DNA-PK Signaling Pathway Luminex Multiplex Assay

Introduction to DNA-PK signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of apoptosis signaling pathway.

The DNA-dependent protein kinase (DNA-PK) is a serine/threonine protein kinase composed of a large catalytic subunit (DNA-PKcs) and the Ku70/80 heterodimer. Over the past two decades, significant progress has been made in elucidating the role of DNA-PK in non-homologous end joining (NHE), the major pathway for repair of ionizing radiation-induced DNA double strand breaks in human cells and recently, additional roles for DNA-PK have been reported.

DNA-dependent protein kinase (DNA-PK) is one of the most important enzymes that bind DNA and repair double-strand breaks. This type of repair is essential to produce receptors that can help the immune system defend against invaders. But DNA-PK not only binds DNA, but also binds RNA.

DNA-PK exists in the organelles that make ribosomes. DNA-PK mutations can impair protein translation in red blood cell progenitor cells, which may lead to anemia. And recent studies have found that DNA-PK seems to be related to ribosome assembly.

The DNA-PK signaling pathway is essential for the cell viability of almost all cell lines (including cancer cell lines), and many other proteins involved in the same DNA repair pathway are not essential. Several previous studies have shown that the DNA-PK protein level in ordinary human cell lines is 50 times higher than that in rodent cell lines.

Our detectable targets:

Technology platform:

We provide Luminex technology for DNA-PK signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

DNA-PK is a serine-threonine protein kinase that requires ends of double-stranded DNA or transitions from single-stranded to double-stranded DNA for activity. It is composed of a kinase element known as DNA-PKcs and a DNA binding element known as Ku, which is itself a heterodimer comprising Ku80 and Ku70. Cells lacking DNA-PK activity as a result of a mutation in any of the subunits are radiosensitive to killing and deficient in the rejoining of radiation-induced DNA DSBs. The DNA-dependent protein kinase signaling pathway plays an important role in DNA double-strand break repair and mitosis and other cellular processes.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of DNA-PK signaling pathway detection:

• High-throughput and high-speed: Each microsphere is used as a separate test body, which can perform a large number of biological tests at the same time. It only needs 10~201 samples to test up to 100 indicators at a time, and the fastest can reach 10,000 tests /hour.
• Good repeatability: The Luminex detection platform directly reads the fluorescence value, which is more stable and sensitive: 100 microspheres of each type are detected, and the median value is taken as the result, which is equivalent to 100 repeated detections for each sample.
• Low cost: Simultaneous detection of multiple indicators of a sample can save time, samples, reduce detection costs, and improve analysis efficiency.

Application of our service:

• To study the regulation mechanism of DNA-PK signal pathway in disease
• To study the effect of each virus on DNA-PK signaling pathway
• To study the effects of drugs or therapies on DNA-PK signaling pathways

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing DNA-PK signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

1. Omar Nasser Rahal, Maamoun Fatfat. Chk1 and DNA-PK mediate TPEN-induced DNA damage in a ROS dependent manner in human colon cancer cells. Cancer Biology & Therapy, 2016, 1: 1139-1148.
2. Hill R, Lee PW. The DNA-dependent protein kinase (DNA-PK): More than just a case of making ends meet? Cell cycle, 2010, 9: 3460-3469.
3. Zheng ping Shao, et al. DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis. Nature, 2020.