- Services Overview
- Analytes Details
- FAQ
What is Mouse Sepsis?
Mouse sepsis occurs when harmful bacteria or other pathogens enter the bloodstream, triggering an immune response that causes widespread inflammation. This inflammation can lead to tissue damage, organ failure, and, if left untreated, death. Mice with sepsis may experience symptoms such as fever, rapid heart rate, and low blood pressure, but because mice cannot express their discomfort like humans, identifying sepsis requires careful monitoring by researchers.
Sepsis in mice can be caused by various bacteria, with common culprits including Escherichia coli (E. coli), Staphylococcus aureus, and Pseudomonas aeruginosa. These bacteria can enter the body through open wounds, infected catheters, or the gut, especially in immunocompromised mice. The infection then spreads through the bloodstream, triggering the sepsis process.
How Sepsis Affects Mice
When sepsis occurs, the immune system attempts to fight off the infection by releasing chemicals that promote inflammation. However, in some cases, the body's response to the infection becomes excessive, leading to widespread tissue damage. This uncontrolled immune response can make it harder for the mouse to recover, and in severe cases, it can result in organ failure and death.
Mice with sepsis often show noticeable changes in behavior and health, including reduced activity, labored breathing, decreased appetite, and changes in the appearance of their fur. Their abdomen or limbs may become swollen, and they may appear lethargic or weak. These symptoms serve as key indicators for researchers who are studying sepsis in mouse models.
Why Study Mouse Sepsis?
Mice are widely used in biomedical research because they share many biological similarities with humans, making them valuable models for studying diseases like sepsis. By understanding how sepsis develops and progresses in mice, researchers can gain important insights into how it affects humans as well. This knowledge is crucial for discovering new treatments, therapies, and preventive measures for sepsis in humans.
Additionally, studying mouse sepsis helps scientists understand how the immune system responds to infections. Researchers can test various treatments in mice to observe their effects on the immune system and overall health. These studies help inform the development of drugs, vaccines, and other interventions aimed at improving outcomes for both animals and humans affected by sepsis.
Mouse Sepsis Panel at Creative Proteomics
Mouse Sepsis Panel can help researchers study the complex immune responses triggered by sepsis in mouse models, providing detailed analysis of key cytokines, chemokines and other biomarkers involved in the sepsis process. Our service leverages the advanced Luminex xMAP technology, which allows for the simultaneous detection of multiple analytes from a single sample, significantly improving efficiency and throughput.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Mouse | Mouse Sepsis 6-plex Panel | CCL3/MIP-1 alpha, CCL4/MIP-1 beta, Granzyme B, IL-1 alpha/IL-1F1, MMP-8, Resistin | Suitable for analyzing inflammatory responses, immune activation, and tissue damage in sepsis models. | +Inquiry |
Sensitivity (Detection Limits)
- CCL3/MIP-1 alpha: 0.1–100 pg/mL
- CCL4/MIP-1 beta: 0.1–100 pg/mL
- Granzyme B: 0.2–200 pg/mL
- IL-1 alpha/IL-1F1: 0.1–100 pg/mL
- MMP-8: 0.5–500 pg/mL
- Resistin: 0.5–500 pg/mL
Advantages of the Mouse Sepsis Luminex Assay
- Multiplex Detection: The Luminex xMAP technology enables the simultaneous detection of multiple cytokines and biomarkers in a single sample, reducing sample volume and cost.
- High Sensitivity and Precision: The assay ensures reliable results, even for low-abundance cytokines, essential for studying sepsis where many biomarkers are present at low levels.
- Comprehensive Panel of Biomarkers: The Mouse Sepsis Panel includes key cytokines (TNF-α, IL-1β, IL-6, IL-10) and chemokines (e.g., MCP-1), offering a broad view of the immune response in sepsis.
- Rapid and Efficient: Luminex xMAP technology allows for high-throughput analysis, enabling the processing of large numbers of samples quickly.
- Customizable Panels: Panels can be tailored to specific research needs, allowing flexibility for different sepsis models.
- Reliable Data for Translational Research: The assay provides valuable insights into sepsis progression, aiding the development of diagnostic markers and therapies.
Sample Requirements for Mouse Sepsis Luminex Panel
| Sample Type | Required Volume | Collection Method | Storage/Handling Instructions |
|---|---|---|---|
| Plasma | 50 µL (minimum) | Collected via cardiac puncture or blood collection tubes | Centrifuge to separate plasma from cells. Store at -80°C |
| Serum | 50 µL (minimum) | Collected using serum separator tubes | Allow blood to clot, then centrifuge. Store at -80°C |
| Whole Blood | 100 µL (minimum) | Collected directly in EDTA or heparin tubes | Keep on ice; process within 1 hour or store at -80°C |
| Peritoneal Lavage Fluid | 100 µL (minimum) | Collected via intraperitoneal lavage | Keep on ice and process immediately or store at -80°C |
| Lung Tissue | 50 mg (minimum) | Homogenized tissue from the lung | Snap freeze in liquid nitrogen, store at -80°C |
| Liver Tissue | 50 mg (minimum) | Homogenized liver tissue | Snap freeze in liquid nitrogen, store at -80°C |
| Kidney Tissue | 50 mg (minimum) | Homogenized kidney tissue | Snap freeze in liquid nitrogen, store at -80°C |
Application of Mouse Sepsis Panel
- Sepsis Pathogenesis Research
The panel helps investigate the mechanisms of sepsis by analyzing changes in inflammatory markers, identifying cytokines that contribute to disease progression, and understanding immune dysregulation and tissue damage.
- Immune Response Profiling
It is used to profile the immune response in sepsis models, measuring key markers like TNF-α, IL-1β, IL-6, and IL-10, to assess both pro-inflammatory and anti-inflammatory responses.
- Therapeutic Development and Evaluation
The panel is valuable for evaluating experimental drugs or biologics by monitoring their effect on inflammatory biomarkers, helping determine their potential to mitigate sepsis.
- Biomarker Discovery
The panel aids in identifying new biomarkers for early diagnosis and prognosis, which can contribute to personalized medicine approaches.
- Tissue-Specific Analysis
It allows for tissue-specific analysis, studying immune responses in organs like the lung, liver, and kidney, to understand how sepsis impacts different tissues.
- Sepsis Subtypes and Complications
The panel helps explore sepsis subtypes, such as septic shock and polymicrobial sepsis, revealing distinct cytokine patterns for more targeted treatments.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| CCL3/MIP-1 alpha | A pro-inflammatory chemokine involved in macrophage activation and recruitment during sepsis. |
| CCL4/MIP-1 beta | Works alongside CCL3 to recruit monocytes and macrophages to infection sites in sepsis. |
| Granzyme B | A serine protease that induces apoptosis in infected or damaged cells, critical for immune defense in sepsis. |
| IL-1 alpha/IL-1F1 | A cytokine regulating inflammation, fever, and neutrophil activation during the early immune response in sepsis. |
| MMP-8 | A matrix metalloproteinase involved in neutrophil migration, tissue remodeling, and repair processes in sepsis. |
| Resistin | An adipokine linked to systemic inflammation, often elevated in sepsis and associated with poor outcomes. |
How do I determine the appropriate number of replicates for my experiment?
The number of replicates depends on your study's design and statistical requirements. For exploratory studies, triplicates are generally sufficient to ensure data reliability. However, for studies aiming at publication or clinical relevance, we recommend including biological and technical replicates to account for sample variability and assay precision. Consult with our experts to tailor the design to your specific needs.
Are there any special considerations for using whole blood in the panel?
Yes, whole blood samples must be processed within one hour of collection to prevent degradation of biomarkers. Use EDTA or heparin tubes for collection and keep the samples on ice until processing. If immediate processing is not possible, separate plasma from whole blood and store at -80°C to ensure analyte stability.
How sensitive is the Mouse Sepsis Panel for detecting low-abundance biomarkers?
The Mouse Sepsis Panel is highly sensitive, with detection limits in the picogram per milliliter range for most analytes. This allows for accurate quantification of low-abundance cytokines and chemokines, which are critical in early-stage sepsis studies. For detailed sensitivity information, refer to the detection limits provided for each analyte or contact our team for technical specifications.
How do I ensure consistency in sample collection for longitudinal studies?
For longitudinal studies, standardize the collection protocol across all time points. Use the same collection tubes, anticoagulants, and handling methods to minimize variability. Additionally, process and store all samples under identical conditions. Proper documentation and quality control during sample handling are essential to ensure consistency.
What quality controls are included in the assay?
Each assay run includes internal controls, such as standard curves and positive/negative controls, to ensure accuracy and reliability. Additionally, duplicate or triplicate wells for samples and standards are recommended to minimize technical variability. All data undergo rigorous quality checks before reporting.
