HGF signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of hepatocyte growth factor signaling pathway.

Hepatocyte Growth Factor (HGF) Adiponectin Signaling Pathway Detection Service(Fan S, et al., 2019)Hepatocyte growth factor (HGF) is a multifunctional cytokine originally described as a mesenchymal-derived factor that regulates cell growth, cell motility, morphogenesis, and angiogenesis by activating its receptor, a transmembrane tyrosine kinase encoded by the c-Met proto-oncogene. hGF and c-Met are frequently co-expressed or overexpressed in a variety of human malignancies, including glioblastoma tumors and medulloblastoma; and their expression levels correlate with poor prognosis.

HGF an endogenous tissue repair factor, attenuates apoptosis in many primitive cell types. the HGF signaling pathway eliminates Ang II-induced apoptosis by inhibiting cytochrome c release, caspase-3 activation, and DNA fragmentation. RNA immunoprecipitation experiments show that HGF stabilizes Bcl-xL mRNA by increasing nucleotide binding to the 3' untranslated region of Bcl-xL mRNA, which is associated with the cytoplasmic localization of nucleotides. hGF also blocked Ang II-induced caspase-3 activation and lactate dehydrogenase release in tissue explants in an ERK-dependent manner.

HGF and its receptor c-MET are commonly expressed in malignant gliomas and embryonic neuroectodermal tumors, including medulloblastomas, and appear to play an important role in the growth and propagation of these malignancies. Depending on the cellular context and the involvement of specific downstream effectors, HGF is shown to have both pro- and anti-apoptotic effects.

Our detectable targets:

RTKPI3KTYK2STAT1STAT2STAT3
PSGL1TLR3MAPKTRAF5MHC-IIRIP1
IRS2TLR4IRF3PKRicam1Mda-5
MSK1Rac1CREBmTORIRS1MEKK1
Tak1MYD88TRAF6ISREMEK6IRF9
TRIFJNKSH2Fasp50GAS
TNFRIRAK4p38MAPKp38SLP76TRAF3
ISGF3STAT5MEK3TLR9TRAMRIG-1
MSK2VavIRF5RelTCRNFκB
PI3KTBK1CD3NAP1  

Technology platform:

We provide Luminex technology for HGF signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.

Advantages of HGF signaling pathway detection:

Advantages of HGF signaling pathway detection

Application of our service:

Creative Proteomics has developed a signaling pathway target detection platform. We can provide detection services not only for HGF signaling pathways, but also for other signaling pathways. If you want to detect other targets, please contact us in advance and we will customize the service for you according to your requirements. Looking forward to working with you.

References:

  1. Fan S, Gao M, et al. Role of NF-kappaB signaling in hepatocyte growth factor/scatter factor-mediated cell protection. Oncogene, 2019, 24: 1749-1766.
  2. Rasola A, Anguissola S, et al. Hepatocyte growth factor sensitizes human ovarian carcinoma cell lines to paclitaxel and cisplatin. Cancer Res, 2018, 64: 1744-1750.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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