Creative Proteomics can simultaneously quantitatively detect immunomodulatory cytokines and proteins through a variety of technologies, and provide higher sensitivity and a wider detection range.
Immune modulatory cytokines are lymphokines synthesized by TH in regulatory T cells under antigen stimulation. These cytokines in turn participate in the regulation of TH, cell differentiation and function maintenance. IFN-γ and IL-4 are important factors that regulate TH cell differentiation and TH1/TH2 balance. Other cytokines that have been clearly involved in regulation include IL-2, IL-10 and IL-12, etc.
IFN-γ and IL-2 are mainly produced by TH1 cells, promote the differentiation of TH0 into TH1, and enhance the activity of TH1 cells. IFN-γ can effectively inhibit the differentiation of TH0 into TH2 and the proliferation of TH2 cells, but this effect is easily inhibited by IL-4. Factors that induce TH1 cell response are TGF-β and IL-12. IL-12 can enhance the proliferation of T cells induced by lectin and phorbol ester, and fully induce T cells and NK cells to produce IFNγ, forming a positive feedback of TH1 activation.
The main cytokines that induce TH0 to differentiate into TH2 cells are IL-4 and IL-10. IL-4 is an autocrine growth factor for TH2 cells. TH2 can form self-activated positive feedback through its secretion, and inhibit TH1 from producing IFN-γ through IL-4 and IL-10, and inhibit the differentiation and proliferation of TH1. IL-4 can promote CD40 ligand to activate B cells and produce TH2 type antibody IgG1. IL-10 can inhibit the production of IL-1, IFN-γ and GMCSF in TH1 cells to different degrees, but the most important thing is the inhibitory effect on IFN-γ at the mRNA level.
IFN-α | IFN-γ | L4 | IL-6 | IL-10 |
IL-12 | IL-13 | IL-23 | TGF-B14 | TNF-a |
We mainly provide the Luminex cytokine detection platform. Luminex uses fluorescently encoded microspheres with specific antibodies to different target molecules. The different microspheres can be combined freely to a certain extent so that up to 100 analytes can be tested multiple times simultaneously in a single experiment.
The Luminex cytokine assay platform has the following advantages:
For your different needs, we can also provide the following detection methods:
Creative Proteomics can provide you with a one-stop solution. If you want to test other cytokines or other information you want to consult, please contact us. We are looking forward to cooperating with you.
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