PLK signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of PLK signaling pathway.

Polo-like Kinases (PLk) are key regulators of the cell cycle and multiple aspects of mitosis. Two agents that inhibit the PLk signaling pathway have shown promising activity in patients with hematologic malignancies and are currently in phase III trials.

Polo-Like Kinase (PLK) Signaling Pathway Detection ServicePLk belongs to a family of serinelfhrconine kinase that are highly conservedin organisms from yeast to human. PLk is a key regulator of cell division in enkaryotic cells PLk intolves in the regulation of different processes of mitosis , including mitotic entry , spindle formation anccytokines. Spatial and temporal coordination of PLk activity is needed in agreement with its abroad functionsduring cell division , and this is achieved through its banding to phosphorylated dcking proteins with distincsubcellular localization,Morcover , PLk is also reported to have functions in both G and mitotic DNA damagcheckpoints. PLk is necessary for mitotic entry following recovery from DNA damage . And mitotic cells with DNA damage agents inhibits PLk activity through dephosphorylation of PLk. Recent findings are uncoveringthe mechanisims of PLk regulation , especially its inyolvement in the DNA damage checkpoint. PLk is overexpressed in lots of humarcancers , and overexpressin of PLk promotes neplastic transformation of human cells. PLk has beeridentified as a promising target for the molecular therapy of neoplasia.

PLk belongs to the highly conserved Serllhr kinase family. They exist in various eukaryotes from yeast to mammals. The first Polo family member discovered is the Pblo protein in fruit bees, and it was subsequently found to have Protein kinase activity, mutation of Polo protein causes abnormal assembly of the metamitotic spindle. In Drosophila melanogaster, Saccharomyces cerevisiae and Schizosaccharomyces cerevisiae contain only one PLk protein, Polo Cdc5 and Plo31, respectively. Pblo-like kinases in mammals include 4 proteins, Plk1, Plk2, Plk3 and SAK (Plk4), of which Plk1 protein performs Polo Most of the functions of Cdc5 and Plo3'. Therefore, the structure and function of Plk1 are currently receiving great attention.

Our detectable targets:

MSK2SLP76p38MAPKMYD88Rac1TLR4
Histone-H3IRF5MEK3mTORSH2TRAF6
ATG16WIPi2LC3IPROLC3ULKFIP200
ISGF3IRS2MSK1p65Tak1TBK1
TRIFVPS34UYRAGNFκBRIG-1p50
VavIRF9AMBRA1p38RIP1TRAF5
ATG3IRS1MEK6PKRISRETRAM

Technology platform:

We provide Luminex technology for Plk signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

As a crucial part of the cell cycle, the precise regulation of mitosis is precisely and strictly regulated,and along with the exploration in the regulation of mitosis, the understanding of life has deepened gradually as well. Plk is involved in different processes of mitosis.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of PLK signaling pathway detection:

Advantages of PLK signaling pathway detection

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing PlK signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Petionczki M ,Lenait P ,Peters J M, et al. Pblo on the rise2 from mitotic entry to cytokinesis with Plk. Dev Cell, 2018, 14 (5): 6462659.
  2. Toyoshime2Morimpto F, Taniguchi E, et al. Plk promotes nuclear translocation of human Cdc25C during prophase. EMBO Rep, 2002, 3(4): 3412348.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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