Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of leptin signaling pathway.
(Brennan AM, et al.2019)Leptin is a secreted protein encoded by the ob gene located on human chromosome (7q 31. 3) and exerts its physiological functions by binding to the leptin receptor. Leptin is a pleiotropic hormone that can participate in the proliferative and anti-apoptotic effects of several systems simultaneously. Previous studies have suggested that leptin can reduce appetite and body weight, regulate fat deposition and energy balance, regulate neuroendocrine effects, and participate in the immune response of the body. Recent studies have found that leptin plays a role in promoting tumor cell proliferation and metastasis by mediating certain intracellular signaling pathways.
The leptin signaling pathway and its mediated signaling pathways have a role in promoting tumor cell proliferation and metastasis. With the in-depth study of leptin and its mediated signal transduction pathway, we will explore its intrinsic connection with tumor development and seek effective chemical or genetic drugs to inhibit or cut off the leptin-mediated signaling pathway, which will lay the experimental foundation for tumor prevention and control of tumor development.
Leptin is a hormone produced in fat cells that affects the brain and some other peripheral tissues. It plays a key role in the regulation of endocrine function and control of energy expenditure. In addition to adipose tissue, the leptin signaling pathway has regulatory roles in other tissues including placenta, ovary, skeletal muscle, pituitary and lymphatic tissue. Leptin regulates food intake and also plays a key role in energy expenditure, metabolism, neuroendocrine axis and immune function.
PTP | VPS34 | Vav | CrkII | TRAF6 | MEK6 |
PROLC3 | PI3K | TBK1 | IRF9 | IRSI-4 | p50 |
PDHK | FIP200 | mTOR | AMBRA1 | IRS1 | p53 |
LC3I | ULK | MSK2 | SH2 | p38MAPK | TRAM |
c-Myc | Bax | ISGF3 | RIP1 | MEK3 | TRIF |
ULK1 | Grb2 | CAP | p38 | IRF5 | IRAK1 |
PFK | VDAC | TRAF3 | TRAF5 | Shp-2 | ISRE |
PHGDH | ANT | RIG-1 | Gab-1 | TLR3 | PKR |
Erk1 | LDHA | NFκB | IRS2 | Tak1 | |
Shc | LKB1 | UYRAG | MSK1 | p65 |
We provide Luminex technology for leptin signaling pathway analysis.
Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.
Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing leptin signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.
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