Notch signaling pathway

Based on Luminex technology technology platform, Creative Proteomics provides analysis services for key targets of notch signaling pathway.

The Notch signaling pathway is a highly conserved cell signaling system present in most multicellular organisms. They regulate the development of many biological cells, from sea urchins to humans. Notch signal affects multiple processes of normal cell morphogenesis, including differentiation of pluripotent progenitor cells, apoptosis, cell proliferation, and formation of cell boundaries.

Notch signaling plays a pivotal role in the regulation of many fundamental cellular processes such as proliferation, stem cell maintenance and differentiation during embryonic and adult development. The notch cascade consists of notch and notch ligands, as well as intracellular proteins transmitting the notch signal to the cell's nucleus. In mammalian cells, there are four different notch receptors, referred to as NOTCH1, NOTCH2, NOTCH3, and NOTCH4, which display both redundant and unique functions. Notch receptors are large single pass Type I transmembrane proteins. The extracellular domain of all Notch proteins contains 29–36 tandem epidermal growth factor (EGF)-like repeats, some of which mediate interactions with ligand. After specific ligand binding, the intracellular part of the Notch receptor is cleaved off and translocates to the nucleus, where it binds to the transcription factor RBP-J. In the absence of activated Notch, RBP-J represses Notch target genes by recruiting a corepressor complex. Notch signaling is dysregulated in many cancers, and faulty notch signaling is implicated in many diseases including CADASIL (Cerebral Autosomal Dominant Arteriopathy with Sub-cortical Infarcts and Leukoencephalopathy), T-ALL (T-cell acute lymphoblastic leukemia), Alagille syndrome, MS (Multiple Sclerosis), and myriad other disease states.

Notch Signaling Pathway Detection Service

Our detectable targets:

TACEADAMNICDCrumbsNumbSOS
NAKa-adaptinFringeFurinS1O-Fut
HATCDK8SEL-10MAMMycHES
IL-4p21CtBPCSLSHARP

SMRT

SKIPRac1RhoMKK4MKK7p53
STAT3HDACC1REIK1STAT1NF-kB
S6Kc-MycSTAT2   

Technology platform

We provide Luminex technology for notch signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Each kind of microspheres is covalently cross-linked. Capture antibodies against specific antigens.

The Notch signaling pathway is a highly conserved cell signaling system that exists in most multicellular organisms. Notch signaling plays a key role in the regulation of many basic cellular processes, such as proliferation during embryonic and adult development, and stem cell maintenance and differentiation.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of notch signaling pathway detection:

Advantages of notch signaling pathway detection

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing notch signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Weng AP,Ferrando AA, et al. Activating mutations of Notch1 in human T cell acute lymphoblastic leukemia. Science, 2019, 306(5694): 269-271.
  2. Radtke F,Schweisguth F,et al.The Notch'gospel. EMBO Rep, 2020, 6(12): 1120-1125.
  3. De Strooper B, Annaert W, et al. A presenilin-1-dependent r-secretase-like protease mediates release of Notch intraCellular domain. Nature, 2019, 398(6727): 518-522.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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