Luminex Assay Services — Predefined & Custom Panels for Multiplex Detection

The Luminex xMAP® technology allows for the simultaneous detection of up to 100 analytes in a single 25–50 µL sample volume. Unlike ELISA, which requires a separate sample for every target, Luminex offers a broader dynamic range (4.5 logs) and significantly higher throughput. This makes it the gold standard for precious clinical samples and complex biomarker profiling, especially when sample volume is a limiting factor.

Our platform is highly versatile and validated for diverse biological matrices. Beyond standard serum and plasma (EDTA, Heparin, Citrate), we routinely process:

• Cell culture supernatants
• Tissue homogenates
• Cerebrospinal fluid (CSF)
• Synovial fluid
• Tears
• Bronchoalveolar lavage fluid (BALF)

Specialized extraction protocols are available for complex samples like fecal extracts or plant tissues.

To ensure data precision, every custom panel undergoes antibody interference and cross-reactivity validation. We perform recovery tests using recombinant proteins and linearity-of-dilution assays to verify that the multiplexed environment does not compromise the quantification of individual analytes. Our standard for intra-assay CV is typically <10%, ensuring high reproducibility across batches.

Yes. Our high-performance Luminex assays are optimized for sub-pg/mL sensitivity. By utilizing phycoerythrin (PE) as a fluorescent reporter and high-affinity antibody pairs, we can accurately quantify low-abundance cytokines and signaling proteins that often fall below the detection limits of conventional colorimetric assays.

Luminex often provides comparable or superior sensitivity, with detection limits reaching sub-picogram per milliliter levels. Its primary advantage is the ability to measure these low concentrations across a much wider linear dynamic range without the need for multiple sample dilutions.

Only 25 to 50 µL of sample is required to analyze all 45 targets simultaneously. This represents a 95% reduction in volume compared to running 45 individual ELISA tests.

Yes. We offer extensive panels for Mouse, Rat, Non-Human Primates (NHP), Canine, Feline, Porcine, and Bovine models, as well as specialized custom solutions for unique research species.

While both use xMAP technology, the FLEXMAP 3D® is a higher-throughput system capable of multiplexing up to 500 analytes per well with faster dual-laser scanning, whereas the Luminex 200™ is optimized for panels up to 100 analytes.