What is Olink Proteomics?
Proteins are increasingly emphasized in research as functional performers of life activities. Due to translational and post-translational regulation, the correlation between RNA expression and actual protein content is not high, and the correlation between low-abundance proteins and mRNAs is especially low. However, most low-abundance proteins, such as protein kinases and transcription factors, are favorite targets for research and clinical studies. Therefore, even with high-throughput methods such as RNA-seq, there is a need to characterize and quantify the proteins themselves directly.
Olink Proteomics' core patented technology, PEA-Neighborhood Extension, provides reliable technical support for precision proteomics, which can effectively tap into the mechanism of disease development, improve the detection rate of diseases, and discover more high-quality biomarkers.
Creative Proteomics has introduced Olink proteomics panel to provide protein biomarker analysis.
Olink Proteomics Panel Provided by Creative Proteomics
| Type | Protein Markers Included in Panel |
|---|---|
| Olink Inflammation Panel | ADA, ARTN, AXIN1, Beta-NGF, CASP-8, CCL3, CCL4, CCL19, CCL20, CCL23, CCL25, CCL28, CD40, CDCP1, CXCL1, CXCL5, CXCL6, CXCL9, CXCL10, CXCL11, CST5, DNER, CCL11, EIF4EBP1, FGF-21, FGF-23, FGF-5, FGF-19, Flt3L, LIF-R, CSF-1, MMP-1, MMP-10, MCP-1, MCP-2, MCP-3, MCP-4, CD244, NT-3, NRTN, OSM, OPG, PD-L1, EN-RAGE, SLAMF1, SIRT2, CX3CL1, GDNF, HGF, IFN-gamma, IL-1 alpha, IL-2, IL-2RB, IL-4, IL5, IL6, IL-7, IL-8, IL10, IL-10RA, IL-10RB, IL-12B, IL-13, IL-15RA, IL-17A, IL-17C, IL-18, IL-18R1, IL-20, IL-20RA, IL-22 RA1, IL-24, IL-33, TGFB1, LIF, STAMBP, SCF, ST1A1, CD6, CD5, CD8A, TSLP, TNFB, TRANCE, TRAIL, TGF-alpha, Ligand, TNF, TNFSF14, TNFRSF9, PLAU, VEGF-A |
| Olink Immune Response Panel | MILR1, AREG, ARNT, BIRC2, GLB1, BTN3A2, CD83, CNTNAP2, CDSN, HSD11B1, CXADR, CLEC4A, CLEC4C, CLEC4D, CLEC4G, CLEC6A, CLEC7A, CKAP4, DGKZ, DCBLD2, DFFA, DAPP1, DCTN1, TRIM21, EGLN1, CCL11, EIF4G1, EIF5A, FCRL3, KLRD1, PPP1R9B, NTF4, NFATC3, PTH1R, PSIP1, PRDX1, PRDX5, PIK3AP1, PLXNA4, GALNT3, DDX58, FAM3B, HEXIM1, PRKCQ, SPRY2, PADI2, FCRL6, FGF2, FXYD5, HCLS1, HNMT, KPNA1, DPP10, ITM2A, ITGA6, ITGA11, ITGB6, IFNLR1, IRF9, IRAK1, IRAK4, IL5, IL6, IL10, IL12RB1, ICA1, KRT19, LILRB4, LAG3, LY75, LAMP3, MASP1, NF2, MGMT, NCR1, SH2D1A, SH2B3, SIT1, SRPK2, STC1, CXCL12, CD28, PRDX3, TRAF2, TANK, JUN, BACH1, TREM1, TRIM5, TPSAB1, EDAR, ZBTB16 |
| Olink Immuno Oncology Panel | ADA, ADGRG1, ANGPT1, TIE2, ANGPT2, ARG1, CAIX, CASP-8, MCP-4, CCL17, CCL19, MCP-1, CCL20, CCL23, CCL3, CCL4, MCP-3, MCP-2, CD27, CD40-L, CD40, CD70, CD83, CXCL1, CXCL10, CXCL11, CXCL13, CXCL5, CXCL9, CRTAM, CSF-1, MMP12, MMP7, MIC-A/B, MUC-16, NCR1, CD244, KLRD1, NOS3, PGF, PDGF subunit B, PTN, EGF, PD-L1, PD-L2, PDCD1, DCN, FGF2, CX3CL1, Gal-1, Gal-9, GZMA, GZMB, GZMH, HO-1, HGF, ICOSLG, IFN-gamma, IL-1 alpha, IL10, IL12, IL12RB1, IL13, IL15, IL18, IL2, IL33, IL4, IL5, IL6, IL7, IL8, KIR3DL1, LAP TGF-beta-1, LAG3, LAMP3, CXCL12, CD4, CD5, CD8A, CD28, TRAIL, TNF, TWEAK, TNFSF14, FASLG, TNFRSF12A, TNFRSF21, TNFRSF4, TNFRSF9, VEGFA, VEGFR-2 |
| Olink Oncology II Panel | NT5E, ADAMTS15, TXLNA, AREG, ANXA1, CAIX, CPE, CEA, CEACAM1, CTSV, CD27, CD160, CD48, CD70, CRNN, CD207, CXCL13, CDKN1A, DLL1, ADAM8, ESM-1, EPHA2, FasL, FADD, FCRLB, FGF-BP1, FR-alpha, FR-gamma, FUR, S100A4, RET, ErbB2/HER2, ErbB3/HER3, ErbB4/HER4, RSPO3, SCAMP3, SEZ6L, SPARC, SCF, SYND1, TCL1A, TGFR-2, TFPI-2, LY9, TRAIL, TLR3, Gal-1, GPC1, GZMB, GZMH, HGF, ICOSLG, IGF1R, ITGAV, ITGB5, IFN-gamma-R1, IL6, hK8, hK11, KLK13, hK14, LYPD3, MIA, MSLN, MetAP 2, MIC-A/B, MK, MAD homolog 5, MUC-16, PVRL4, PPY, PODXL, EGF, CYR61, S100A11, TGF-alpha, GPNMB, TNFSF13, TNFRSF4, TNFRSF6B, TNFRSF19, ABL1, LYN, WFDC2, VEGF-A, VEGFR-2, VEGFR-3, CXL17, VIM, WIF-1, WISP-1, XPNPEP2 |
| Olink Oncology III Panel | PSMD9, GGA1, AKR1B1, ALPP, AIF1, ACTN4, AFP, AIMP1, CD22, RFNG, HEXA, CDHR2, CALCOCO1, CLIP2, CASP2, CDC27, GPA33, CBLN4, CGB3, CD300E, COL9A1, C1QA, CXCL14, LAP3, NT5C3A, DCTPP1, DRG2, ELOA, ERP44, LACTB2, CNPY2, NELL1, S100A16, PTP4A1, RP2, PCDH1, SFTPA1, RAB6A, FLT3, PTPRM, ARHGAP25, FUS, SCGN, SCG2, SCLY, 44448, GFER, TBL1X, FOXO3, USO1, GOPC, GAMT, HSPB6, HBQ1, HLA-E, JCHAIN, INPP1, ICAM4, ICAM5, IFNGR2, IL1B, IL17F, KLK4, LSP1, MANSC1, GALNT7, L1CAM, NCS1, NAMPT, ACAA1, PSPN, HMBS, CPVL, MLN, NPY, BRK1, SPINK4, VAT1, CCT5, TPMT, TXD15, TPT1, TNFAIP8, UBAC1, MYO9B, VPS37A, VPS53, FLT1, VMO1, VWA1, ATP6V1D, YTHDF3 |
| Olink Cardiovascular II Panel | DECR1, ADAMTS13, ADM, AGRP, IDUA, ANG-1, TIE2, ACE2, BMP-6, Protein BOC, CA5A, CEACAM8, CTSL1, CCL3, CCL17, CD40-L, CTRC, CXCL1, DCN, Dkk-1, FABP2, FGF-21, FGF-23, FS, Gal-9, GIF, GT, GH, GDF-2, PIgR, PD-L2, HB-EGF, IL16, PRELP, PRSS8 , AMBP, PAR-1, TGM2, SRC, PSGL-1, RAGE, REN, PRSS27, STK4, SERPINA12, CD84, HSP 27, HO-1, HAOX1, IL-1ra, IL1RL2, IL-4RA, IL6, IL-17D, IL-18, IL-27, KIM1, GLO1, LOX-1, LEP, LPL, FCGR2B, XCL1, MARCO, MMP-7, MMP-12, ITGB1BP2, BNP, NEMO, hOSCAR, PAPPA, PTX3, PGF, PDGF subunit B, PARP-1, SLAMF7, SORT1, SPON2, SCF, SOD2, CD4, THBD, THPO, THBS2, TF, TRAIL-R2, TNFRSF10A, TNFRSF11A, TNFRSF13B, MERTK, VEGFD, VSIG2 |
| Olink Cardiovascular III Panel | AP-N, AZU1, BLM hydrolase, CDH5, CPA1, CPB1, CASP-3, CTSD, CTSZ, CCL15, CCL16, CCL24, ALCAM, CHI3L1, CHIT1, COL1A1, CD93, CNTN1, CXCL16, CSTB, PI3, EPHB4, EGFR, Ep-CAM, SELE, FABP4, Gal-3, Gal-4, GRN, GDF-15, PLC, PAI, PECAM-1, PDGF subunit A, GP6, PCSK9, DLK-1, SELP, PSP-D, RETN, RARRES2, CD163, SCGB3A2, SPON1, ST2, TR-AP, IGFBP-1, IGFBP-2, IGFBP-7, ITGB2, ICAM-2, IL-1RT1, IL-1RT2, IL2-RA, IL-6RA, IL-17RA, IL-18BP, JAM-A, KLK6, LDL receptor, LTBR, MEPE, MMP-2, MMP-3, MMP-9, TIMP4, MCP-1, PRTN3, MPO, MB, Notch 3, NT-proBNP, OPN, OPG, PON3, PGLYRP1, TFPI, t-PA, TR, TFF3, TLT-2, TNFSF13B, TNF-R1, TNF-R2, FAS, TNFRSF10C, TNFRSF14, AXL, SHPS-1, U-PAR, uPA, vWF |
| Olink Cardiometabolic Panel | ANG, ANGPTL3, APOM, CNDP1, ST6GAL1，CDH1, CA1, CA3, CA4, CRTAC1, COMP, CCL5, CCL14, CCL18, CD59, F7, F11, COL18A1, C1QTNF1, C2, CFHR5, CR2, CST3, DPP4, EFEMP1, ENG, FETUB, FCN2, QPCT, GNLY, NRP1, DEFA1, LCN2, NID1, OSMR, PAM, PLTP, SERPINA5, GP1BA, PLA2G7, PLXNB2, PCOLCE, FAP, PTPRS, REG3A, SAA4, GAS6, MET, IGLC2, IGFBP3, IGFBP6, ITGAM, ICAM1, ICAM3, IL7R, LTBP2, LILRB1, LILRB2, LILRB5, REG1A, CES1, FCGR2A, FCGR3B, SELL, LYVE1, PRCP, MBL2, KIT, CD46, AOC3, TIMP1, MFAP5, MEGF9, NCAM1, CHL1, NOTCH1, SPARCL1, SOD1, TIMD4, TNC, TNXB, THBS4, SERPINA7, TCN2, TGFBR3, TGFBI, PRSS2, TIE1, UMOD, VCAM1, VASN, PROC |
| Olink Metabolism Panel | AHCY, ADGRE2, ADGRG2, APLP1, ANGPT2, ANGPTL1, ANGPTL7, ANXA4, ANXA11, GHRL, ARG1, DDC, CD79B, CDH2, CDHR5, CLSTN2, CA13, COMT, CTSO, CD2AP, CHRDL2, CLUL1, CCDC80, CRKL, CLEC5A, CLMP, DIABLO, QDPR, DPP7, DAB2, PILRB, FKBP4, PAG1, CTSH, FAM3C, PPP1R2, S100P, REG4, RTN4R, ALDH1A1, NQO2, SSC4D, SOST, SEMA3F, SERPINB6, SERPINB8, APEX1, ENTPD5, ENPP7, RNASE3, FCRL1, FBP1, GAL, ENO2, GLRX, GRAP2, HDGF, ROR1, IGFBPL1, ITGB7, KLK10, KYAT1, BAG6, LRIG1, LILRA5, LRP11, ACP6, MEP1B, METRNL, MCFD2, NADK, NECTIN2, NPDC1, NPTXR, NOMO1, NT-proBNP, SIGLEC7, CD164, CANT1, SUMF2, SNAP23, SDC4, CD1C, THOP1, TXNDC5, TYMP, TSHB, TFF2, TINAGL1, TYRO3, USP8, VCAN |
| Olink Cell Regulation Panel | AMIGO2, APBB1IP, AGR3, ARSB, PFKM, BCL2L11, GCNT1, BGN, SULT2A1, BCR, BOC, CAMKK1, CLSTN3, LYAR, TP53, CDNF, COL4A1, CRX, CFC1, MRC2, ATG4A, CRISP2, DKKL1, DCBLD2, DNAJB1, MAP2K6, DCTN2, CBL, ENTPD6, CPXM1, PROK1, PREB, FAM19A5, WNT9A, PCDH17, IQGAP2, RGS8, ARHGAP1, ARHGEF12, SEZ6L2, SEMA4C, PAK4, SIGLEC10, SIGLEC6, SLAMF8, SLITRK2, FGF21, FLI1, LGALS7, GSAP, GKN1, GFRA2, GCG, GH2, HS3ST3B1, HS6ST1, IGSF3, IL17RB, KLK12, KAZALD1, LRRN1, LYPD1, LRMP, METAP1D, MOG, DDAH1, NFKBIE, NCLN, NINJ1, NFATC1, OMG, OPTC, PRDX6, PODXL2, GALNT2, SLITRK6, SKAP1, STX6, STX16, TCL1B, JUN, TACC3, TDRKH, TNFRSF10A, TACSTD2, VEGFD, VAMP5, SORCS2, WASF1, WASF3, ZBTB16, ZBTB17 |
| Olink Neurology Panel | CD38, LRPAP1, NTRK2, Beta-NGF, BMP-4, BCAN, VWC2, CDH3, CDH6, CPA2, CPM, CTSS, CADM3, CD200R1, CLM-1, CLM-6, CNTN5, CLEC1B, CLEC10A, CRTAM, Dkk-4, CTSC, ADAM 22, ADAM 23, DRAXIN, EPHB6, EFNA4, DDR1, NEP, UNC5C, NBL1, NCAN, Nr-CAM, NRP2, N-CDase, NMNAT1, N2DL-2, NTRK3, CD200, PDGF-R-alpha, PLXNB1, PLXNB3, PVR, PRTG, RGMA, RGMB, EZR, FcRL2, gal-8, GFR-alpha-1, GDNFR-alpha-3, GDNF, GCP5, G-CSF, CSF2RA, GZMA, GDF-8, HAGH, IL-5R-alpha, IL-12, JAM-B, KYNU, LXN, LAYN, FLRT2, LAIR-2, LAT, SCARB2, MSR1, MDGA1, MATN3, MANF, MAPT, NAAA, ROBO2, RSPO1, SCARA5, SCARF2, sFRP-3, SKR3, Siglec-9, SIGLEC1, SMOC2, SMPD1, TN-R, SPOCK1, THY 1, TMPRSS5, TNFRSF12A, TNFRSF21, EDA2R, WFIKKN1 |
| Olink Neuro Exploratory Panel | PTS, ADGRB3, AARSD1, FUT8, AOC1, ANXA10, ASGR1, DEFB4A, KLB, FHIT, BST2, CDH15, CDH17, PPP3R1, CARHSP1, CLSTN1, CEACAM3, CTF1, CCL27, CD302, CD63, CETN2, COL4A3BP, CRIP2, CRADD, DSG3, DPEP1, DPEP2, ADAM15, DUSP3, NAA10, WWP2, UBE2F, NXPH1, NEFL, NPM1, FKBP5, FKBP7, PHOSPHO1, PMVK, PRTFDC1, PFDN2, PSG1, EREG, AKT1S1, PSME1, RNF31, HSP90B1, ECE1, EPHA10, EIF4B, FGFR2, IFI30, SNCG, GGT5, GSTP1, PAEP, PLA2G10, GBP2, HMOX2, FCAR, ISLR2, ING1, IMPA1, ILKAP, IFNL1, IL15, IL3RA, IL32, KIF1BP, KIR2DL3, KIRREL2, LTBP3, LEPR, MAD1L1, CD33, ABHD14B, NDRG1, RBKS, RPS6KB1, SFRP1, SRP14, SMOC1, TDGF1, GPNMB, TPPP3, TBCB, TNFRSF13C, PTPN1, VSTM1, ATP6V1F, IKZF2 |
| Olink Development Panel | BST1, NUDT5, ACAN, APP, ANGPTL4, ARSA, BCAM, B4GALT1, B4GAT1, GUSB, MSMB, CA2, CA6, CTSF, IGF2R, CCL21, CD97, CD99L2, CD109, CD177, CD209, CDON, CD300LG, COCH, COLEC12, CNTN4, CRHBP, CLEC11A, CLEC14A, CST6, MIF, MATN2, MYOC, NID2, OMD, PILRA, PPIB, PEBP1, PEAR1, PDGFRB, PARK7, P4HB, NOV, PTPRF, ROBO1, CRIM1, CRELD2, PLA2G4A, DSC2, DKK3, DAG1, CD69, ESAM, FCRL5, BLVRB, FSTL3, FUT3/5, CGA, XG, HAVCR2, CD74, PAMR1, INHBC, ITGA5, ITGB1, IL13RA1, SPINT1, SPINT2, MFGE8, LAMA4, MESDC2, LGMN, LAIR1, FCER2, CD58, SCARF1, SCGB3A1, SEMA7A, HTRA2, SPINK1, SPINK5, SIRPB1, STIP1, SNAP29, TMSB10, FUCA1, TPP1, RELT, PTPN6, WFIKKN2, VSIG4, CCN5 |
| Olink Organ Damage Panel | PRKAB1, ADGRG1, ALDH3A1, AGR2, AIFM1, BANK1, BID, BAMBI, CALCA, CALR, CA12, CA14, CSNK1D, CLSPN, ST3GAL1, CES2, CNTN2, CRH, CLEC1A, NPPC, DSG4, DPP6, TOP2B, ENTPD2, ENTPD6, EDIL3, TMPRSS15, EGFL7, ERBIN, PLIN1, INPPL1, PGF, PDGFC, PTN, PLXDC1, GALNT10, BTC, PDCD1, LRP1, PSMA1, AMN, ENAH, FOSB, MAX, PPM1B, PDP1, EPO, FABP9, FOXO1, TIGAR, HPGDS, PTK7, ITGB1BP1, PRKRA, KIM1, KIR3DL1, LTA4H, LAT2, LHB, MAEA, CAPG, MAGED1, METAP1, MVK, MAP4K5, SMAD1, SIRT5, NUB1, NCF2, NBN, NOS3, NUCB2, PVALB, PXN, FKBP1B, RASSF2, RASA1, PTPRJ, ATP6AP2, RCOR1, RARRES1, RRM2B, SERPINA9, PON2, STX8, STXBP3, TNNI3, FES, FGR, YES1, VASH1, WAS |
Advantages of Olink Proteomics Panel
- Low starting volume, high throughput: 48~3,072 biomarkers can be accurately detected in 1~6µl of body fluid at one time
- High sensitivity and specificity: unique PEA technology can detect proteins at fg/ml level and recognize homologous proteins with 90% similarity.
- Rigorous quality control system: internal and external controls are set up to strictly control the accuracy of the assay.
- Wide range of sample types: in addition to routine serum and plasma, the test can also be performed on cerebrospinal fluid, cell culture fluid, tissue lysate, and other sample types.
Applications of Olink Proteomics Panel
Clinical disease research: disease mechanism, disease classification, disease diagnosis and prognostic molecular markers, etc.
Drug research: drug targets, drug mechanism of action, etc.
Industrial customer research: assisting new drug development, new diagnostic product development, etc.
Sample Recommendations for Olink Proteomics Service
| Sample Type | Sample Volume (96-well PCR plate) | Sample Volume (EP tube) | Preservation and Transportation |
|---|---|---|---|
| Serum/Plasma | ≥50 ul | ≥100 ul | Liquid nitrogen quick freeze. -80°C Storage. Dry ice transportation. |
| Cell Supernatant | ≥50 ul | ≥100 ul | |
| Tissue Lysate | ≥50 ul | ≥100 ul | |
| Body Fluid | ≥50 ul | ≥100 ul |
Protein Biomarker Discovery Workflow
