KLF4 signaling pathway
Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of KLF4 signaling pathway.
Kruppel-Like Factors (KLFs) are a family of transcription factors with zinc finger structure, which are involved in the regulation of several physiological activities and play a key role in apoptosis, differentiation, proliferation and embryonic development, and are closely related to eukaryotic gene transcriptional regulation.KLF4 is also known as gastrointestinal enriched Kruppel-like factor (GKLF) or epidermal zinc finger factor (EZF), Kruppel-like factor 4 (KLF4), as an important member of the KLFs family, shows high expression in primary hepatocellular carcinoma tissues, and KLF4 plays an important role in the biological function of primary hepatocellular carcinoma.
KLF4 protein is commonly expressed in the gastrointestinal epithelium, and the human KLF4 gene was cloned for the first time in 1998.KLF4 has a DNA-binding structural domain at the carboxyl terminus containing three C2H2 zinc finger structures, formed by 81 highly conserved amino acids, and has a high affinity for the GC box or CACCC element, which mainly recognizes and binds GC-rich DNA in the promoter region of target genes sequences. The amino terminus of KLF4 contains a highly variable transcriptional regulatory structural domain, containing a transcriptional activation structural domain between amino acid residues 91 and 117, rich in proline and serine, and a transcriptional repression structural domain between amino acid residues 181 and 388, rich in proline, indicating that KLF4 has two modes of activating and repressing gene transcription and plays different transcriptional regulatory function.
KLF4 can act as a putative tumor suppressor and transcription factor. The clinical significance, biological effects and mechanisms of KLF4 signaling in pancreatic cancer cells are highlighted by its important role in the effects of aerobic glycolysis in pancreatic tumors. Furthermore, the potential mechanisms of altered KLF4 expression and its effects on aerobic glycolysis in pancreatic cancer cells were found to be negatively correlated with KLF4 and LDHA expression in pancreatic cancer cells and tissues, and their expression correlated with the clinicopathological features of pancreatic cancer. Thus, dysregulated signaling in this novel KLF4/LDHA pathway significantly affects aerobic glycolysis and its development and progression in pancreatic cancer.
Our detectable targets:
We provide Luminex technology for KLF4 signaling pathway analysis.
Luminex technology is a multifunctional liquid phase analysis platform, mainly based on laser technology, colored microspheres, high-speed digital, and applied fluidics signal processing technology. Its core is the use of fluorescent dyes to encode polypropylene microspheres or magnetic microspheres. By adjusting the different mixing ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra are obtained. Perform a variety of hybridization reactions on microspheres with different fluorescent codes, such as antigen-antibody, enzyme-substrate, ligand-receptor binding reaction, and nucleic acid hybridization. Use laser detection to perform quantitative and qualitative analysis of microsphere encoding and reporter fluorescence respectively.
KLF4 is a zinc-finger transcription factor that is expressed in a variety of tissues including intestinal epithelium and skin and plays an important role in differentiation and cell cycle arrest. Depending on the gene targeted, KLF4 can activate and repress transcription. In addition, in certain cellular settings, KLF4 can act as an oncogene or tumor suppressor gene.
In addition to Luminex multiplex assay, enzyme-linked immunosorbent assay (ELISA), flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.
Advantages of KLF4 signaling pathway detection:
- High sensitivity: The microspheres have a large specific surface area, which can combine with the target molecules in the sample with the maximum ability, which improves the detection sensitivity. The lowest detection concentration can reach 0.1pg/mL.
- Strong specificity: It can automatically distinguish bound and unbound microspheres, and can quickly read the fluorescence signal on a single microsphere.
- High accuracy: Due to the large detection range of the Luminex detection platform, it does not need to be diluted like ELISA detection, and does not need to be washed. The fluorescence intensity of the reporter molecule on the microsphere is directly proportional to the binding molecule to be detected.
Application of our service:
- Study the regulatory mechanism of KLF4 signaling pathway in clinical medicine
- Research on the influence of various medical viruses on KLF4 signaling pathway
- Study the effects of carcinogenic drugs or carcinogenic therapies on KLF4 signaling pathway
Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing wee1 signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.
- Shi M, Cui J, et al. A Novel KLF4/LDHA Signaling Pathway Regulates Aerobic Glycolysis in and Progression of Pancreatic Cancer. Clinical Cancer Research, 2019, 20(16): 4370-4380.
- Ghaleb A M, Mcconnell B B, et al. Haploinsufficiency of Kruppel-Like Factor 4 Promotes Adenomatous Polyposis Coli–Dependent Intestinal Tumorigenesis. Cancer Research, 2020, 67(15): 7147.