-4 is an autocrine growth factor for TH2 cells. TH2 can form self-activated positive feedback through its secretion, and inhibit TH1 from producing IFN-γ through IL-4 and IL-10, and inhibit the differentiation and proliferation of TH1. IL-4 can promote CD40 ligand to activate B cells and produce TH2 type antibody IgG1. IL-10 can inhibit the production of IL-1, IFN-γ and GMCSF in TH1 cells to different degrees, but the most important thing is the inhibitory effect on IFN-γ at the mRNA level.
We mainly provide the Luminex cytokine detection platform. Luminex uses fluorescently encoded microspheres with specific antibodies to different target molecules. The different microspheres can be combined freely to a certain extent so that up to 100 analytes can be tested multiple times simultaneously in a single experiment.
The Luminex cytokine assay platform has the following advantages:
For your different needs, we can also provide the following detection methods:
Creative Proteomics can provide you with a one-stop solution. If you want to test other cytokines or other information you want to consult, please contact us. We are looking forward to cooperating with you.