ROS1 Signaling Pathway Luminex Multiplex Assay

Introduction to ROS1 signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of ROS1 signaling pathway.

The ROS1 gene is located in the q21 region of chromosome 6. The full-length cDNA contains 44 exons and encodes 2347 amino acids. The insulin receptor is a member of the RTKs family ROIS1 and ALK. 77% of the tyrosine kinase domains are identical. Therefore, the ALK small molecule inhibitor crizotinib has obvious curative effect in the treatment of NSCLC with fusion mutation of ROS1. In terms of pathogenic mechanism, despite the lack of suitable ligands or small molecule activators for ROS1 activation, the EGFR-ROS1 chimeric protein expression technology constructed using point mutations and EGFR extracellular receptor parts has been proved that ROS1 receptor tyrosine Kinases are involved in the activation of multiple downstream signal transduction pathways, including RAS-RAF-MEK-ERK, PI3K-AKT-mTOR and JAK-STAT3 pathways, which are related to tumor cell proliferation, survival and metastasis.

ROS1 is a proto-oncogene and is highly expressed in a variety of tumor cell lines. ROS1 is one of the family members of the tyrosine kinase insulin receptor. The encoded protein is a type I intramembrane protein with tyrosine kinase activity, which can be used as a receptor for growth factors or differentiation factors.

ROS1 can activate signal pathways related to cell differentiation, proliferation, growth and survival, including PI3K-AKT-mTOR pathway. ROS1 can activate downstream signaling pathways by regulating the phosphorylation of downstream pathway proteins, such as PTPN11 (activator of PI3K-mTOR pathway), AKT1, MAPK1, and STAT3 transcription factors.

The common pathogenic mutation of ROS1 gene is gene rearrangement. The resulting ROS1 fusion protein will become a continuously activated tyrosine kinase, which will activate the signals of its downstream pathways, which will cause cell overgrowth and proliferation.

Creative Proteomics provides key protein analysis services for the ROS1 Signaling Pathway, which plays an important role in a variety of biological processes, such as cell proliferation, growth, differentiation, and survival. ROS1 Signaling Pathway protein analysis can provide insight into the specific regulatory mechanisms and interactions of the ROS1 signaling pathway in these processes, providing an important reference for the diagnosis, treatment and drug development of related diseases. In addition, we can provide valuable scientific data for researchers in related disciplines to better understand the role and mechanism of the ROS1 signaling pathway.

Our detectable targets:

Technology platform:

We provide Luminex technology for ROS1 signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

ROS1 gene is a kind of proto-oncogene, and chromosome rearrangement is the primary mechanism of ROS1 activation. ROS1 can activate signaling pathways related to cell differentiation, proliferation, growth and survival. ROS1 signaling pathway is widely used in medical research.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of ROS1 signaling pathway detection:

• High sensitivity: The surface area of the microspheres is large, and 100,000 probes can be fixed on each microsphere, which ensures the maximum binding with the target molecules in the sample and improves the detection sensitivity. The lowest detection concentration can reach 0.1pg/mL.
• Strong specificity: Can read the fluorescent signal on a single microsphere, and automatically distinguish between bound and unbound microspheres.
• High accuracy: Without washing, the fluorescence intensity of the reporter molecule on the microsphere is proportional to the bound molecule to be detected. Because the detection range of the Luminex detection platform is large, it does not need to be diluted like ELISA detection, which further reduces errors.

Application of our service:

• To study the regulation mechanism of ROS1 signal pathway in disease
• To study the effect of each virus on ROS1 signaling pathway
• To study the effects of drugs or therapies on ROS1 signaling pathways

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing ROS1 signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

Reference:

1. Drilon, A., Jenkins, C., et al. ROS1-dependent cancers - biology, diagnostics and therapeutics. Nat Rev Clin Oncol, 2021, 18: 35-55.