In the realm of microbial analysis, conventional culture-based methods have historically been the go-to approach. Such methods are centered on cultivating microorganisms on selective media, which not only demands a lot of time but also proves inadequate in terms of sensitivity and specificity. Besides, the customary techniques are limited in their ability to identify microbial species and are not effective in detecting certain fastidious or unculturable organisms.
To surmount these challenges, alternative techniques, such as molecular-based methods, have emerged. These approaches are predicated on detecting specific microbial nucleic acid sequences, enabling swift and accurate identification of microbial species. However, they are often constrained by the number of targets that can be analyzed simultaneously, necessitating the use of multiple assays to identify a broad range of microorganisms.
Enter the Luminex Multiplex Technology, a ground-breaking platform that enables the analysis of multiple targets in a single assay, affording high throughput and efficient examination of various microbial species. Based on microsphere beads, each encoded with a distinct fluorescent signature, the Luminex technology permits the detection of specific targets amidst a mixture of diverse targets.
One of the primary merits of the Luminex technology is its capacity to simultaneously detect multiple targets, substantially curbing analysis time and cost compared to traditional methods. Moreover, the technology boasts high sensitivity and specificity, facilitating the detection of low-abundance targets with minimal interference from non-specific binding.
Further, the Luminex technology is highly versatile, lending itself to the detection of a wide array of targets, including proteins, nucleic acids, and small molecules, making it highly adaptable to different applications. Additionally, the technology is highly customizable, allowing for the development of panels tailored to meet specific user needs.
Microbial Luminex Multiplex Technology Platform in Creative Proteomics
Creative Proteomics can provide high quality and accurate results for microbial analysis using the Luminex Multiplex technology platform, making it an invaluable tool for clinical diagnostics, research and environmental monitoring.
Our instruments and equipment, such as the Luminex 200/100/50/FlexMAP 3D and xMAP INTELLIFLEX, offer modular design, enhanced sensitivity and high throughput analysis. The platform also includes Luminex xPONENT software, which controls the instrument and allows for data acquisition and analysis, and a magnetic separation rack, which separates the microspheres from the sample matrix and wash buffer. Luminex calibration kits are used for instrument calibration, and quality control samples are run with the test samples to ensure accuracy and reproducibility. The parameters used can be optimized for each sample to maximize sensitivity, specificity and dynamic range.
FLEXMAP 3D ® Systems
xMAP INTELLIFLEX® Systems
What Specific Panels Are Included in the Luminex Microbial Multiplex Panel?
The Luminex Microbial Multiplex Panel offered by Creative Proteomics includes several panels that are designed to detect and identify specific groups of microorganisms. These panels include:
- Bacterial Identification Panel: This panel is designed to identify and differentiate several important bacterial species, including Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, among others.
- Fungal Identification Panel: This panel is designed to identify and differentiate several important fungal species, including Candida albicans, Aspergillus fumigatus, and Cryptococcus neoformans, among others.
- Parasite Identification Panel: This panel is designed to identify and differentiate several important parasitic species, including Plasmodium falciparum, Toxoplasma gondii, and Giardia lamblia, among others.
- Viral Identification Panel: This panel is designed to identify and differentiate several important viral species, including human papillomavirus (HPV), herpes simplex virus (HSV), and influenza virus, among others.
Each panel includes a set of specific targets that can be analyzed simultaneously, allowing for rapid and accurate identification of a broad range of microorganisms.
In addition to the pre-designed panels, Creative Proteomics also offers the option for custom panel design, allowing for the detection of specific targets relevant to the user's research or application.