Vaspin signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of vaspin signaling pathway.

Vaspin Signaling Pathway Detection Service(Hee J C, et al., 2019)Vaspin is a newly cytokine originally found in the visceral adipose tissue of Otsuka lobster de isle adipose rats. Vaspin is short for visceral adipose tissue-derived serine protease inhibitor. In addition to visceral adipose, vaspin is expressed in the skin, hypothalamus, pancreatic islets and stomach and has been shown to exert anti-inflammatory effects by inhibiting several pro-inflammatory adipokines such as leptin, resistin and tumor necrosis factor-alpha. Vaspin also stimulates adiponectin expression and improves insulin sensitivity in mice. expression of vaspin has been shown to worsen with diabetes and weight reduction with worsening diabetes and weight loss. Thus, administration of recombinant human vaspin improves glucose tolerance in diet-regulated mice, suggesting that it is a potential target for obesity-related diseases.

Vaspin has anti-atherogenic effects through Akt-mediated inhibition of endothelial cell apoptosis. Vaspin treatment significantly increased endothelial cell and isolated aortic NO secretion. Furthermore, vaspin treatment prevented fatty acid-induced reductions in endothelium-dependent vasodilation in isolated aorta of SD rats. Regarding the mechanism of vaspin-induced NO biosynthesis, vaspin activated the STAT3 signaling pathway and stimulated phosphorylation of eNOS through a STAT3-dependent mechanism (Ser 1177). In addition, vaspin treatment increased the expression of dimethylarginine dimethylaminohydrolase (DDAH) II, which is responsible for the degradation of ADMA, resulting in a decrease in ADMA levels. the vaspin-induced increase in DDAH II gene expression was stimulated by STAT3-mediated stimulation of DDAH II promoter activity. These suggest that vaspin decreases ADMA levels through STAT3-mediated regulation of DDAH II expression, thereby increasing eNOS activity.

Our detectable targets:

RTKPI3KTYK2STAT1STAT2STAT3
TNFRISGF3MSK2PI3KTBK1Vav
STAT5IRAK4JNKMYD88Rac1TLR4
CREBIRF3MAPKNAP1RelTLR9
MHC-IIIRS1MEK6p50SLP76TRAM
TCRicam1Mda-5NFκBRIG-1TRAF3
GASIRF9MEKK1p38RIP1TRAF5
PSGL1IRS2MSK1TLR3Tak1TRIF
CD3IRF5MEK3p38MAPKSH2TRAF6
FasISREmTORPKR  

Technology platform:

We provide Luminex technology for vaspin signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.

Advantages of vaspin signaling pathway detection:

Advantages of vaspin signaling pathway detection

Application of our service:

Creative Proteomics has developed a signaling pathway target detection platform. We can provide detection services not only for vaspin signaling pathways, but also for other signaling pathways. If you want to detect other targets, please contact us in advance and we will customize the service for you according to your requirements. Looking forward to working with you.

References:

  1. Hee J C, Je L W, et al. Vaspin Increases Nitric Oxide Bioavailability through the Reduction of Asymmetric Dimethylarginine in Vascular Endothelial Cells. Plos One, 2019, 7(12): e52346.
  2. KLOTING N, BERNDT J, et al. Vaspin genc expression in human adipose tissue : association withobesity and type 2 diabetes. iochem Biophys ResCommun. 2016, 339(1): 430-436.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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