Th17 Cell Differentiation signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of Th17 cell differentiation signaling pathway.

Th17 cells are a subgroup of cells that secrete IL-17 (ie IL-17A) discovered in 2003, and together with Th1, Th2, and Tregs, they constitute four subgroups of CD4+ T cells. Th17 cells are differentiated from Th0 cells under the stimulation of IL-6 and TGF-β. It plays a key role in immune regulation, host defense and autoimmune diseases.

The effector molecules produced by Th17 cells mainly include IL-17A and IL-17F. IL-17A and IL-17F have similar functions and can act on a variety of cell types to induce the production of cytokines, chemokines and metalloproteinases. In addition, Th17 cells also secrete IL-21 and IL-22. IL-21 regulates the differentiation of Th17 in an autocrine form. IL-22 exerts its effects through its receptor.

Th17 Cell Differentiation Signaling Pathway Detection Service

The main function of Th17 cells is to promote the mobilization, recruitment and activation of neutrophils, and to mediate pro-inflammatory reactions. It is closely related to the occurrence of autoimmune diseases, infectious diseases and tumors.

The positive regulators of this pathway include: transforming growth factor β (TGF-β), IL-6, IL-9, IL-1, IL-23, IL-21, IRF4, STAT3, RORα and RORγt. Negative regulators include: IL-2, IL-4, IL-27, STAT1, STAT5, suppressor of cytokine signaling 3 (Socs3), Ets-1 and interferon gamma (IFN-γ).

Th17 cell differentiation mainly includes three stages: induction, expansion and stabilization. At each stage, the differentiation of Th17 cells is finely and complicatedly regulated by a variety of cytokines and signaling molecules.

Our detectable targets:

IL-27IL-2IL-23IL-6TGFBIL-15
STATSSTAT1STATGJUSAHKIKK
Tyk2Tyk1STAT3mTCRp52IRF4
IRF6HIF-1IL-22T-betIL-22IL-21
GATA3FOXP3RXRp38SMADS 

Technology platform

We provide Luminex technology for Th17 cell signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Each kind of microspheres is covalently cross-linked. Capture antibodies against specific antigens.

Th17 cells are differentiated from Tho cells under the stimulation of IL-6 and TGF-β. It plays a key role in immune regulation, host defense and autoimmune diseases.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of Th17 cell signaling pathway detection:

Advantages of Th17 cell signaling pathway detection

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing Th17 cell differentiation signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Mandy J.McGeachy, Daniel J.Cua1, et al. Th17 Cell Differentiation: The Long and Winding Road. Immunity, 2018, 28(4): 445-453.
  2. Dong, C. Genetic controls of Th17 cell differentiation and plasticity. Exp Mol Med, 2019, 43: 1–6.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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