Why Analyze Guinea Pig Cytokine?
Small signaling molecules known as cytokines are produced by a variety of immune system cells. They serve a crucial role in cell-to-cell communication by acting as immune response mediators and regulators. Interleukins (IL), interferons (IFN), tumor necrosis factors (TNF), and chemokines are only a few of the numerous groups of cytokines that can be grouped together. Numerous biological processes, including tissue repair, immune cell activation, and inflammation, are influenced by these substances.
Studying cytokines in guinea pig models is crucial for understanding the immune response and designing effective interventions in various diseases. Guinea pigs share many physiological and immunological similarities with humans, making them a valuable model for translational research. By analyzing the cytokine profile in guinea pigs, you can gain insights into the underlying mechanisms of diseases and evaluate the efficacy of potential therapeutics.
Guinea Pig Luminex Cytokine Panel Platform at Creative Proteomics
Luminex technology is a powerful multiplexed immunoassay technique that allows for the simultaneous detection and quantification of multiple analytes in a single sample. This technology combines the principles of flow cytometry, microspheres, and fluorescent detection. Each microsphere is internally dyed with different ratios of two fluorophores, allowing for identification of specific analytes by their spectral properties.
The Guinea Pig Luminex Cytokine Panel, developed by Creative Proteomics, is a cutting-edge tool designed specifically for guinea pig cytokine analysis. This panel provides a comprehensive analysis of multiple cytokines in guinea pig samples, enabling researchers to obtain a detailed cytokine profile in a single experiment. The panel consists of microspheres, each coated with a specific capture antibody that binds to its corresponding cytokine.
Detectable Guinea Pig Cytokine Assay Targets
| IL-1 | IL-2 | IL-4 | lL-6 | IL-10 | IL-11 |
| IL-12 | IL-13 | lL-15 | IL-16 | lL-17 | IL-18 |
| IL-21 | IL-22 | IL-23 | IL-27 | lL-33 | IFN-gamma |
| TNF-alpha | TNF-beta | TGF-beta | CCL2 | CXCL8 | CXCL10 |
| CCL5 | CCL20 | GM-CSF | MCP-1 | MIP-1 alpha | MIP-1 beta |
| Eotaxin | RANTES | VEGF | PDGF | FGF-basic | EGF |
| IGF-1 | BMP-2 | NGF | SCF | G-CSF | LIF |
Advantages of Our Guinea Pig Luminex Cytokine Panel
High Sensitivity: The panel demonstrates exceptional sensitivity, allowing for the detection of cytokines at low concentrations. For example, it can detect Interleukin-6 (IL-6) at levels as low as 0.5 pg/mL and Interleukin-10 (IL-10) at 1.0 pg/mL, enabling the precise quantification of even subtle changes in cytokine expression.
Broad Analytical Range: The panel covers a wide dynamic range, enabling the detection and quantification of cytokines across a broad concentration spectrum. It can accurately measure cytokines ranging from picogram to nanogram levels, accommodating the diverse expression levels of different cytokines in guinea pig samples.
Multiplexing Capability: The panel allows for the simultaneous measurement of multiple cytokines in a single sample, providing a comprehensive cytokine profile. It can detect and quantify up to 30 different cytokines in a single experiment, saving time and resources compared to traditional single-plex assays.
Accuracy and Precision: The Guinea Pig Luminex Cytokine Panel demonstrates excellent accuracy and precision in cytokine measurement. Its intra-assay and inter-assay coefficients of variation (CV) are consistently low, typically below 10%, ensuring reliable and reproducible results.
Time and Cost Efficiency: By enabling the measurement of multiple cytokines in a single experiment, the Guinea Pig Luminex Cytokine Panel significantly reduces the time and cost associated with traditional single-plex ELISA assays. Researchers can obtain a wealth of cytokine data from a small sample volume, optimizing resource utilization.
Comprehensive Data Analysis: The panel includes software that facilitates data analysis and interpretation, allowing researchers to extract meaningful insights from the cytokine profiles obtained. The software provides customizable data visualization and analysis tools, simplifying the interpretation of complex cytokine data sets.
Applications of the Guinea Pig Luminex Cytokine Panel
The Guinea Pig Luminex Cytokine Panel has found wide application in various fields of guinea pig research, including:
Infectious Disease Research: The panel enables the evaluation of cytokine responses during viral, bacterial, and parasitic infections in guinea pigs. This information aids in understanding the host immune response and identifying potential targets for therapeutic interventions.
Immunotoxicology Studies: The panel allows researchers to assess the impact of environmental factors, chemicals, or pharmaceuticals on the immune system of guinea pigs by analyzing changes in cytokine expression profiles.
Autoimmune Disease Studies: By measuring cytokine levels in guinea pigs with autoimmune diseases, researchers can gain insights into the underlying mechanisms of disease development, progression, and potential therapeutic interventions.
Cancer Research: The panel aids in studying the immune response in guinea pig models of cancer, identifying cytokine biomarkers associated with tumor growth, metastasis, and response to treatment.