Luminex xMAP Technology Assay

Luminex Assay is a multiplex technology that simultaneously measures multiple analytes from a single sample. It uses color-coded beads with unique spectral codes and a dual-laser system to detect and quantify biomarkers efficiently, offering high sensitivity and low sample volume requirements.

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Luminex Cytokine Panel

What is Luminex xMAP Technology?

Luminex xMAP technology is a bead-based multiplex assay platform that leverages the power of color-coded microspheres to perform high-throughput, simultaneous detection of up to 100 different analytes. This system combines advanced flow cytometry with proprietary bead technology to offer unparalleled flexibility and sensitivity in biomarker analysis.

Core Components of xMAP Technology

Microsphere Beads: At the heart of xMAP Technology are color-coded microsphere beads. Each bead is coated with specific capture molecules that bind to target analytes. These beads come in a range of colors, allowing for the simultaneous analysis of multiple analytes. The color coding is based on a unique ratio of two different dyes incorporated into each bead.

Laser-Based Detection: After the beads are coated with capture molecules and mixed with the sample containing the target analytes, a laser-based detection system identifies and quantifies the analytes. The system uses two lasers:

  • Identification Laser: This laser reads the color code of the beads, determining which type of bead is present in each event.
  • Reporter Laser: This laser measures the intensity of the fluorescent signal emitted by a reporter molecule attached to the bead, which correlates with the amount of analyte captured.

Data Analysis: The signals are processed by specialized software that interprets the bead color and fluorescence data to provide quantitative results for each analyte. This data can then be used for various applications, from profiling biomarker expression to analyzing genetic variations.

Benefits of Luminex xMAP Technology

  • High Multiplexing Capability

    Measures up to 100 different biomarkers in a single sample, reducing sample volume and speeding up analysis compared to single-plex assays.

  • Reduced Sample Volume

    Requires as little as 12.5 μL per reaction, which is ideal for studies with limited samples.

  • Cost-Effective

    Lowers costs by combining multiple assays into one test, minimizing reagent consumption, labor, and sample handling.

  • High Sensitivity and Specificity

    Utilizes a dual-laser detection system for precise measurement of analytes, even at low concentrations.

  • Versatility

    Applicable to a range of research fields, including oncology, immunology, cardiology, and neurology, supporting various applications like cytokine profiling and gene expression analysis.

Luminex Assay in Creative Proteomics

Customized Your Luminex Multiplex Assay

Maximize efficiency and minimize costs with Creative Proteomics' Luminex multiplex assay, capable of simultaneously analyzing up to 50 biomarkers. Our flexible and user-friendly design tool allows you to easily personalize your multiplex microbead immunoassay panel.

Luminex Multiplex Assay Customization Tool

Preconfigured Panels by Research Area

Species
Human
Mouse
Rat
NHP
Porcine
Canine
Feline
Equine
Analysis Targets
Cytokine/Chemokine
Inflammation
Immunoglobulin Isotyping
Growth Factor
Th17
Th1/Th2
Treg
TGF-β
T Cell
Soluble Cytokine Receptor
Angiogenesis
Cardiovascular
Cancer Biomarker
Obesity
Metabolic
Metabolic Hormone
Coagulation
IGFBP
Liver Protein
Pituitary
Kidney Injury (Urine)
Kidney Injury (Serum/Plasma)
Neurodegenerative Disease
Neurological Disorders
Neuropeptide
MMP
Diabetes
Bone Metabolism
Sepsis
Complement
Myokine
Amyloid beta and Tau
Cardiovascular Disease
MMPs
Kidney Injury
Liver Injury
Adiponectin
Stress Hormone
Thyroid
Adipokine
Adipocyte
Proinflammatory
T-Cell
TH1/TH2
TH17
TGFβ
Cytokine
Metabolism
Kidney Injury
HumanCytokine/Chemokine8-plex PanelIL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, IFN-γ, TNF-α
HumanCytokine/Chemokine10-plex PanelIL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IFN-γ, TNF-α
HumanCytokine/Chemokine14-plex PanelGM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ
HumanCytokine/Chemokine23-plex Panel6Ckine, BCA-1, CTACK, ENA-78, Eotaxin-2, Eotaxin-3, I-309, IL-16, IL-20, IL-21, IL-23, IL-28A, IL-33, LIF, MCP-2, MCP-4, MIP-1δ, SCF, SDF-1, TARC, TPO, TRAIL, TSLP
HumanCytokine/Chemokine48-plex Panel AsCD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A
HumanCytokine/Chemokine48-plex Panel B6Ckine, APRIL, BAFF, BCA-1, CCL28, sCD137, CTACK, CXCL16, ENA-78, Eotaxin-2, Eotaxin-3, sFAS, sFASL, GCP-2, Granzyme A, Granzyme B, HMGB1, I-309, IFNβ, IFNꙍ, IL-11, IL-16, IL-20, IL-21, IL-23, IL-24, IL-28A, IL-29, IL-31, IL-33, IL-34, IL-35, I-TAC, LIF, Lymphotactin, MCP-2, MCP-4, MIP-1Ᵹ, MIP-3α, MIP-3β, MPIF-1, Perforin, SCF, SDF-1, TARC, TPO, TRAIL, TSLP
HumanCytokine/Chemokine71-plex Panel6CKine, BCA-1, CTACK, EGF, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, FGF-2, Flt3L, Fractalkine, G-CSF, GM-CSF, GROα, I-309, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-20, IL-21, IL-22, IL-23, IL-27, IL-28, IL-33, IP-10, LIF, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-AA, PDGF-AB/BB, RANTES, sCD40L, SCF, SDF-1α+β, TARC, TGFα, TNFα, TNFβ, TPO, TRAIL, TSLP, VEGF-A
HumanCytokine/Chemokine96-plex Panel6Ckine, APRIL, BAFF, BCA-1, CCL28, sCD137, sCD40L, CTACK, CXCL16, EGF, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, sFas, sFasL, FGF-2, FLT-3L, Fractalkine, GCP-2, G-CSF, GM-CSF, Granzyme A, Granzyme B, GROα, HMGB1, I-309, IFN-α2, IFNβ, IFNꙍ, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-20, IL-21, IL-22, IL-23, IL-24, IL-27, IL-28A, IL-29, IL-31, IL-33, IL-34, IL-35, IP-10, I-TAC, LIF, Lymphotactin, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG/CXCL9, MIP-1α, MIP-1β, MIP-1Ᵹ, MIP-3α, MIP-3β, MPIF-1, PDGF-AA, PDGF-AB/BB, Perforin, RANTES, SCF, SDF-1, TARC, TGFα, TNFα, TNFβ, TPO, TRAIL, TSLP, VEGF-A.
HumanInflammation20-plex PanelIL-12, CCL2, CCL3, CCL4, CD54, CXCL10, GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-10, IL-13, IL-17A, IL-4, IL-6, IL-8, sCD62E, sCD62P, TNFα
HumanInflammation24-plex PanelAPRIL/TNFSF13, BAFF/TNFSF13B, Chitinase-3-like1, gp130/sIL-6Rβ, IFN-β, IL-11, IL-19, IL-20, IL-26, IL-27(p28), JIL-28A/IFN-λ2, IL-29/IFN-λ1, IL-32, IL-34, IL-35, LIGHT/TNFSF14, Osteocalcin, Pentraxin-3. sCD163, sCD30/TNFRSF8, sTNF-R1, sTNF-R2, TSLP, TWEAK/TNFSF12
HumanInflammation25-plex PanelCCL2/MCP-1, CCL3/MIP-1α, CCL4MIP-1β, CCL11/Eotaxin, CCL24/Eotaxin-2, CXCL8/IL-8, CXCL9/MIG, CXCL13/BLC, G-CSF, GM-CSF, ICAM-1, IFN-γ, IFN-γR1, IL-1α, IL-1Ra, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-15, M-CSF, TNF-α, TNFRI
HumanInflammation37-plex PanelAPRIL/TNFSF13, BAFF/TNFSF13B, Chitinase-3-like1, gp130/sIL-6Rβ, IFN-α2, IFN-β, IFN-γ, IL-2, IL-8, IL-10, L-11, IL-12(p40), IL-12(p70), IL-19, IL -20, L-22, IL-26, L-27(p28), IL-28A/IFN-λ2, IL-29/IFN-λ1, IL-32, IL-34, IL-35, LIGHT/TNFSF14, MMP-1, MMP-2, MMP-3, Osteocalcin, Osteopontin, Pentraxin-3, sCD163, sCD30/TNFRSF8, sIL-6Rα, sTNF-R1, sTNF-R2, TSLP, TWEAK/TNFSF12
HumanImmunoglobulin Isotyping6-plex panelIgA, IgG1, IgG2, IgG3, IgG4, IgM
HumanGrowth Factor11-Plex PanelBDNF, BNGF, EGF, FGF-2(FGFbasic), HGF, LIF, PDGF-BB, PIGF, SCF, VEGF-A, VEGF-D
HumanTh1715-Plex PanelIL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IFN-γ, sCD40L, TNF-α.
HumanTh1725-Plex PanelIL-4, IL-5, IL-6, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17A/CTLA8, IL-17E/IL-25, IL-17F, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-27, IL-28A/IFNλ2, IL-31, IL-33, IFN-, GM-CSF, MIP-3α/CCL20, TNFα, TNFβ
HumanTh1/Th26-Plex PanelIFNγ, IL-4, IL-5, IL-6, IL-12p70, TNF-alpha
HumanTh1/Th29-Plex PanelGM-CSF, IFN-γ, IL-2, IL-4, IL-6, IL-8, IL-10, IL-13, TNF-α
HumanTh1/Th211-Plex PanelGM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12p70, IL-13, IL-18, TNF-α
HumanTh1/Th218-Plex PanelGM-CSF, IFN-α, IFN-γ, IL-1α, IL-1β, I-1RA, IL-2, IL-4, IL-5, L-6, IL-7, -12p70, IL-13, IL-15, IL-18, IL-31, TNF-α, TNF-β
HumanTh1/Th220-Plex PanelEotaxin, GM-CSF, GROα/CXCL1, IFN-γ, IL-1β, IL-2, IL-4, IL-5, L-6, IL-8/CXCL8, IL-12p70, IL-13, IL-18, IP-10/CXCL10, MCP-1/CCL2, MIP-1α/CCL3, MIP-1 beta/CCL4, Rantes, SDF1α/CXCL12, TNF-α
HumanTreg7-Plex PanelIL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27
HumanTreg12-Plex PanelIL-2, IL-10, IL-12(p40), IL-12(p70), IL-19, IL-20, IL-22, IL-26, L-27(p28), II-28A/IFN-λ2, IL-29/IFN-λ1, IL-35
HumanTreg14-Plex PanelIFN-α, IL-1α, IL-1RA, IL-7, IL-9, IL-10, IL-15, IL-17A, IL-21, IL-22, IL-23, L-27, IL-31, TNF-β
HumanTreg16-Plex PanelEotaxin, GROα/CXCL1, IL-8/CXCL8, IL-9, IL-10, IL-17A, IL-21, IL-22, IL-23, IL-27, IP-10/CXCL10, MCP-1/CCL2, MIP-1α/CCL3, MIP-1β/CCL4, Rantes, SDF 1α/CXCL12
HumanTreg18-Plex PanelGM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, TNFα
HumanTGF-β3-Plex PanelTGF-β1, TGF-β2, TGF-β3
HumanT Cell13-Plex PanelIL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8/CXCL8, L-10, IL-12(p70), IL-13, IFNγ, GM-CSF, TNFα
HumanT Cell17-Plex PanelIL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IFNγ, GM-CSF, Granzyme A, Granzyme B, MIP-1α/CCL3, MIP-1β/CCL4, Perforin, sCD137/4-1BB, sFas, sFasL/ TNFRSF6, and TNFα
HumanT Cell21-Plex PanelIL-1β, IL-2, IL-21, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8/CXCL8, IFNγ, IL-10, L-12(p70), IL-13, IL-17A/CTLA8, Fractalkine/CX3CL1, GM-CSF, I-TAC/CXCL 11, MIP-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20, TNFα
HumanSoluble Cytokine Receptor14-Plex PanelsCD30, EGFR, sgp130, sIL-1R I, sIL-1R II, sIL-2Rα, sIL-4R, sIL-6R, sRAGE, sTNFR I, sTNFR II, sVEGFR1/sF1t-1, sVEGFR2/sKDR/sFlk-1, sVEGFR3/sFlt-4
HumanAngiogenesis17-Plex PanelAngiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1/FGF- acidic, FGF-2/FGF- basic, Follistatin, G-CSF, HB-EGF, HGF, IL-8/CXCL8, Leptin, PLGF VEGF-A, VEGF-C, VEGF-D
HumanAngiogenesis20-Plex PanelAngiostatin/Kringle, Osteopontin (OPN), PDGF-AB/BB, sAXL, SCFR, sEGFR/sHER1/sErbB1, sE-Selectin, sHER2/sEGFR2/sErbB2, sHER3/sEGFR3, sHGFR/sc- Met, sIL-6Ra, sNeuropilin-1 (sNRP-1), sPECAM-1, sTIE-2, suPAR, sVEGFR1/sFlt1, sVEGFR2 /sFIk-1, sVEGFR3/sF1-4, TenascinC (TN-C), Thronmbospondin -2(TSP-2)
HumanCardiovascular6-Plex PanelApoAI, ApoA II, ApoB, ApoC I, ApoC III, ApoE
HumanCardiovascular8-Plex PaneldPAPP-A, Follistatin (FST), Pentraxin-3 (PTX3), sCD31/sPECAM-1, sE-Selectin, Thrombomodulin, Tissue Factor (TF), Troponin T (TnT)
HumanCardiovascular10-Plex PanelADAMTS13, D-dimer, GDF-15, Myeloperoxidase (MPO), Myoglobin, NGALLipocalin-2, SerumAmyloidA.sICAM-1, sP-Selectin, sVCAM-1
HumanCardiovascular11-Plex PanelAdipsin, CRP, FetuinA, Fibrinogen, Haptoglobin, Platelet Factor4 (PF4), Senum AmyloidP, sL-Selectin, vonWillebrand Factor (vWF), α1-AcidGlycoprotein (AGP), α-2-MacroglobulinF
HumanCardiovascular12-Plex PanelCK-MB, CXCL16, Endocan (ESM-1), FABP3, FABP4, LIGHT, LIX/CXCL6/GCP-2, NP, NTproBNP, Oncostatin (OSM), Placental Growth Factor (PLGF), TroponinI (TnI)
HumanCancer Biomarker5-Plex PanelAntithrombin III, Complementary factor H (CFH), Extracellular matrix protein 1 (ECM1), Vitamin D binding protein, Vitronectin
HumanCancer Biomarker8-Plex PanelCathepsin D, Ferritin, Fibroblast activation protein (FAP), Galectin3, IGFBP3, Melanoma inhibitory activity (MIA), Myeloperoxidase (MPO), Sex hormone-binding globulin
HumanCancer Biomarker9-Plex PanelDKK1, GDF15, Neuron-specificenolase (NSE), Osteonectin (SPARC), Osteoprotegerin (OPG), Periostin, TRAP, TWEAK, YKL40
HumanCancer Biomarker10-Plex PanelALDH1A1, Carbonic Anhydrase 9, CD44, EpCAM, Hepsin, Kallikrein-6, Mesothelin, Midkine, NCAML/L1CAM/CD171, Transglutaminase 2 (TGM2)
HumanCancer Biomarker24-Plex PanelCA125, CA15-3, CA19-9, CEA, CYFRA21-1, FGF-2/FGF-basic, HCGβ, HE4, HGF, IL-6, IL- 8/CXCL8, Leptin, MIF, Osteopontin (OPN), Prolactin, PSA (total), SCF, sFas, sFasL/TNFRSF6, TGFα, TNFα, TRAIL/TNFSF10, VEGF-A, α-Fetoprotein (AFP)
HumanObesity5-Plex PanelAdiponectin, Adipsin, NGAL/Lipocalin-2, PAI-1 (total), Resistin
HumanObesity9-Plex PanelHGF, IL-1β, IL-6, IL-8/CXCL8, Insulin, Leptin, MCP-1/CCL2, NGF, TNFα
HumanObesity11-Plex PanelAdiponectin, HGF, IL-1β, IL-6, IL-8/CXCL8, Leptin, MCP-1/CCL2, NGF, PAI-1(total), Resistin, TNFα
HumanMetabolic5-Plex PanelCortiso1, C-Peptide, Peptide YY, PP, Proinsulin
HumanMetabolic5-Plex PanelANGPTL3, Chemerin, DPP4, ProteinS, SEPP
HumanMetabolic7-Plex PanelAAT, AGP-1, Hemopexin, RBP-4, Transferrin, Transthyretin, VDBP
HumanMetabolic8-Plex PanelFGF-21, FGF-23, Galectin-3, Omentin-1, Pentraxin-3, PON-1, sLeptinR, Vaspin
HumanMetabolic Hormone12-P1ex PanelAmylin (total), C-Peptide, Ghrelin(active)%, GIP (total), GLP-1 (total), L-6, Insulin, Leptin, MCP-1/CCL2, Pancreatic Polypeptide, PYY (total), TNFα
HumanCoagulation6-Plex PanelFactor XI, Factor XII, Factor XIII, Antithrombin, Prothrombin, CRP
HumanIGFBP7-Plex PanelIGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, IGFBP7
HumanLiver Protein9-Plex PanelANGPTL3, ANGPTL4, ANGPTL6, FABP1, FGF-19, FGF-21, FGF-23, HGF, alpha-Fetoprotein
HumanPituitary7-Plex PanelACTH, Agouti-Related Protein (AgRP), CNTF, FSH, GH, LH, TSH
HumanKidney Injury (Urine)3-Plex PanelRBP4, Uromodulin, β-2-Microglobulin
HumanKidney Injury (Urine)6-Plex PanelCalbindin, Clusterin, GST-pi, IL-18, KIM-1, MCP-1
HumanKidney Injury (Urine)6-Plex PanelAlbumin, B2M, CystatinC, NGAL, Osteopontin, TFF3
HumanKidney Injury (Urine)7-Plex PanelAlbumin, Clusterin, CystatinC, EGF, NGAL, Osteopontin (OPN), α-1-Microglobulin
HumanKidney Injury (Urine)11-Plex PanelCalbindin, CollagenIV, FABP1, GSTα, GSTπ, IP-10/CXCL10, KIM-1, Osteoactivin, Renin, TFF-3, TIMP-1
HumanKidney Injury (Serum/Plasma)4-Plex PanelClusterin, CystatinC, RBP4, β-2-Microglobulin
HumanKidney Injury (Serum/Plasma)7-Plex PanelCollagenlV, GSTπ, NGA, Osteoactivin, TIMP-1, Uromodulin, alpha-1-Microglobulin
HumanKidney Injury (Serum/Plasma)8-Plex PanelEGF, FABP1, GSTα, IP-10/CXCL10, KIM-1, Osteopontin (OPN), PTH, Renin
HumanNeurodegenerative Disease5-Plex PanelAβ1-40, Aβ1-42, GDNF, S100B, sRAGE
HumanNeurodegenerative Disease6-Plex Panelα-2-Antitrypsin, Complement C4, CRP, MIP-4/PARC, Serum Amyloid P (SAP), PEDF
HumanNeurodegenerative Disease7-Plex Panelα-2-Macroglobulin, ApoA I, ApoC III, ApoE, Complement C3, Complement factor H (CFH), Prealbumin/Transthyretin (TTR)
HumanNeurodegenerative Disease10-Plex PanelBDNF, CathepsinD, Myeloperoxidase (MPO), PAI-1 (total), PDGF-AA, PDGF-AB/BB, RANTES/CCL5, sICAM-1, sNCAM, sVCAM-1
HumanNeurological Disorders4-Plex PanelCeruloplasmin (CP), Haptoglobin (HP), Serum Amyloid P, α-1-AcidGlycoprotein (AGP)
HumanNeurological Disorders7-Plex PanelAngiotensinogen (AGT), Contactin-1, FetuinA, Kallikrein-6, Osteopontin (OPN), Soluble Superoxide Dismutase1 (sSOD1), Soluble Superoxide Dismutase2 (sSOD2)
HumanNeuropeptide6-Plex PanelNeurotensin, OrexinA, Oxytocin, SubstanceP, α-MSH, β-Endorphin
HumanMMP3-Plex PanelMMP-3, MMP-12, MMP-13
HumanMMP5-Plex PanelMMP-1, MMP-2, MMP-7, MMP-9, MMP-10
HumanMMP9-Plex PanelMMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, MMP-13
HumanDiabetes4-Plex PanelC-Peptode, GLP-1(active), Insulin, Leptin
HumanDiabetes9-Plex PanelC-peptide, ghrelin, GIP, GLP-1, insulin, leptin, PAI-1 (total), resistin, visfatin
HumanBone MetabolismSingle-Plex PanelRANKL
HumanBone Metabolism13-Plex PanelACTH, DKK1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteopontin (OPN), Osteoprotegerin (OPG), PTH, Sclerostin (SOST), TNFα
HumanSepsis2-Plex PanelElastase-2, Proteinase-3 (PRTN-3)
HumanSepsis3-Plex PanelFGF-13, MMP-8, Olfactomedin-4 (OLFM-4)
HumanSepsis5-Plex PanelLactotransferrin (LTF), Neutrophil Elastase-2 (ELA2), NGA, Resistin, Thrombospondin-1
HumanSepsis6-Plex PanelMIF, PAI-1 (tota1), sFas, sFasL/TNFRSF6, sICAM-1, sVCAM-1
HumanSepsis7-Plex PanelGranzyme B, HSP70, L-1α, IL-8/CXCL8, MIP-1α/CCL3, MIP-1β/CCL4, MMP-8
HumanComplement7-Plex PanelC1q, C3, C3b, C4, FactorB, FactorH, Properdin
HumanComplement8-Plex PanelAdipson/FactorD, C2, C4b, C5, C5a, C9, Factor I, Mannose-bindinglectin (MBL)
HumanMyokine15-Plex PanelApelin, BDNF, Fractalkine/CX3CL1, Erythropoietin (EPO), LIF, IL-15, SPARC/Osteonectin, Myostatin, FABP3, Irisin, FSTL-1, IL-6, Osteocrin/ Musclin, Oncostatin-M, FGF-21
HumanAmyloid beta and Tau4-Plex PanelAβ1-40, Aβ1-42, Tau (Thr181), Tau (total)
MouseCytokine/Chemokine10-Plex PanelGM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, MCP-1, TNFα
MouseCytokine/Chemokine18-Plex PanelGM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12p70, IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNFα
MouseCytokine/Chemokine31-Plex PanelEotaxin, G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα, VEGF-A
MouseCytokine/Chemokine44-Plex PanelEotaxin, Erythropoietin, 6Ckine, Fractalkine, G-CSF, GM-CSF, IFNB1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, KC, LIF, LIX, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-2, MIP-3α, MIP-3B, RANTES, TARC, TIMP-1, TNFα, VEGF-A
MouseCardiovascular Disease3-Plex PanelNGAL/Lipocalin-2, Pentraxin-3 (PTX3), Serum Amyloid A-3 (SAA-3)
MouseCardiovascular Disease6-Plex PanelAdipsin, α1-AcidGlycoprotein (AGP), CRP, Haptoglobin, Serum Amyloid P, α-2-Macroglobulin
MouseCardiovascular Disease7-Plex PanelMMP-9, PAI-1(total), sCD31/sPECAM-1, sE-Selectin, sICAM, sP-Selectin, Tomomodulin
MouseCardiovascular Disease9-Plex PanelCXCL16, Endocan-1, Follistatin (FST), LIGHT, Oncostatin-M, Placental Growth Factor (PLGF-2), sCD40L, Troponin I (Tn I), Troponin T (TnT).
MouseCancer Biomarker27-Plex PanelsALK-1, Amphiregulin, Angiopoietin-2Δ, BetacellulinΔ, sCD31/sPECAM-1, EGF, Endoglin, Endothelin-1, sFasL/TNFRSF6, FGF-2/FGF-basic, Folliststin(FST), G-CSF, HGF, IL-1β, IL-6, IL-17A/CTLA8, KC/CXCL1, Leptin, MCP-1/CCL2, MIP-1α/CCL3, Placental Growth Factor(PLGF-2), Prolactin SDF-1/CXCL12, TNFα, VEGF-A, VEGF-C, VEGF-D
MouseTh176-Plex PanelIL-1β, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF-α.
MouseTh178-Plex PanelCD40L, IL-17F, IL-21, IL-22, IL-23p19, IL-31, IL-33, MIP-3α
MouseTh1725-Plex PanelL-4, IL-5, IL-6, IL-10, IL-12 (p70), L-13, L-15, IL-17A/CTLA8, L-17E/IL-25, IL-17F, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-27, IL-28B/IFNλ3, IL-31, IL-33, IFNγ, GM-CSF, MIP-3α/CCL20, sCD40L, TNFα, TNFβ
MouseTh1/Th26-Plex PanelIFNγ, IL-4, IL-5, IL-6, IL-12p70, TNF-α
MouseTh1/Th27-Plex PanelIFN-g, IL-1b, IL-2, IL-6, IL-10, IL-12(p70), TNF-α
MouseTh1/Th27-Plex PanelIL-2, IL-4, IL-5, IL-6, IL-9, IL-10, L-13
MouseTh1/Th28-Plex PanelIL-2, IL-4, IL-5, IL-10, IL-12 (p70), GM-CSF, IFN-γ, TNF-α
MouseTh1/Th211-Plex PanelGM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12p70, IL-13, IL-18, TNF-α
MouseTh1/Th220-Plex PanelEotaxin, GROα/KC/CXCL1, GM-CSF, IFNγ, IL-1β, L-2, IL-4, IL-5, IL-6, IL-12p70, IL-13, IL-18, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα
MouseTreg6-Plex PanelIL-9, IL-10, IL-17A, IL-22, IL-23, IL-27
MouseTreg15-Plex PanelIL-10, IL-17A, IL-22, IL-23, IL-27, IL-9, GROα, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, Eotaxin
MouseTreg17-Plex PanelGM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, TNFα
MouseTGF-β3-Plex PanelTGF-β1, TGF-β2, TGF-β3
MouseT Cell15-Plex PanelIL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IFNγ, GM-CSF, Granzyme B, MIP-1β/CCL4, RANTES/CCL5, sCD137/4-1BB/TNFRSF9, sFas, sFasL/TNFRSF6, TNFα
MouseMMPs5-Plex PanelMMP-2, MMP-3, MMP-8, proMMP-9, MMP-10
MouseImmunoglobulin Isotyping6-Plex PanelIgA, IgG1, IgG2α, IgG2b, IgG3, IgM
MouseKidney Injury 5-Plex PanelClusterin, CystatinC, EGF, NGAL/Lipocalin-2, Osteopontin (OPN)
MouseKidney Injury 6-Plex PanelIP-10/CXCL10, KIM-1, Renin, TIMP-1, VEGF-A, β-2-Microglobulin
MouseObesitySingle-Plex PanelAdiponectin
MouseObesity7-Plex PanelIL-6, Insulin, Leptin, MCP-1/CCL2, PAI-1 (total), Resistin, TNFα
MouseObesity7-Plex PanelAdiponectin, IL-6, Leptin, MCP-1/CCL2, PAI-1 (total), Resistin, TNFα
MouseNeuropeptide6-Plex PanelNeurotensin, OrexinA, Oxytocin, SubstanceP, α-MSH, β-Endorphin
MouseDiabetes7-Plex PanelGhrelin, GIP, GLP-1, Insulin, Leptin, PAI-1, Resistin
MouseMetabolic Hormone13-Plex PanelAmylin (total), C-Peptide2, Ghrelin(active)%, GIP (total), GLP-1 (total), IL-6, Insulin, Leptin, MCP-1/CCL2, Pancreatic Polypeptide, PYY (total), Resistin, TNFα
MouseIGFBP6-Plex PanelIGFBP1, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IGFBP7
MousePituitary7-Plex PanelACTH, BDNF, FSH, GH, LH, Prolactin, TSH
MouseBone MetabolismSingle-Plex PanelRANKL
MouseBone Metabolism10-Plex PanelACTH, DKK-1, FGF-23, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteoprotegerin (OPG), Sclerostin (SOST), TNFα
RatCytokine/Chemokine14-Plex PanelIL-1α, IL-1β, IL-10, IL-13, IL-2, IL-4, IL-6, ICAM-1, IFN-γ, GM-CSF, L-Selectin, TIMP-1, TNF-α, VEGF
RatCytokine/Chemokine27-Plex PanelEotaxin, EGF, Fractalkine, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IP-10, GRO/KC, TNFα, G-CSF, GM-CSF, MCP-1, Leptin, LIX, MIP-1α, MIP-2, RANTES, VEGF-A
RatCytokine/Chemokine8-Plex PanelGROα/KC/CINC1/CXCL1, MCP-1, MIP-1α, Rantes, Eotaxin/CCL11, IP-10, MCP-3, MIP-2/Gro-β
RatInflammation9-Plex PanelIL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-13, IFN-γ, TNF-α
RatTh1/Th212-Plex PanelL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, GM-CSF, IFN-γ, TNF-α
RatImmunoglobulin IsotypingSingle-Plex PanelIgE
RatImmunoglobulin Isotyping6-Plex PanelIgA, IgG1, IgG2α, IgG2b, IgG2c, IgM
RatCardiovascular Disease4-Plex PanelAdiponectin, sE-Selectin, sICAM-1, von Willebrand Factor (vWF)
RatCardiovascular Disease4-Plex Panelα-2-Macroglobulin (A2M), α1-Acid Glycoprotein (AGP), FibrinogenΔ, Haptoglobin
RatCardiovascular Disease6-Plex Panelα1-Acid Glycoprotein (AGP), Albumin, CystatinC, EGF, NGAL/Lipocalin-2, β-2-Microglobulin
RatCardiovascular Disease7-Plex PanelCardiac Troponin I (cTnl), Cardiac Troponin T (cTnT), Creatine Kinase Myscle (CKM), FABP3, Follistatin-like Protein1 (FSTL1), Myosin Light Chain3 (MYL3), TIMP-1
RatCardiovascular Disease8-Plex PanelCalbindin, Clusterin, GSTα, IP-10/CXCL10, KIM-1, Osteopontin (OPN), TIMP-1, VEGF-A
RatCardiovascular Disease9-Plex PanelCaveolin-1, Connective Tissue Growth Factor (CTGF), GROα/KC/CINC-1/CXCL1, IL-6, MCP-1/CCL2, PAI-1(total), TIMP-1, TNFα, VEGF-A
RatMetabolic Hormone11-Plex PanelAmylin(active)*, C-Peptide 2, GIP(total), GLP-1(active)*, Ghrelin(active)*, Insulin, Leptin, PP, PYY, Resistin (mouse only), Secretin
RatNeuropeptide6-Plex PanelNeurotensin, OrexinA, Oxytocin, SubstanceP, α-MSH, β-Endorphin
RatBone Metabolism8-Plex Panel (Serum/Plasma)ACTH, DKK1, FGF-23, Insulin, Leptin, Osteoprotegerin (OPG), PTH, Sclerostin (SOST)
RatBone Metabolism10-Plex Panel (Cell culture)ACTH, DKK-1, FGF-23, Insulin, Leptin, Osteocalcin (OC), Osteoprotegerin (OPG), Osteopontin (OPN), PTH, Sclerostin (SOST)
RatKidney Injury 3-Plex PanelAlbumin, Cystatin C, Lipocalin-2/NGAL
RatKidney Injury 6-Plex Panel AClusterin, GSTα, IP-10, KIM-1, OPN – Osteopontin, TIMP-1, VEGF
RatKidney Injury 6-Plex Panel Bα1-Acid Glycoprotein (AGP), Albumin, CystatiC, EGF, NGAL/Lipocalin-2, β-2-Microglobulin
RatLiver Injury5-Plex Panel5NT/CD73, ARG1, GOT1, GSTα, SDH
RatAdiponectinSingle-Plex Panel (Serum/Plasma)Adiponectin
RatStress Hormone3-Plex PanelACTH, Corticosterone, Melatonin
RatThyroid6-Plex PanelCortisol, Estradiol, Progesterone, T3(Total), T4 (Total), Testosterone
RatAdipokine7-Plex Panel (Serum/Plasma)IL-1β, IL-6, Insulin, Leptin, MCP-1/CCL2, PAI-1(total), TNFα
RatAdipocyte7-Plex Panel (Cell culture)Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 (Total), TNFα
RatPituitary7-Plex PanelAdiponectin, IL-1β, IL-6, Leptin, PAI-1 (total), TNFα, MCP-1
NHPCytokine/Chemokine7-Plex PanelBDNF, FGF-basic, PDGF-BB, SCF, VEGF-A, VEGF-D, bNGF
NHPCytokine/Chemokine10-Plex PanelIL-8, IP-10, MCP-1, MIP-11, MIP-1β, Eotaxin, SDF-1, BLC/CXCL13, I-TAC/CXCL11, MIG/CXCL9
NHPCytokine/Chemokine10-Plex PanelGM-CSF, IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A
NHPCytokine/Chemokine23-Plex PanelG-CSF, GM-CSF, IFN-γ, IL-1ra, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12/23 (p40), IL-13, IL-15, IL-17, IL-18, MCP-1, MIP-1α, MIP-1β, sCD40L, TGF-α, TNF-α, VEGF
NHPCytokine/Chemokine24-Plex PanelsCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA
NHPCytokine/Chemokine25-Plex PanelsCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, RANTES/CCL5, TNFβ/LTA
NHPProinflammatory9-Plex PanelIFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12/IL-23p40, TNF-α
NHPT-Cell12-Plex PanelGM-CSF, IFN-γ, IL-2, lL-4, IL-9, IL-10, IL-13, IL-17A, IL-17F, IL-22, MIP-3α, TNF-α
NHPTGF-β3-Plex PanelTGF-β1, TGF-β2, TGF-β3
NHPTH1/TH29-Plex PanelIFN-γ, lL-1β, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, TNF-α
NHPTH179-Plex PanelIFN-γ, lL-1β, IL-2, IL-4, IL-5, IL-8, IL-10, IL-17A, TNF-α
NHPMetabolic Hormone10-Plex PanelAmylin (Active), C-Peptide, Ghrelin (Active), GIP, IL-6, Insulin, Leptin, MCP-1, PP, PYY
NHPPituitary2-Plex PanelBDNF, Prolactin
NHPPituitary7-Plex PanelACTH, AgRP, CNTF, FSH, GH, LH, TSH
NHPDiabetes10-Plex PanelAdipsin, C-peptide, Ghrelin, GIP, GLP-1, Insulin, Leptin, PAI-1, Resistin, Visfatin
PorcineCytokine/Chemokine9-Plex PanelIL-1β、IL-4、IL-6、IL-8、IL-10、IL-12p40、IFNα、IFNγ、TNFα
PorcineCytokine/Chemokine13-Plex PanelGM-CSF, IFNγ, IL-10, IL-12, IL-18, IL-1Ra、IL-1α、IL-1β、IL-2、IL-4、IL-6、IL-8、TNFα
PorcineThyroid6-Plex PanelCortisol, Estradiol, Progesterone, T3(Total), T4 (Total), Testosterone
PorcineTGFβ3-Plex PanelTGFβ1, TGFβ2, TGFβ3
CanineCytokine11-Plex PanelIFNγ, IL-2, IL-6, JIL-8/CXCL8, IL-10, IL-12p40, MCP-1(CCL2), NGFβ, SCF, TNFα, VEGF-A
CanineCytokine13-Plex PanelGM-CSF, IFNγ, IL-10, IL-15, IL-18, IL-2, IL-6, IL-7, IL-8/CXCL8, IP-10/CXCL10, KC-like, MCP-1/CCL2, TNFα
CanineMetabolism8-Plex PanelAmylin (total), Ghrelin (active)%, GIP (total), GLP-1 (active), Insulin, Leptin, Pancreatic Polypeptide, PYY (total)
CaninePituitary7-Plex PanelACTH, BDNF, FSH, GH, LH, Prolactin, TSH
CanineKidney Injury4-Plex PanelClusterin, KIM-1, MCP-1, NGAL
CanineKidney Injury4-Plex Panel (Urine)Albumin, RBP4, TFF3, β-2-Microglobulin
CanineKidney Injury7-Plex Panel (Urine)Clusterin, CystatinC, IL-8/CXCL8, KIM-1, MCP-1/CCL2, NGAL/Lipocalin-2, Osteopontin
CanineThyroid6-Plex PanelCortisol, Estradiol, Progesterone, T3(Total), T4 (Total), Testosterone
CanineTGFβ3-Plex PanelTGFβ1, TGFβ2, TGFβ3
FelineCytokine19-Plex PanelF1t3 Ligand、GM-CSF、GRO/KC、IFNy、IL-12(p40)、IL-13、IL-18、IL-1β、IL-2、IL-4、IL-6、IL-8/CXCL8、MCP-1/CCL2、PDGF-BB、RANTES/CCL5、SCF、SDF-1/CXCL12、sFas、TNFa
FelineMetabolic Hormone8-Plex PanelAmylin (total), Ghrelin(active)%, GIP (total), GLP-1 (total), Insulin, Leptin, Pancreatic Polypeptide, PYY (total)
EquineCytokine23-Plex PanelEotaxin/CCL11、FGF-2、Fractalkine/CX3CL1、G-CSF、GM-CSF、GRO、IFNγ、IL-1α、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8/CXCL8、IL-10、IL-12(p70)、L-13、IL-17A/CTLA8、IL-18、IP-10/CXCL10、MCP-1/CCL22、RANTES/CCL5、TNFα

How Does the Luminex Assay Multiplex Work?

Bead Incubation: Incubate Luminex beads, which are pre-coated with target-specific capture antibodies, in a sample containing various target proteins.

First Wash: Wash off unbound proteins, leaving only the target proteins attached to the beads.

Detection Antibody Incubation: Add biotinylated detection antibodies specific to the target proteins. These antibodies bind to the targets, forming a "sandwich" with the capture antibodies on the beads.

Second Wash: Wash away excess detection antibodies.

Reporter Incubation: Incubate with phycoerythrin (PE)-conjugated streptavidin. The streptavidin-PE binds to the biotinylated detection antibodies, completing the detection complex.

Flow Cytometry: The beads, now with the complete sandwich complex, are passed through a flow cell. The dual laser system excites the beads and the reporter molecules, detecting and quantifying the fluorescent signals.

Data Analysis: The fluorescent output is processed by a high-speed digital signal processor, which analyzes and quantifies the presence and concentration of the targets in the sample.

Luminex Parameter

Parameter Specification
Technology Luminex xMAP technology
Detection Method Fluorescent flow cytometry
Capture Antibodies Highly specific monoclonal antibodies coupled to fluorescently labeled magnetic beads
Signal Amplification Biotin-labeled secondary antibodies combined with SA-PE
Multiplex Capacity Up to 100 analytes (80 on MagPlex)
Dynamic Range ≥3.5 logs
Sensitivity 0.06–1 pg/mL
Read Time 96-well in ~20 min
Sample Volume 6.3–80 µL (assay dependent)
Sample Types Serum, plasma, cell culture supernatants
Plate Format 96-well
Automation Compatible Yes, with front eject option
Operational Validation Calibration, verification, and fluidics
Applications Protein assays

Why Choose the Luminex® 200™ System?

The Luminex® 200™ System stands out as a robust and reliable multiplexing platform, recognized for its exceptional performance with over 9,000 citations in scientific literature. This system is ideal for a broad range of applications, from drug discovery to diagnostics and research. Here’s a detailed look at its applications and benefits:

Drug Discovery and Development

Research Assay Applications

Applications of Luminex Assay

Clinical Diagnostics

Disease Detection: Customized assays can be tailored to detect specific biomarkers for diseases such as cancer, cardiovascular conditions, or infectious diseases.

Patient Monitoring: They can be used to monitor disease progression or treatment response by measuring levels of specific proteins or cytokines.

Research and Development

Biomarker Discovery: Preconfigured assays can be used in research to identify and validate new biomarkers for diseases.

Drug Development: Customized assays are valuable for screening drug candidates and understanding their mechanisms of action.

Immunology

Cytokine Profiling: They allow for the simultaneous measurement of multiple cytokines, helping to understand immune responses and inflammation.

Autoimmune Diseases: Assays can be designed to detect autoantibodies and other markers relevant to autoimmune conditions.

Genomics and Proteomics

Gene Expression Studies: Assays can be used to quantify protein levels in relation to gene expression, providing insights into gene function and regulation.

Protein Interaction Studies: They help in studying protein-protein interactions, which are crucial for understanding cellular processes.

Environmental and Food Safety

Contaminant Detection: Customized assays can detect specific contaminants or toxins in food and environmental samples.

Quality Control: They are used in food and beverage industries to ensure product quality and safety.

Pharmacokinetics and Toxicology

Drug Metabolism: Assays can measure drug levels and metabolites to study pharmacokinetics and drug interactions.

Toxicity Testing: They help in evaluating the potential toxicity of new compounds or chemicals.

Personalized Medicine

Genotype-Phenotype Correlations: Customized assays can be used to tailor treatments based on individual genetic profiles or specific health conditions.

FAQ

What are the best practices for sample preparation in Luminex assays

Sample Collection and Storage

  • Collect samples according to standard protocols and avoid hemolysis, which can interfere with the assay.
  • Determine the maximum storage time for your specific sample type and analyte of interest. General guidelines are provided in product datasheets, but stability should be evaluated for each sample.
  • Store samples at the appropriate temperature (-70°C or below for long-term storage) and avoid repeated freeze-thaw cycles.

Sample Dilution

  • Dilute samples according to the assay protocol. Avoid diluting samples in assay diluents containing heterophilic blocking reagents, as R&D Systems kits are designed to minimize interference without the need for additional blockers.
  • For urine samples, normalize analyte values to creatinine concentration by expressing results as units/mg creatinine.

Special Sample Types

  • For certain sample types, such as serum or plasma, additional preparation steps may be required. Refer to the specific kit protocol for any special instructions.
  • For PBMC samples, proper preparation is critical for reproducible results. Consult the kit protocol and technical support if needed.

Mixing and Vortexing

  • Mix samples thoroughly by vortexing or pipetting up and down before adding to the assay plate. Insufficient mixing can lead to uneven distribution of analytes.
  • Avoid excessive vortexing, which may cause foaming or denaturation of proteins.

By following these best practices for sample collection, storage, dilution, and handling, you can optimize the performance and reliability of your Luminex assays.

What types of analytes can be measured using Luminex multiplex assays?

Luminex multiplex assays can measure a broad range of analytes, including proteins, cytokines, hormones, nucleic acids, and other biomarkers. The specific analytes detectable depend on the assay panel and the capture reagents used. Panels can be customized to target specific analytes of interest based on research or clinical needs.

How should samples be prepared for Luminex multiplex assays?

Sample preparation involves several steps which can vary depending on the assay:

  • Dilution: Samples often need to be diluted to fit within the assay's detection range.
  • Centrifugation: To remove debris and obtain a clear supernatant.
  • Filtration: To eliminate particulate matter that might interfere with the assay.

Always consult the specific assay protocol for precise preparation steps to ensure optimal results.

What is the recommended sample volume?

The required sample volume varies by assay. Generally, a range of 25-100 µL is used, but you should refer to the specific assay protocol for exact requirements. Accurate volume measurement is crucial for reliable results.

What should I do if I encounter low sensitivity or poor assay performance?

If sensitivity or performance issues arise:

  • Check Reagents: Ensure that all reagents are not expired and have been stored under recommended conditions.
  • Review Protocol: Verify that all steps were followed correctly and that reagent concentrations are accurate.
  • Optimize Conditions: Consider optimizing assay conditions such as incubation times or temperatures.
  • Consult Support: If problems persist, consult the troubleshooting section of the assay manual or contact technical support.

How do I handle high background or non-specific signals?

High background or non-specific signals can be managed by:

  • Optimizing Protocols: Adjusting reagent concentrations, incubation times, and washing steps.
  • Using Controls: Incorporating appropriate controls to distinguish specific signals from background noise.
  • Reagent Quality: Ensuring that reagents are fresh and properly handled to minimize non-specific interactions.

What if there is a lot-to-lot variation between assays?

Lot-to-lot variation can be mitigated by:

  • Using Controls: Consistent use of controls to normalize data across different lots.
  • Calibrating Instruments: Regular calibration of the detection instrument to maintain consistency.
  • Consulting Guidelines: Following the manufacturer's guidelines for lot-specific instructions and recommendations.

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