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Introduction

Single-molecule Array (Simoa) technology enables ultra-high sensitivity detection of proteins in body fluids such as serum and plasma, mainly in the direction of oncology, neurology, infectious diseases, immune inflammation, reproduction, ophthalmology, cardiovascular and pharmaceuticals.

The sensitivity of ELISA can generally only reach the pg/mL level, which is often inadequate for the detection of ultra-low-abundance proteins. However, accurate detection of ultra-low-abundance proteins is of great significance for disease mechanism research and prediction of disease occurrence and regression. Along with the development of health industry and precision medicine, the contradiction between the technical bottleneck of traditional methods and the huge market demand is becoming more and more obvious. Simoa technology can solve this problem. The technology is 1000 times more sensitive than ELISA. It brings protein detection technology directly into the era of single molecule, digital detection, and becomes the dominant technology in the field of fg-level ultra-low-abundance protein detection.

Creative Proteomics offers Simoa cytokine and protein assay services. We can achieve:

Advantages of Simoa Assay

Principle of Simoa Analysis Technology

The biological principle of the Simoa assay remains the same as that of the double antibody sandwich ELISA. The Simoa technology differs in that approximately 250,000 capture antibodies are encapsulated on small 2.7 μm magnetic beads. Biotin-labeled detection antibodies and affinity-coupled enzymes and substrates are added to the assay. The individual beads are individually enclosed in 238,000 4.5 μm reaction wells by a layer of oil. Since the reaction system is only 50 femtoliters per well, which is 2 billion times smaller than a conventional ELISA, the catalytic substrate can generate 3,000 fluorescent molecules even if only one molecule is present in the well. The signal can be captured by the CCD camera, and the Poisson distribution theory can be used to calculate the protein concentration value corresponding to the positive fluorescent wells (On Well), realizing the desire of digital single molecule detection.

SiMoA schematic workflow showing stepwise procedures performed to quantify cytokines in single cellsSiMoA schematic workflow showing stepwise procedures performed to quantify cytokines in single cells (Saxena et al., 2018).

Sample Requirements

Samples from humans, mice, rats, primates, pigs, horses, dogs, cats and other species.

Samples can be tested: serum, plasma, cerebrospinal fluid, cell culture supernatant, exosomes, atrial fluid, vitreous humor, tears, sweat, urine, saliva, microscopic cells, individual blastocyst cultures, blood topographies, clots, etc.

Creative Proteomics can provide you with a one-stop solution for cytokine and protein detection and quantification through single molecule array technology. Please contact us if you would like to employ other assay technologies or inquire about this service. We look forward to working with you.

Reference:

  1. Saxena, A., Dagur, P. K., et al (2018). Ultrasensitive Quantification of cytokine proteins in single lymphocytes from human blood following ex-vivo stimulation. Frontiers in immunology, 9, 2462.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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