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Protease in Mouse
Proteases are enzymes that catalyze the breakdown of proteins by hydrolyzing peptide bonds. In mouse models, proteases play essential roles in various biological processes, and their activities are tightly regulated in both health and disease states. They are involved in critical functions such as immune response modulation, tissue remodeling, and disease progression. Key roles of proteases in mice include:
- Tissue Remodeling and Repair: Matrix metalloproteinases (MMPs) degrade extracellular matrix components, crucial for tissue remodeling during wound healing, tumor metastasis, and fibrosis. MMP-2 and MMP-9 are key players in collagen and elastin degradation.
- Immune Regulation and Inflammation: Proteases such as cathepsins and elastase regulate immune responses by activating pro-inflammatory cytokines and modulating immune cell function. Caspases, involved in apoptosis, also influence immune responses during infections or autoimmune diseases.
- Neurological Disorders: Proteases like caspase-3 and cathepsin D are implicated in neurodegenerative diseases in mice, such as Alzheimer's and Parkinson's. These enzymes contribute to neuronal damage and inflammation by processing neurotoxic proteins.
- Tumorigenesis and Metastasis: Proteases, including plasminogen activators and MMPs, promote cancer progression and metastasis by degrading the extracellular matrix and facilitating cancer cell migration.
- Angiogenesis and Vascular Remodeling: Proteases like plasminogen activators regulate angiogenesis by breaking down extracellular matrix components, allowing endothelial cells to migrate and form new blood vessels.
Understanding how proteases function in mice is vital for a comprehensive approach to disease modeling, drug discovery, and therapeutic development. Their ability to influence cell signaling, immune modulation, and tissue homeostasis makes them a critical area of study in both fundamental biology and translational research.
Mouse Protease Panel at Creative Proteomics
The Mouse Protease Panel at Creative Proteomics is designed to enable the simultaneous measurement of multiple proteases involved in key biological processes. Using Luminex xMAP Technology, we offer a multiplexed assay that allows for the detection and quantification of several proteases in a single sample, streamlining the research process and providing comprehensive data from a minimal amount of biological material.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Application | Price |
|---|---|---|---|---|
| Mouse | Mouse Protease 5-plex Panel | MMP-2, MMP-3, MMP-8, MMP-12, Proprotein Convertase 9/PCSK9 | Suitable for studying protease activity related to tissue remodeling, inflammation, cancer progression, and neurodegenerative diseases. | +Inquiry |
Sensitivity (Detection Limits)
- MMP-2: 5–10 pg/mL
- MMP-3: 10–15 pg/mL
- MMP-8: 5–10 pg/mL
- MMP-12: 8–15 pg/mL
- Proprotein Convertase 9/PCSK9: 10–20 pg/mL
Advantages of Mouse Protease Luminex Assay
- High Throughput and Multiplexing: The Luminex xMAP platform allows for the simultaneous analysis of up to 100 analytes in a single sample. This multiplexing capability not only saves time but also reduces the number of individual assays required, thus cutting costs and minimizing biological sample usage.
- Sensitivity and Precision: The Mouse Protease Panel uses highly sensitive detection methods, allowing the identification of proteases at picogram-to-nanogram levels. This is essential for studying low-abundance proteases or analyzing samples with limited volumes.
- Versatility and Customization: The ability to customize the panel to target specific proteases or subsets of proteases enables researchers to tailor the assay to their study's exact needs. This flexibility is essential for both basic research and therapeutic development.
- Reliable Data: Luminex technology is renowned for its accuracy and reproducibility. By leveraging precise bead-based assays, we ensure the results are consistent, reducing experimental variability and increasing confidence in data interpretation.
- Cost-Effective Solution: By reducing the need for multiple assays and optimizing sample usage, the Luminex-based protease assay offers a more cost-effective solution without sacrificing data quality or sensitivity.
Sample Requirements for Mouse Protease Luminex Panel
| Sample Type | Minimum Volume | Storage Condition | Notes |
|---|---|---|---|
| Whole Blood | 1-2 mL | Collect in EDTA or heparin tubes | Preferably collect early in the morning to minimize cytokine fluctuations. |
| Serum | 1 mL | Store at -20°C or lower | Avoid hemolysis to prevent assay interference. |
| Plasma | 1 mL | Store at -20°C or lower | Centrifuge within 1 hour of collection to avoid sample degradation. |
| Tissue Homogenate | 100-200 mg | Store at -80°C | Process immediately post-dissection to prevent analyte degradation. |
| Cerebrospinal Fluid (CSF) | 0.5 mL | Store at -80°C | Collect using sterile techniques to avoid contamination. |
| Bronchoalveolar Lavage Fluid (BALF) | 1 mL | Store at -80°C | Process promptly after collection, typically used for pulmonary studies. |
| Urine | 2 mL | Store at -20°C or lower | Avoid contamination and sample degradation. |
Application of Mouse Protease Panel
- Disease Mechanism Studies
Understanding the role of proteases in diseases like cancer, fibrosis, and neurodegenerative disorders can lead to better therapeutic targets and strategies. Our panel allows researchers to study how protease activity changes in response to disease progression.
- Biomarker Discovery
Proteases and their inhibitors are often key biomarkers in various diseases. By profiling protease activity in mouse models, researchers can identify novel biomarkers for early detection and monitoring disease progression.
- Drug Discovery and Screening
Protease inhibitors have become a key area of therapeutic development. Our panel can be used in drug screening efforts to identify potential protease inhibitors and test their effects in vivo, providing valuable data for pharmaceutical research.
- Immunological Studies
Proteases regulate immune responses through the activation and deactivation of immune mediators. Understanding their role in immune signaling pathways can improve our knowledge of immune modulation, especially in the context of autoimmune diseases or inflammation.
- Cancer Research
Many cancer cells secrete proteases to aid in tissue invasion and metastasis. Profiling protease activity in tumor models allows researchers to explore the dynamics of tumor growth and metastasis, which is critical for developing new cancer treatments.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| MMP-2 | Enzyme that breaks down extracellular matrix components, involved in tissue remodeling and inflammation. |
| MMP-3 | Protease that degrades extracellular matrix proteins, plays a role in inflammation and tissue damage. |
| MMP-8 | Collagenase involved in inflammation and tissue destruction, particularly in neuroinflammatory conditions. |
| MMP-12 | Degrades elastin and matrix components, associated with chronic inflammation and neurodegenerative diseases. |
| PCSK9 | Regulates cholesterol metabolism and LDL receptor degradation, implicated in inflammation and neurodegeneration. |
How sensitive is the Mouse Protease Panel?
The mouse protease panel is highly sensitive, with detection limits in the picogram-to-nanogram range for each of the proteases. For example, MMP-2, MMP-8, and other proteases have detection limits as low as 5-10 pg/mL, enabling detection of low-abundance proteases in biological samples. This makes it ideal for studying subtle changes in protease activity, especially in disease models where the protease levels might be low.
How does the Luminex-based assay compare to traditional protease assays?
The Luminex-based multiplex assay offers several advantages over traditional methods. Traditional assays often measure only one protease at a time, which can be time-consuming and require more sample material. The Luminex xMAP technology enables the simultaneous detection of multiple proteases in a single sample, increasing throughput and efficiency. Additionally, Luminex assays are highly sensitive, precise, and cost-effective, making them ideal for high-volume or large-scale studies.
How do I ensure my sample is suitable for the Mouse Protease Panel assay?
To ensure that your sample is suitable for the mouse protease panel assay, it is important to follow the sample collection, processing, and storage instructions carefully. This includes collecting blood in EDTA or heparin tubes, promptly processing plasma or serum, and storing all samples at the appropriate temperatures (usually -80°C) to preserve protease activity. Avoiding hemolysis, contamination, and delays in sample processing is essential for achieving accurate and reliable results.
Can the Mouse Protease Panel be used for longitudinal studies?
Yes, the mouse protease panel is well-suited for longitudinal studies where the activity of specific proteases is monitored over time. By collecting samples at multiple time points, researchers can track changes in protease activity related to disease progression, treatment efficacy, or other experimental conditions. The multiplex nature of the assay also makes it possible to measure multiple proteases in a single sample, streamlining longitudinal data collection.
How do I interpret changes in protease activity in my experimental conditions?
Interpreting changes in protease activity involves comparing protease levels in different experimental conditions. For example, a significant increase in MMP activity may suggest tissue remodeling or inflammation, whereas a decrease could indicate the inhibition of protease activity through therapeutic intervention. In disease models like cancer, changes in MMP and PCSK9 levels can reveal insights into tumor progression, invasion, or metastasis. It is important to compare your findings to appropriate controls to accurately interpret the data.
