Interleukin (IL) -11 is an IL-6 family cytokine found in primate bone marrow stromal cells with a molecular weight of 23KD. IL-11 mediates downstream signal transduction by a common signal transduction molecule glycoprotein 130 (gp130). IL-11 is an anti-inflammatory factor that can inhibit inflammation by directly acting on macrophages and NK cells. IL-11 plays a promoting role in tumorigenesis and development. Upregulate the expression in various malignant diseases, and play a role in promoting cancer proliferation, tumor survival and metastasis by activating JAK / STAT3, RAS / ERK, PI3K / mTOR and other signaling pathways. Targeting IL-11, IL-11R and gp130 and other IL-11 Drugs that treat the target and its downstream signaling pathways (such as JAK / STAT3, RAS / ERK, and PI3K / mTOR) can provide new options for the treatment of IL-11-related tumors. For the reconstruction of bone marrow hematopoietic function after bone marrow transplantation and radiotherapy Sexual blood cell deficiency is of great value.
IL-11 is a cytokine produced by hematopoietic microenvironmental stromal cells with multiple functions. The human IL-11 gene encodes a precursor protein containing 199 amino acids. By removing the 21 amino acid sequence at the N-terminus, it becomes a monomer consisting of 178 amino acids. IL-11 includes three characteristics. First, it has no cysteine residue and no disulfide bond stable structure. Second, there are no sugar chain sites associated with aspartic acid. Third, the isoelectric point is 11.7. The IL-11 gene contains a 7 kb gene sequence, with 5 exons and 4 introns, located on chromosomes 19q13.3-19q13.4.
IL-11 exerts its biological function by binding to IL-11 receptor (IL-11R). As a high-affinity receptor, IL-11R is composed of ligand binding chain α chain and signal transduction chain gp130 (CD130), and belongs to the type I cytokine receptor family. JAK tyrosine kinase binds to gp130, causing phosphorylation of tyrosine residues at the distal membrane end of the cytoplasmic region of gp130, thereby binding STAT3 to gp130. Tyrosine kinase phosphorylates the tyrosine of STAT3 and leaves gp130 to form a homodimer. Subsequently, the dimeric STAT3 enters the nucleus for signaling.
IL-11 has effects on hematopoietic stem cells, megakaryocytes, lymphocytes and fat cells. IL-11 can enhance the differentiation of pluripotent stem cells under the growth factor, and promote the differentiation of B cells that produce immunoglobulin under T cell dependence. Besides, IL-11 can increase the production of proteins in the liver during acute inflammation, and inhibit the formation of fat cells. IL-11 used in vivo can effectively shorten the period of bone marrow suppression, accelerate the recovery of white blood cells and platelets. IL-11 cooperates with IL-3 and IL-4 to stimulate the proliferation of hematopoietic stem cells at rest, and affects the generation and differentiation of red blood cells. IL-11 can regulate the expression of plasma protein genes in hepatocytes and induce acute phase protein production.
Fig 1. Mechanism of Signaling
Creative Proteomics can provide cytokine detection platform for scientific research. According to different purposes, our dedicated analysts will customize exclusive solutions for you. We aim to provide customers with high-quality and convenient services to help you accelerate the progress of your project.
We mainly provide the Luminex cytokine detection platform. Luminex uses fluorescently encoded microspheres with specific antibodies to different target molecules. The different microspheres can be combined freely to a certain extent so that up to 100 analytes can be tested multiple times simultaneously in a single experiment.
The Luminex cytokine assay platform has the following advantages:
For your different needs, we can also provide the following detection methods:
For more information about the IL-11 detection service or need other detection requirements, please contact us.