Introduction to autophagy signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of autophagy signaling pathway.

Autophagy Signaling Pathway Detection ServiceAutophagy-related proteins (ATG) regulate this process in yeast and many ATG proteins are conserved in mammals. Individual ATG proteins and ATG-complexes support specific steps in the dynamic process of autophagy. The formation of a double membrane vesicle for the entrapment and delivery of cytosolic content to lysosomes is the hallmark of autophagy. Formation of the mature organelle or autolysosome involves a sequence of coordinated events. In each step, ATG proteins catalyze specific reactions critical for the maintenance of autophagic flux.

Autophagy has recently been shown to be an important component of the innate immune response. The signaling pathways leading to activation of autophagy in innate immunity are not well studied.Recent study shows that Toll-like receptor 4 (TLR 4) serves as an environmental sensor for autophagy. Defined a new molecular pathway in which lipopolysaccharide (LPS) induces autophagy in human and murine macrophages by a pathway regulated through Toll-interleukin 1 receptor domain-containing adaptor-inducing interferon-β (TRIF)-dependent, myeloid differentiation factor 88 (MyD88)-independent TLR4 signaling. Receptor-interacting protein (RIP1) and p38 mitogen-activated protein-kinase (MAPK) are downstream components of this pathway. This signaling pathway does not affect cell viability, indicating that it is distinct from an autophagic death signaling pathway. Further show that LPS-induced autophagy can enhance mycobacterial co-localization with the autophagosomes.

Regulatory pathways of autophagy signaling pathway:

Our detectable targets:

ATG13ATG2ATG12ATG5ATG7ATG10
ATG3ATG4JNKMYD88Rac1TLR4
ATG16WIPi2LC3IPROLC3ULKFIP200
VPS15VPS34UYRAGNFκBRIG-1TRAF3
GASIRF9AMBRA1p38RIP1TRAF5
Histone-H3IRF5MEK3p38MAPKSH2TRAF6
IKK-αIRS1MEK6p50SLP76TRAM
IKK-βIRS2MSK1p65Tak1TRIF
IPS-1ISGF3MSK2PI3KTBK1Vav
IRAK1ISREmTORPKRTLR3

Technology platform:

We provide Luminex technology for autophagy signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

The hallmark of autophagy is the formation of double-membrane vesicles, which are used to trap cytoplasmic content in the lysosome and deliver it to the lysosome. Autophagy signaling proteins play a vital role in maintaining autophagy flux, regulating innate immunity, and removing pathogens.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of autophagy signaling pathway detection:

Autophagy Signaling Pathway Detection Service

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing autophagy signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Ji-Won Lee, Hyeri Nam, et al. TLR4 (toll-like receptor 4) activation suppresses autophagy through inhibition of FOXO3 and impairs phagocytic capacity of microglia. Autophagy, 2019, 15(5): 753-770.
  2. Ming Chen, Jiaxing Liu, et al. Lipopolysaccharide mediates hepatic stellate cell activation by regulating autophagy and retinoic acid signaling. Autophagy, 2017, 13(11): 1813-1827.
  3. Romana T. Netea-Maier, et al. Modulation of inflammation by autophagy: Consequences for human disease. Autophagy, 2016, 12(2): 245-260.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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