NGF signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of NGF signaling pathway.

Nerve Growth Factor (NGF) Signaling Pathway Detection Service(Rao S S, et al., 2018)Nerve growth factor (NGF) is a member of the neurotrophic factor family and has a very important role in the survival, differentiation, growth, and repair and regeneration of neurons at different times. NGF has been detected not only in the nervous system, but also in other normal and tumor tissues of human, and the application of NGF in various aspects has been emphasized and confirmed.

Nerve growth factor has a robust synaptic trophic effect that regulates synaptic signal transmission, such as the expression of synaptic vesicle protein, the release of neurotransmitters, the expression of synaptic receptors and the change of synaptic plasticity. The function of NGF is inseparable from its receptor binding. According to the different binding ability of NGF surface glycoprotein and lectin, its receptor can be divided into high-affinity receptor tyrosine kinase A and low-affinity receptor p75. These two receptors interact with nerve growth factor respectively and regulate different signal pathways. The signal pathways mediated by the combination of TrkA and NGF mainly include: (1)MAPK pathway; (2)PLC-γ pathway; (3)PI3K/PKB pathway. The signal transduction pathways mediated by the combination of p75 and NGF mainly include: (1)NF-KB pathway; (2)JNK-p53-Bax apoptosis pathway; (3)ceramide pathway.

NGF and its receptors directly or indirectly participate in the pathogenesis of tumors and the mechanism of cancer pain. However, the role of NGF and its receptors in tumor development and metastasis still needs to be further studied. Therefore, interfering with the expression of this factor and its receptor or blocking its transmission pathway, as well as using it as a target to control the progression of related diseases and other biological behaviors are the focus of current research.

Our detectable targets:

RTKMAPKTRAMp38RIP1p38MAPK
PI3KIRF3SLP76MEKK1PSGL1MEK3
TYK2CREBp50IRF9TRAF5PKR 
STAT1Rac1MEK6GASIRS2mTOR
STAT2MYD88IRS1TRAF3MSK1ISRE
STAT3JNKMHC-IIRIG-1TLR3Fas
TNFRIRAK4TLR9NFκBTak1TRAF6
ISGF3STAT5RelMda-5TRIFSH2
MSK2VavNAP1icam1CD3 
PI3KTBK1TLR4TCRIRF5 

Technology platform:

We provide Luminex technology for NGF signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.

In addition to providing customers with luminex multiplex assay, we can also provide enzyme-linked immunosorbent assay (ELISA), flow cytometry (FACS analysis) technology to meet other customer needs.

Advantages of NGF signaling pathway detection:

Advantages of NGF signaling pathway detection

Application of our service:

Creative Proteomics has developed a signaling pathway target detection platform. We can provide detection services not only for NGF signaling pathways, but also for other signaling pathways. If you want to detect other targets, please contact us in advance and we will customize the service for you according to your requirements. Looking forward to working with you.

References:

  1. Yue XJ, Xu LB, et al. Over-expression of nerve growth fac-tor- β in human cholangiocarcinoma QBC939 cells promote tumor progression. PLoS One, 2017, 8(4): c62024.
  2. Govoni S, Pascale A, et al. NGF and heart: Is there a role in heart discase. Pharmacol Res, 2019, 63(4): 266-277.
  3. Chiaretti A, Falsini B, et al. Neuroprotective role of nerve growth factor in hypoxicischemic injury from brain to skin. Archives ltaliennes de Biologie, 2019, 149(2): 275-282.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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