PAI-1 signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of diabetes signaling pathway.

Plasminogen-Activator-Inhibitor-1(PAI-1) Signaling Pathway Detection Service(Sapru, A., et al. 2019)Plasminogen activator inhibitor-1 (PAI-1) is the main physiological inhibitor of endogenous plasminogen activator (pa) and is an important active substance in the exogenous activation pathway of the fibrinolytic system and the most important regulator of fibrinolytic activity in vivo. It has been associated with a variety of human diseases and is involved in many cardiovascular risk factors.

PAI-1 is a key regulator of fibrinolysis and coagulation and plays an important pathological role in immune and inflammatory responses.PAI-1 functions as an acute-phase protein during acute lung injury (ALI), inflammation and sepsis. The potential role of PAI-1 in the pathogenesis of lung inflammation is evidenced by its upregulation in various experimental models of ALI Evidence. Furthermore, the concentration of PAI-1 in bronchoalveolar lavage (BAL) fluid from ALI patients has been associated with mortality. Although lipopolysaccharide (LPS)-induced neutrophil recruitment and imbalance of local coagulation and fibrinolytic activity via PAI-1 have been reported, the exact mechanism of PAI-1 involvement in pulmonary inflammation has not been fully elucidated.PAI-1 deficiency may exacerbate LPS-induced acute lung injury in mice by downregulating Toll-like receptor 4 (TLR4) negative regulators and enhancing the TLR4 signaling pathway, but PAI-1 deficiency may be associated with a decrease in PAI-1 in mice.

The PAI-1 signaling pathway plays an important role in regulating the LPS-induced inflammatory response in alveolar macrophages.PAI-1 signaling may promote the expression and activation of TLR4-MD-2/MyD88/NF-KB signaling pathway to exaggerate lung inflammation in vitro. Inhibition of PAI-1 may lead to novel therapeutic approaches against bacterial or LPS-induced lung inflammation.PAI-1 knockdown reduced NF-KB p65 expression after LPS stimulation, whereas PAI-1 overexpression showed the opposite result. It indicates that in the LPS-induced inflammatory response, PAI-1 promotes inflammation through the TLR4/NF-KB pathway in vitro, whereas deletion of PAI-1 resulted in decreased TLR4/NF-KB pathway activity and reduced inflammation.

Our detectable targets:

PTPVPS34UYRAGCrkIIIRS1MEK6
ANTPI3KNFκBIRF9IRSI-4p50
LDHAFIP200RIG-1AMBRA1TRAF6p53
LKB1ULKTRAF3p38SH2TRAM
ShcPROLC3CAPRIP1p38MAPKTRIF
Erk1LC3IISGF3TRAF5MEK3IRAK1
PHGDHPDHKMSK2Gab-1IRF5ISRE
PFKGrb2mTORIRS2Shp-2PKR
ULK1BaxTBK1MSK1TLR3 
c-MycVDACVavp65Tak1 

Technology platform:

We provide Luminex technology for PAI-1 signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.

Advantages of PAI-1 signaling pathway detection:

Advantages of PAI-1 signaling pathway detection

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing PAI-1 signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Sapru, A., M.A. Curley, et al. Elevated PAI-1 is associated with poor clinical outcomes in pediatric patients with acute lung injury. Intensive Care Medicine, 2019, 36: 157–163.
  2. Kwak, S.H., X.Q. Wang, et al. Plasminogen activator inhibitor-I potentiates LPS-induced neutrophil activation through a JNK-mediated pathway. Thrombosis and Haemostasis, 2018, 95: 829–835.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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