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Cytokine/Chemokine in Chicken
Cytokines and chemokines are crucial molecular mediators in chickens, serving as the foundation for immune regulation and communication. These small proteins are responsible for coordinating the immune system's response to infections, inflammation, and tissue repair, making them essential biomarkers for understanding avian health. Their significance extends to applications in vaccine development, disease resistance studies, and enhancing overall poultry productivity.
In chickens, cytokines like interleukins (e.g., IL-1, IL-6) and interferons (e.g., IFN-γ) play pivotal roles in activating immune defenses, while chemokines such as CXCL8 direct immune cells to sites of infection or injury. These molecules work in concert to maintain immune balance, ensuring robust protection against pathogens without triggering excessive inflammation.
Understanding cytokine and chemokine dynamics is not only vital for deciphering the complexities of the avian immune system but also for driving innovation in poultry health management. Whether it's fine-tuning vaccines for optimal efficacy or identifying immune-related biomarkers for disease resistance, the study of these proteins opens doors to transformative advancements in poultry science.
Chicken Cytokine/Chemokine Panel at Creative Proteomics
Creative Proteomics offers a comprehensive Chicken Cytokine/Chemokine Panel tailored to meet the needs of researchers studying avian immunology and poultry health. Using advanced Luminex xMAP technology, our service enables the simultaneous detection and quantification of multiple cytokines and chemokines in a single sample.
Our panels are designed to provide flexibility, offering predefined options for commonly studied analytes as well as fully customizable solutions to address specific research goals. With support for various sample types, including serum, plasma, cell culture supernatants, and tissue homogenates, the platform ensures compatibility with diverse experimental requirements.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Chicken
Analytes Detected
| Species | Specification | Protein Targets | Application | Price |
|---|---|---|---|---|
| Chicken | Chicken Cytokine/Chemokine 12-Plex Panel | RANTES, IL-6, IFNγ, MIP-3α, IL-10, IFNα, IL-21, MIP-1β, VEGF, IL-2, IL-16, M-CSF | Suitable for analyzing immune response, inflammation, and cytokine profiles in avian species | +Inquiry |
Sensitivity (Detection Limits)
- RANTES: 5–10 pg/mL
- IL-6: 10–15 pg/mL
- IFNγ: 5–10 pg/mL
- MIP-3α: 6–12 pg/mL
- IL-10: 6–12 pg/mL
- IFNα: 8–14 pg/mL
- IL-21: 7–14 pg/mL
- MIP-1β: 4–9 pg/mL
- VEGF: 10–20 pg/mL
- IL-2: 3–8 pg/mL
- IL-16: 5–10 pg/mL
- M-CSF: 10–20 pg/mL
Advantages of Chicken Cytokine/Chemokine Luminex Assay
- Multiplex Capability: Detect up to 50 cytokines and chemokines in a single sample, saving time and sample volume.
- High Sensitivity: Detects cytokines and chemokines at picogram (pg/mL) levels, ensuring accurate measurement of low-abundance analytes.
- Wide Dynamic Range: Accurate quantification across a broad concentration spectrum, from low to high levels.
- Minimal Sample Volume: Requires only small sample volumes, ideal for limited or precious samples.
- Customizable Panels: Tailor the assay to suit specific research needs with flexible panel options.
- Reliable Data: Provides reproducible, high-quality results for consistent and reliable analysis.
- Fast Turnaround Time: Quick results, enabling efficient data collection and analysis.
- Non-radioactive: Safe, non-radioactive detection, ensuring a safer laboratory environment.
- Comprehensive Data Analysis: Includes statistical analysis, graphs, and report generation for easy result interpretation.
- Flexible Sample Types: Compatible with serum, plasma, tissue homogenates, and cell culture supernatants.
Sample Requirements for Chicken Cytokine/Chemokine Luminex Panel
| Sample Type | Required Volume | Sample Preparation | Storage Conditions |
|---|---|---|---|
| Serum | 100 µL | Centrifuge blood to separate serum. Avoid hemolysis. | Store at -80°C for long-term storage. |
| Plasma | 100 µL | Centrifuge blood to separate plasma. Use EDTA or heparin as an anticoagulant. | Store at -80°C for long-term storage. |
| Cell Culture Supernatant | 100 µL | Collect media from cultured cells. Ensure no contamination with cellular debris. | Store at -80°C for long-term storage. |
| Tissue Homogenates | 100 µL | Homogenize tissue in appropriate buffer, avoiding contamination. | Store at -80°C for long-term storage. |
Application of Chicken Cytokine/Chemokine Panel
- Immune Response Monitoring: Assess immune activation and regulation during infections or vaccinations.
- Inflammation Research: Study the inflammatory response in poultry, providing insights into disease progression.
- Vaccine Development: Evaluate immune responses to different vaccine candidates and formulations.
- Disease Resistance Studies: Identify cytokine and chemokine profiles associated with resistance or susceptibility to specific pathogens.
- Poultry Health Management: Monitor and improve immune health in poultry production systems.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| RANTES | A chemokine involved in immune cell recruitment and inflammation; plays a key role in immune response and inflammatory diseases. |
| IL-6 | A cytokine that regulates immune responses, inflammation, and hematopoiesis; linked to chronic inflammatory diseases and immune system regulation. |
| IFNγ | A cytokine that activates immune cells, particularly in response to infections; critical in host defense and immune modulation. |
| MIP-3α | A chemokine that attracts immune cells to sites of infection and inflammation, contributing to the immune response. |
| IL-10 | An anti-inflammatory cytokine that inhibits immune cell activation and suppresses inflammation, balancing immune responses. |
| IFNα | A type I interferon involved in antiviral defense and immune regulation, essential for innate immune responses. |
| IL-21 | A cytokine that plays a role in the regulation of immune cell development and function, especially in T cells and B cells. |
| MIP-1β | A chemokine that regulates the recruitment of immune cells to sites of inflammation and infection. |
| VEGF | A growth factor that promotes angiogenesis, supporting tissue repair and immune response in inflammatory conditions. |
| IL-2 | A cytokine essential for T cell proliferation, differentiation, and activation, influencing immune responses. |
| IL-16 | A cytokine that regulates immune cell recruitment and activation, particularly in allergic and inflammatory responses. |
| M-CSF | A growth factor that stimulates the production of monocytes and macrophages, key players in immune responses and inflammation. |
What is the best way to collect blood samples from chickens?
Blood should be collected from the wing vein or jugular vein using sterile techniques. A typical collection volume is 0.5 to 1 mL. Use appropriate tubes for serum or plasma, depending on your analysis needs.
What anticoagulants should I use when collecting plasma samples?
EDTA or heparin are the recommended anticoagulants for plasma collection. EDTA is commonly preferred for cytokine/chemokine studies.
How should serum samples be processed after collection?
Allow the blood to clot for 30-60 minutes at room temperature. Centrifuge at 1000-2000 x g for 10-15 minutes at 4°C. Transfer the serum to a new tube and store at -80°C for long-term storage.
How should plasma samples be processed and stored?
After plasma collection, centrifuge at 1000-2000 x g for 10-15 minutes at 4°C. Transfer plasma into a clean tube and store at -80°C. Avoid repeated freeze-thaw cycles.
What is the recommended procedure for collecting and preparing cell culture supernatants for analysis?
Collect the supernatant after cell culture, centrifuge to remove debris, and transfer the clear supernatant into a new sterile tube. Store at -80°C.
How should tissue samples be prepared for analysis?
Homogenize tissue in a suitable lysis buffer, centrifuge to remove debris, and transfer the supernatant into a clean tube. Store at -80°C.
How long can the samples be stored before analysis?
Serum and plasma should be stored at -80°C for long-term storage. Cell culture supernatants and tissue homogenates should also be stored at -80°C. Avoid storing at temperatures higher than -20°C for prolonged periods.
How can I avoid contamination or degradation of cytokines and chemokines during sample collection?
Use sterile collection equipment, process samples quickly after collection, and store at appropriate temperatures. Avoid repeated freeze-thaw cycles and contamination.
What is the minimum sample volume required for analysis?
The minimum sample volume required is 100 µL for serum, plasma, cell culture supernatants, and tissue homogenates.
Can I use samples that have been stored in non-ideal conditions (e.g., at -4°C or room temperature)?
Samples should ideally be stored at -80°C or -20°C. Storing at room temperature or -4°C may degrade cytokines and chemokines, affecting results.
Are there any special considerations when handling samples from different chicken breeds or ages?
Cytokine/chemokine levels may vary based on age or immune status. If studying specific age groups or immune conditions, consider discussing potential adjustments with our team.
