High‑Plex Protein & Biomarker Assays | High Sensitivity | Low Sample Volume | Flexible Customization
Creative Proteomics offers high-throughput Luminex Assay Services using the Luminex xMAP Technology for multiplex protein detection. Our services cater to immune profiling, biomarker discovery, and drug development, with both predefined multiplex panels and fully customized panel solutions designed to meet the specific needs of your research.
Simultaneously quantify up to 100 analytes (including cytokines, growth factors, and disease-specific protein targets) in a single well, significantly reducing experimental timelines.
Superior Sensitivity & Range
Detect low-abundance proteins with sub-pg/mL sensitivity and a broad dynamic range (4-5 logs), ensuring accurate measurement of both high and low concentration targets without multiple dilutions.
Maximized Sample Economy
Obtain a wealth of data from as little as 25–50 µL of sample—making it the ideal solution for precious or volume-limited clinical specimens.
Ready Panels + Flexible Customization
Optimized predefined panels + bespoke panel design across multiple species; kit options for in-house testing.
We offer a wide range of predefined panels for biomarker discovery, immunology, inflammation, oncology, and more. Our panels are designed to detect a variety of proteins and biomarkers in just one run, saving you time and increasing throughput.
Get the full list of our Predefined Luminex Assay Panels with detailed analytes, panel sizes, and species options to find the perfect fit for your research.
Custom Luminex Assay Panels — Tailored to Your Specific Needs
Start designing your custom multiplex assay today!
Share your analyte list, and our team will evaluate and design a panel tailored for your needs.
Expert Design & Optimization
We will select the appropriate bead and antibody combinations to ensure specificity and optimal performance.
Assay Execution & Data Analysis
Your assay will be performed in our laboratories, followed by comprehensive data analysis.
Final Report
You will receive a detailed report with raw data, standard curves, QC metrics, and data analysis.
Multiplex Assay Kits — Purchase and Use
For customers with Luminex instruments, we offer Multiplex Assay Kits for in-house protein target detection. Our kits are easy to use and come with everything needed for high-quality results.
Creative Proteomics provided custom multiplex assays for a client analyzing inflammatory biomarkers in Equine models. The project aimed to profile key cytokines such as FGF-2, G-CSF, GM-CSF, IL-6, IL-8, IL-17, KC/GRO, and MCP-1 across multiple samples to study immune response and disease mechanisms.
Challenges
The client needed to measure multiple cytokines (e.g., FGF-2, G-CSF, IL-6) from limited sample volumes (e.g., serum, plasma, or conditioned medium).
The research focused on inflammatory diseases such as osteoarthritis and respiratory conditions in horses, requiring accurate and high-sensitivity analysis of low-abundance biomarkers.
Our Solution
We developed a custom multiplex panel designed to simultaneously measure 8 cytokines in 25–50 µL of sample, ensuring high data density from limited volumes.
Using Luminex xMAP® technology, we provided high sensitivity (sub-pg/mL) and a broad dynamic range (4–5 logs) to capture both low and high-abundance analytes.
The final results were delivered in a publication-ready report, featuring standard curves and raw data to ensure data quality and interpretability.
Table 1. Data Results
FAQ
What is the primary advantage of Luminex multiplexing over traditional ELISA?
The Luminex xMAP® technology allows for the simultaneous detection of up to 100 analytes in a single 25–50 µL sample volume. Unlike ELISA, which requires a separate sample for every target, Luminex offers a broader dynamic range (4.5 logs) and significantly higher throughput. This makes it the gold standard for precious clinical samples and complex biomarker profiling, especially when sample volume is a limiting factor.
Which sample matrices are compatible with your Luminex services?
Our platform is highly versatile and validated for diverse biological matrices. Beyond standard serum and plasma (EDTA, Heparin, Citrate), we routinely process:
Cell culture supernatants
Tissue homogenates
Cerebrospinal fluid (CSF)
Synovial fluid
Tears
Bronchoalveolar lavage fluid (BALF)
Specialized extraction protocols are available for complex samples like fecal extracts or plant tissues.
How is the accuracy of custom multiplex panels guaranteed?
To ensure data precision, every custom panel undergoes antibody interference and cross-reactivity validation. We perform recovery tests using recombinant proteins and linearity-of-dilution assays to verify that the multiplexed environment does not compromise the quantification of individual analytes. Our standard for intra-assay CV is typically <10%, ensuring high reproducibility across batches.
Can this platform detect low-abundance biomarkers?
Yes. Our high-performance Luminex assays are optimized for sub-pg/mL sensitivity. By utilizing phycoerythrin (PE) as a fluorescent reporter and high-affinity antibody pairs, we can accurately quantify low-abundance cytokines and signaling proteins that often fall below the detection limits of conventional colorimetric assays.
Is Luminex more sensitive than ELISA?
Luminex often provides comparable or superior sensitivity, with detection limits reaching sub-picogram per milliliter levels. Its primary advantage is the ability to measure these low concentrations across a much wider linear dynamic range without the need for multiple sample dilutions.
How much sample is needed for a 45-plex protein assay?
Only 25 to 50 µL of sample is required to analyze all 45 targets simultaneously. This represents a 95% reduction in volume compared to running 45 individual ELISA tests.
Does Luminex technology support non-human species?
Yes. We offer extensive panels for Mouse, Rat, Non-Human Primates (NHP), Canine, Feline, Porcine, and Bovine models, as well as specialized custom solutions for unique research species.
What is the difference between Luminex 200 and FLEXMAP 3D?
While both use xMAP technology, the FLEXMAP 3D® is a higher-throughput system capable of multiplexing up to 500 analytes per well with faster dual-laser scanning, whereas the Luminex 200™ is optimized for panels up to 100 analytes.
