Home
Multiplex Assay Kits

Multiplex Assay Kits

More insights. One well.

Magnetic, bead-based multiplex assay kits for multi-analyte quantification across key pathways and species—built to accelerate confident decisions.

Get a Quote

Run more markers with less sample. Our Magnetic Multiplex Assay Kits quantify multiple targets in a single well using xMAP-compatible, bead-based immunoassay technology—ideal for discovery, pathway mapping, and translational research.

How to Pick Your Kit (3 steps)

1. Choose Species

Human, Mouse, Rat, Non-human Primate, Chicken (Gallus), etc.

2. Choose Category

Cytokine subclasses: Th1 / Th2 / Th17 Cytokine
Chemokine, Growth Factor, Hormone/Endocrine, Neurotransmitter, Neuropeptide, Neurodegeneration, Oxidative/Metabolic
If a panel spans multiple families with no clear dominance, we use Multi-Analyte.

3. Choose N-plex

Select the multiplex size that balances coverage and throughput. You can compare kits or request a custom configuration.

Category Guide (at a glance)

Th1 Cytokine – Typically IFNγ, IL-2, TNFα, IL-12 (p70/p40).
Th2 Cytokine – Typically IL-4, IL-5, IL-13, IL-9, IL-10.
Th17 Cytokine – Typically IL-17A/F, IL-21, IL-22, IL-23, IL-6.
Chemokine – CCL/CXCL families (e.g., MCP-1/CCL2, RANTES/CCL5, IP-10/CXCL10).
Growth Factor – VEGF, BDNF, NGF, EGF, FGF/PDGF families, GM-CSF/G-CSF/M-CSF.
Hormone/Endocrine – ACTH, Cortisol, OT, TSH/FSH/LH, E2, T, P4, GH, PRL, Insulin, IGF, etc.
Neurotransmitter / Neuropeptide – 5-HT/Serotonin, DA/Dopamine, GABA, Glutamate; NPY, VIP, SP, CGRP.
Neurodegeneration – Aβ1-40/1-42, Tau, NfL/NEFL, GFAP.
Oxidative/Metabolic – 5-HIAA, MDA, Trp, SOD, CAT, GSH/GSSG, MPO.
Multi-Analyte – Only when a kit is truly mixed with no dominant family.

Choose Your Kit

Why Choose These Kits

Low sample input, high data yield — Quantify many markers from a single well (low-µL inputs), avoiding sample splitting and repeated freeze–thaws.
Analytical sensitivity & range — pg/mL-level LODs (target-dependent) with broad dynamic ranges (≥3–4 logs) using 4/5-parameter logistic (4PL/5PL) curve fits.
Precision you can reproduce — Typical intra-assay CV ≤10–15% and inter-assay CV ≤15–20% (see kit datasheet for target-level values).
Linearity & recovery — Dilution linearity and spike-and-recovery commonly within 80–120%, enabling confident comparability across matrices and runs.
Controlled multiplexing — Bead coupling and cross-reactivity are qualified in multiplex; interference checks minimize analyte crosstalk in high-plex formats.
Lot-to-lot continuity — Reference controls with predefined acceptance windows; each lot is released against QC criteria (CoA available where applicable).
Magnetic workflow, automation-ready — Magnet-based washes reduce manual variability and hands-on time; compatible with 96-well magnets and liquid handlers.
Throughput options — Multiple N-plex sizes to right-size coverage vs. capacity; per-plate event targets and acquisition settings are provided.
Matrix guidance — Serum, plasma, and cell culture supernatant commonly supported; datasheets specify recommended volumes, dilutions, and matrix notes.
Instrument compatibility — Designed for xMAP-compatible readers; bead region maps and acquisition parameters supplied for straightforward setup.

How Multiplex Assay Kit Works?

Setup & Run Time for Multiplex Assay Kits

Plan low-µL sample inputs and appropriate dilutions based on expected concentrations and plex size. A same-day incubate–wash–detect–read sequence is typical. Use blanks and QC controls; include a standard curve spanning the working range.

Step-by-Step Magnetic Multiplex Assay Workflow

1. Capture — Add sample to a color-coded magnetic bead mix; each bead population carries a capture antibody for one target.

2. Detect — Add biotinylated detection antibodies to form sandwiches; add reporter to generate quantitative fluorescence.

3. Readout — Acquire on an xMAP-compatible instrument. The system resolves bead regions (which target) and reporter intensity (how much), then fits a logistic curve to report concentrations.

Interpreting Results — Data Analysis & QC (Standard Curves, LOD/LLOQ, Precision)

Curve fitting — Fit 4PL or 5PL; use appropriate weighting (e.g., 1/Y²) for wide ranges. Inspect residual plots rather than R² alone.
Reportable range — Define from back-calculated standard recoveries (typically 80–120%). Flag results <LLOQ or >ULOQ; avoid extrapolation.
Replicates & precision — Accept a result when replicate CV ≤15–20% (target-dependent). Re-run or dilute if CV exceeds limits.
Blank & background — Subtract blanks consistently; investigate drift if blanks trend upward across the plate.

Dilution factor & units — Apply dilution factors to back-calculated concentrations; report in consistent units (e.g., pg/mL).
Controls & trend — Track plate controls over time (e.g., Levey–Jennings charts) to monitor drift and lot-to-lot continuity.
Matrix effects — Confirm with spike-and-recovery and dilution linearity in your matrix before large runs; adjust diluent or dilution folds accordingly.

Troubleshooting Magnetic Bead Multiplex Assays (Symptom → Cause → Fix)

Low signal → Under-incubation, target below range, reagent degradation→ Extend incubation, verify reconstitution/expiry, decrease dilution or concentrate sample.
High background → Incomplete washes, carryover, non-specific binding→ Increase wash stringency/volume, check sealing and tips, optimize blocking/diluent.
Poor curve fit / compressed high end → Pipetting error, wrong model/weighting, hook effect→ Remake standards, switch 4PL↔5PL or weighting, dilute high samples to remove hook.
Well-to-well variability → Inconsistent mixing, bead settling/aggregation, bubbles→ Mix beads before/throughout use, avoid long magnet dwell, remove bubbles.

Matrix interference → Viscous/hemolyzed/lipemic samples, wrong diluent→ Use matrix-compatible diluent, increase dilution fold, confirm with spike-and-recovery.
Drift across plate / edge effects → Timing/temperature gradients, evaporation→ Standardize incubation timing, maintain temperature, use plate sealers, randomize layout.
Low bead count / region dropout → Magnet alignment, too few events→ Check magnet/plate alignment, increase events per bead, verify region map.

Specifications (typical; target-dependent)

Sample types

Commonly serum, plasma, and cell-culture supernatant; other matrices can be evaluated as needed.

Sample input

Low-volume setup with defined dilution practices to protect limited material.

Assay time

Streamlined magnetic protocol suitable for medium-to-high throughput.

Dynamic range & sensitivity

Broad working ranges with pg/mL-level sensitivity depending on target and matrix.

Precision

Intra-/inter-assay precision monitored across plates and lots; acceptance windows defined before release.

Readout

Compatible with standard xMAP instruments; routine acquisition settings apply.

Performance Snapshots

Standard curves: Representative 4PL/5PL fits demonstrating broad linearity across the working range.
Sensitivity: Target-dependent limits of detection/quantitation, established using validated calibrators and matrix-matched conditions where applicable.
Reproducibility: Representative intra-/inter-assay precision data generated across multiple plates and lots under routine operating conditions.
Recovery & linearity: Typical spike-and-recovery and dilution linearity studies available; matrices and dilution factors are detailed per kit.
Interference control: Bead coupling and cross-reactivity assessments performed in multiplex formats to minimize analyte crosstalk.

FAQs for Multiplex Assay Kits

How do I decide between different plex sizes?

Start from your required analytes, then select the smallest plex size that includes them. Larger plexes expand coverage but may require more careful optimization of dilutions to ensure all targets fall within the quantifiable range.

Can I customize the analyte panel?

Yes. If the stocked kits do not fully match your research needs, you can request a custom configuration. Common approaches include adding or removing certain analytes, or tailoring a panel to a specific pathway or disease area.

Which sample matrices are recommended?

Serum, plasma, and cell culture supernatant are the most commonly validated matrices. For other sample types (e.g., CSF, BAL fluid, tissue lysates), we recommend pilot tests with dilution series and spike-and-recovery checks to confirm performance.

How much sample volume is required per well?

The assay is designed for low-volume inputs, typically in the µL range per well. Actual volume depends on plex size and instrument settings, but most studies can be run with limited material, making the kits suitable for precious clinical or preclinical samples.

What controls should I include in my run?

Always include blanks, a full standard curve, and at least one quality-control sample or calibrator across plates. This ensures consistent quantification and helps monitor assay drift over time.

How reproducible are results across different lots?

Each lot is released only after meeting predefined criteria for standard curve performance, precision, and bead coupling. Certificates of Analysis (CoA) are available where applicable to document lot performance.

Can I automate the workflow?

Yes. The magnetic bead format is compatible with 96-well magnets and most liquid-handling systems. Many labs integrate these kits into semi-automated or fully automated immunoassay workflows.

What is the expected lifetime of reconstituted reagents?

Once reconstituted, reagents should be used promptly and stored according to handling instructions. Stability after reconstitution is limited, so avoid repeated freeze–thaw cycles to maintain performance.

Are results comparable across species panels?

Panels are species-specific, but results can often be compared in relative terms across species. However, absolute values may differ due to antibody affinities and calibrator standards. For true cross-species comparisons, plan parallel runs with harmonized assay conditions.

How should I analyze data from high-plex panels?

Use curve-fitting software capable of 4PL or 5PL logistic regression with appropriate weighting. Apply QC rules (CV limits, recovery ranges) consistently, and avoid extrapolating beyond the reportable range.