ROCK signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of ROCK signaling pathway.

Rho-associated kinase (ROCK) is currently the most detailed functional study of Rho downstream target effect molecules. The ROCK signaling pathway induces cytoskeletal reorganization, cell migration and stress fiber formation, and has various physiological functions such as endothelial permeability, tissue contraction and growth. Related, involved in the occurrence of diseases such as diabetic nephropathy, eye diseases, tumors, heart disease, neurological damage diseases, hypertension, radiation damage and leukemia, as a target of drug research and development, people are getting more and more attention.

Rho-associated Kinase (ROCK) Signaling Pathway Detection Service(Wang Y, Zheng XR, et al., 2019)The ROCK signal path includes:

Various extracellular inhibitory signals (such as Nogo-A) combine with p75NTR to form an NgR-p75NTR complex and are transmitted in the cytoplasm, which then activates Rho factor, turning it from inactive Rho-GDP to active Rho-GTP The latter in turn activates ROCK, which leads to the collapse of growth cones by affecting the actin-globulin system, inhibiting nerve cell proliferation and cell regeneration.

ROCK signaling pathway may be involved in the remodeling of actin cytoskeleton and cell morphology changes in breast cancer cells caused by hypoxia.

Our detectable targets:

MSK2SLP76p38MAPKMYD88Rac1TLR4
ATG16WIPi2LC3IPROLC3ULKFIP200
TRIFVPS34UYRAGNFκBRIG-1p50
VavIRF9AMBRA1p38RIP1TRAF5
Histone-H3IRF5MEK3mTORSH2TRAF6
ATG3IRS1MEK6PKRISRETRAM
ISGF3IRS2MSK1p65Tak1TBK1

Technology platform:

We provide Luminex technology for ROCK signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform, developed on the basis of color microspheres, laser technology, applied fluid science and high-speed digital signal processing technology. It core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions were performed on microspheres with different fluorescence codes. The microsphere coding and reporting fluorescence were analyzed qualitatively and quantitatively by laser detection.

The ROCK signal pathway is a ubiquitous pathway in the central nervous system, and it is an important way to mediate inhibitory signals and block the regeneration of central nerve cells.

In addition to Luminex Multiplex Assay, Flow cytometry (FACS analysis), Enzyme-linked immunosorbent assay (ELISA) technology can also be provided to meet other customer needs.

Advantages of ROCK signaling pathway detection:

Advantages of ROCK signaling pathway detection

Application of our service:

Creative Proteomics has developed a signaling pathway target detection platform. We can provide detection services not only for apoptotic signaling pathways, but also for other signaling pathways. If you want to detect other targets, please contact us in advance and we will customize the service for you according to your requirements. Looking forward to working with you.

References:

  1. Wang Y, Zheng XR, et al. ROCK isoform regulation of myosin phosphatase and contractility in vascularsmooth muscle cells. Cire Res, 2019, 104(4): 531-540.
  2. Zohrabian VM, Forzani B, et al. ROCK and MAPK signaling pathways are involved in glioblastoma cell migration and proliferation. Anticancer Res, 2019, 29(1): 119-123.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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