PDK-1 Signaling Pathway Luminex Multiplex Assay

PDK-1 signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of PDK-1 signaling pathway.

Phosphoinositide-Dependent Protein Kinase-1 (PDK-1) is a protein encoded by the PDK1 gene in the human body. It involves the development and progression of melanoma. PDK-1 is the main kinase, it is essential for the activation of AKT/PKB and many other AGC kinases (including PKC, SGK, S6K). The important role of PDK-1 lies in the signal transduction pathway activated by several growth factors and hormones.

PDK1 acts as a PDK1 inhibitor. PDK1 can regulate protein kinase B (PKB/Akt), serum, p70 ribosomal S6 kinase (S6K), glucocorticoid-inducible kinase (SGK) and protein kinase C (PKC) in the AGC kinase family Important protein kinases.

PDK1 can participate in the activation of the PI3K-Akt signaling pathway by activating Akt, and promote the occurrence, development, and invasion and metastasis of tumors. At present, PDK1 has been found to be involved in head and neck tumors, multiple myeloma, pancreatic cancer, esophageal cancer, colon cancer, etc. It is highly expressed in a variety of malignant tumors. Inhibition of PDK1 overexpression can find a new breakthrough point for the treatment of malignant tumors. At present, a variety of PDK1 inhibitors have been put into production and have played an anti-tumor effect.

PDK1 activates many AGC kinases in vitro through phosphorylation of T-loop residues, and mediates the activation of PKB, p70 S6 kinase and p90 Rsk in vivo. PDK1 can act as a downstream effector in PI3K signaling and participate in the regulation of neutrophil migration.

Our detectable targets:

Technology platform:

We provide Luminex technology for PDK-1 signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of laser technology, colored microspheres, high-speed digital and applied fluidics signal processing technology. The core is polypropylene microspheres encoded with magnetic microspheres or fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, more than 100 microspheres with different fluorescence spectra can be obtained. Perform ligand-receptor, enzyme-substrate, antigen-antibody binding reactions and nucleic acid hybridization reactions on microspheres with different fluorescent codes. Quantitative and qualitative analysis of microsphere encoding and reporter fluorescence were performed by laser detection.

The PDK-1 signaling pathway is essential to control the survival and proliferation of cells. It can enhance the therapeutic effect and overcome the resistance of currently used molecularly targeted drugs.

In addition to Luminex multiplex assay, enzyme-linked immunosorbent assay (ELISA), flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of PDK-1 signaling pathway detection:

• Good repeatability: The Luminex detection platform directly reads the fluorescence value, which is more stable and sensitive: 100 microspheres of each type are detected, and the median value is taken as the result, which is equivalent to 100 repeated detections for each sample.
• Low cost: Simultaneous detection of multiple indicators of a sample can save time, samples, reduce detection costs, and improve analysis efficiency.

Application of our service:

• Study the regulatory mechanism of PDK-1 signaling pathway in medicine
• Research on the influence of various pathogens on PDK-1 signaling pathway
• Study the effect of clinical drugs on PDK-1 signaling pathway

Creative Proteomics has developed a signal path target detection platform. We can not only provide customers with PDK-1 signal path detection services, but also other signal path detection services. If you want to detect other targets, please contact us in time, we will customize the service for you according to your needs. Looking forward to working with you.

References:

1. Mora A, Komander D, et al. PDK1, the master regulator of AGC kinase signal transduction. Semin Cell Dev Biol, 2004, 15:161-170.
2. Alessi D R, Deak M, et al. 3-Phosphoinositide-dependent protein kinase-1 (PDK 1): structural and functional homology with the Drosophila DSTPK61 kinase. Curr Biol, 1997, 7:776-789.