Introduction to exosomes signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of exosomes signaling pathway.

 Exosomes Signaling Pathway Detection Service  Exosomes are small membrane vesicles (30-150nm) that contain complex RNA and proteins. Exosomes were first found in sheep reticulocytes, and a variety of cells can secrete exosomes under normal and pathological conditions. It is mainly derived from multivesicular bodies formed by intracellular lysosomal particles, which are released into the extracellular matrix after fusion of the outer membrane of multivesicles with the cell membrane.Exosomes can regulate the biological activity of recipient cells through the proteins, nucleic acids, lipids, etc.

The communication between cells mediated by exosomes is mainly through the following three methods:

The function of exosomes depends on the cell type from which they are derived, and they can participate in all aspects of the body's immune response, antigen presentation, cell migration, cell differentiation, and tumor invasion. Studies have shown that tumor-derived exosomes participate in the exchange of genetic information between tumor cells and basal cells, leading to the formation of a large number of new blood vessels and promoting tumor growth and invasion.

Our detectable targets:

RTKPI3KTYK2STAT1STAT2STAT3
STAT5IRAK4JNKMYD88Rac1TLR4
CREBIRF3MAPKNAP1RelTLR9
TCRicam1Mda-5NFκBRIG-1TRAF3
GASIRF9MEKK1p38RIP1TRAF5
CD3IRF5MEK3p38MAPKSH2TRAF6
MHC-IIIRS1MEK6p50SLP76TRAM
PSGL1IRS2MSK1TLR3Tak1TRIF
TNFRISGF3MSK2PI3KTBK1Vav
FasISREmTORPKR

Technology platform:

We provide Luminex technology for exosomes signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

The exosomal pathway is widely used in the body's immune response, antigen presentation, cell migration, cell differentiation, tumor invasion, and promotion of tumor growth and invasion.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of exosomes signaling pathway detection:

Advantages of exosomes signaling pathway detection

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing exosomes signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Suresh Mathivanan, Hong Ji, et al. Exosomes: Extracellular organelles important in intercellular communication. Journal of proteomics, 2018, 7(3): 1907-1920.
  2. 2. Richard J Simpson, Justin WE Lim,et al. Exosomes: proteomic insightsand diagnostic potential. Expert Rev. Proteomics, 2019, 6(3): 267-283.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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