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Introduction

Interleukin 13 (IL-13) is mainly secreted by activated CD4+ Th2 cells, and non-T cells such as mast cells, eosinophils and basophils can also produce IL-13. In the early allergic reaction, IL-13 mainly comes from natural killer T cells (NK cells). Its main biological function is to promote B cell proliferation and differentiation, induce B cell synthesis of IgE and expression of CD23, MHC class II molecules, downregulate cytotoxicity and inflammation of monocytes and macrophages, and play an important role in regulating immune and inflammatory responses. IL-13 has complex biological functions and participates in the occurrence and pathogenesis of various diseases. With the deepening of research, it has been found that IL-13 is closely related to dermatological diseases such as psoriasis, atopic dermatitis, lupus erythematosus, urticaria and allergic purpura. If it can be further studied its mechanism of action in related skin diseases, it will play an important role in promoting the development of dermatology.

Mechanism and Function

IL-13 is produced by TH2 cells and has a molecular weight of approximately 10KD. Both human and mouse-derived IL-13 are single-copy genes and contain 4 exons and 3 introns. The hIL-13 and mIL-13 genes show a high degree of identity at the 5 'end and contain a large number of consistent sequences of transcription factors, which play an important role in regulating the expression of the genome. The IL-13 gene is located on chromosome 5 and is tightly linked to the IL-4 gene. The amino acid sequence of IL-13 molecule has 20% to 25% homology with IL-4, and has many similarities with IL-4 in function. IL-13 can induce the differentiation of monocytes and enhance the expression of MHC class II molecules; inhibit the secretion of monocyte factors induced by lps to control the inflammatory response; induce the proliferation of B cells and synthesize IgE antibodies, enhance the MHC class II molecules and CD23 on the surface of B cells And CD72 expression; cooperate with IL-2 to stimulate NK cells to produce IFN, thereby promoting monocyte-macrophage activation and TH1-type cellular immune response. Recently, it has been found that IL-13 also has multiple functions such as inhibiting HIV-1 replication in macrophages, inducing IL-1RA gene expression and protein synthesis in neutrophils.

The various functions of IL-13 are completed by a complex receptor system, including IL-4 receptor α (IL-4Rα, CD124) and two other cell surface proteins of the same family: IL-13 receptor α1 (IL-13Rα1, CD231α1) and IL-13 receptor α2 (IL-13Rα2, CD231α2). In IL-13R, IL-13Rα1 and IL-4Rα exist as homodimers. IL-13Rα2 is considered to be an inducing receptor due to its shorter intracellular protein form. IL-13Rα2 exists in three forms: membrane protein, intracellular protein and extracellular soluble protein. IL-13 uses JAK-STAT to establish signaling pathways. After IL-13 binds to IL-13R, it leads to the activation of JAK1 and Tyk2. JAK can cause the phosphorylation of IL-4Rα intracellular tyrosine, which in turn phosphorylates and activates STAT6. The activated STAT6 dimer is transferred into the nucleus, which in turn binds DNA to regulate the transcription of downstream genes.

IL-13 Detection Service Fig 1. Mechanism of Signaling

Creative Proteomics can provide cytokine detection platform for scientific research. According to different purposes, our dedicated analysts will customize exclusive solutions for you. We aim to provide customers with high-quality and convenient services to help you accelerate the progress of your project.

Our cytokine detection service includes but is not limited to:

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We mainly provide the Luminex cytokine detection platform. Luminex uses fluorescently encoded microspheres with specific antibodies to different target molecules. The different microspheres can be combined freely to a certain extent so that up to 100 analytes can be tested multiple times simultaneously in a single experiment.

The Luminex cytokine assay platform has the following advantages:

For your different needs, we can also provide the following detection methods:

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IL-13 Detection Service

For more information about the IL-13 detection service or need other detection requirements, please contact us.

References:

  1. Wills-Karp M. Interleukin-13 in asthma pathogenesis. Curr Allergy Asthma Rep, 2004, 4: 123-131.
  2. Andrews AL, et al. Kinetic analysis of the interleukin-13 receptor complex. J Biol Chem, 2002, 277: 46073-46078.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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