Olink Proteomics Service

What is Olink Proteomics?

Proteins are increasingly emphasized in research as functional performers of life activities. Due to translational and post-translational regulation, the correlation between RNA expression and actual protein content is not high, and the correlation between low-abundance proteins and mRNAs is especially low. However, most low-abundance proteins, such as protein kinases and transcription factors, are favorite targets for research and clinical studies. Therefore, even with high-throughput methods such as RNA-seq, there is a need to characterize and quantify the proteins themselves directly.

Olink Proteomics' core patented technology, PEA-Neighborhood Extension, provides reliable technical support for precision proteomics, which can effectively tap into the mechanism of disease development, improve the detection rate of diseases, and discover more high-quality biomarkers.

Creative Proteomics has introduced Olink proteomics panel to provide protein biomarker analysis.

Olink Proteomics Panel Provided by Creative Proteomics

Type	Protein Markers Included in Panel
Olink Inflammation Panel	ADA, ARTN, AXIN1, Beta-NGF, CASP-8, CCL3, CCL4, CCL19, CCL20, CCL23, CCL25, CCL28, CD40, CDCP1, CXCL1, CXCL5, CXCL6, CXCL9, CXCL10, CXCL11, CST5, DNER, CCL11, EIF4EBP1, FGF-21, FGF-23, FGF-5, FGF-19, Flt3L, LIF-R, CSF-1, MMP-1, MMP-10, MCP-1, MCP-2, MCP-3, MCP-4, CD244, NT-3, NRTN, OSM, OPG, PD-L1, EN-RAGE, SLAMF1, SIRT2, CX3CL1, GDNF, HGF, IFN-gamma, IL-1 alpha, IL-2, IL-2RB, IL-4, IL5, IL6, IL-7, IL-8, IL10, IL-10RA, IL-10RB, IL-12B, IL-13, IL-15RA, IL-17A, IL-17C, IL-18, IL-18R1, IL-20, IL-20RA, IL-22 RA1, IL-24, IL-33, TGFB1, LIF, STAMBP, SCF, ST1A1, CD6, CD5, CD8A, TSLP, TNFB, TRANCE, TRAIL, TGF-alpha, Ligand, TNF, TNFSF14, TNFRSF9, PLAU, VEGF-A
Olink Immune Response Panel	MILR1, AREG, ARNT, BIRC2, GLB1, BTN3A2, CD83, CNTNAP2, CDSN, HSD11B1, CXADR, CLEC4A, CLEC4C, CLEC4D, CLEC4G, CLEC6A, CLEC7A, CKAP4, DGKZ, DCBLD2, DFFA, DAPP1, DCTN1, TRIM21, EGLN1, CCL11, EIF4G1, EIF5A, FCRL3, KLRD1, PPP1R9B, NTF4, NFATC3, PTH1R, PSIP1, PRDX1, PRDX5, PIK3AP1, PLXNA4, GALNT3, DDX58, FAM3B, HEXIM1, PRKCQ, SPRY2, PADI2, FCRL6, FGF2, FXYD5, HCLS1, HNMT, KPNA1, DPP10, ITM2A, ITGA6, ITGA11, ITGB6, IFNLR1, IRF9, IRAK1, IRAK4, IL5, IL6, IL10, IL12RB1, ICA1, KRT19, LILRB4, LAG3, LY75, LAMP3, MASP1, NF2, MGMT, NCR1, SH2D1A, SH2B3, SIT1, SRPK2, STC1, CXCL12, CD28, PRDX3, TRAF2, TANK, JUN, BACH1, TREM1, TRIM5, TPSAB1, EDAR, ZBTB16
Olink Immuno Oncology Panel	ADA, ADGRG1, ANGPT1, TIE2, ANGPT2, ARG1, CAIX, CASP-8, MCP-4, CCL17, CCL19, MCP-1, CCL20, CCL23, CCL3, CCL4, MCP-3, MCP-2, CD27, CD40-L, CD40, CD70, CD83, CXCL1, CXCL10, CXCL11, CXCL13, CXCL5, CXCL9, CRTAM, CSF-1, MMP12, MMP7, MIC-A/B, MUC-16, NCR1, CD244, KLRD1, NOS3, PGF, PDGF subunit B, PTN, EGF, PD-L1, PD-L2, PDCD1, DCN, FGF2, CX3CL1, Gal-1, Gal-9, GZMA, GZMB, GZMH, HO-1, HGF, ICOSLG, IFN-gamma, IL-1 alpha, IL10, IL12, IL12RB1, IL13, IL15, IL18, IL2, IL33, IL4, IL5, IL6, IL7, IL8, KIR3DL1, LAP TGF-beta-1, LAG3, LAMP3, CXCL12, CD4, CD5, CD8A, CD28, TRAIL, TNF, TWEAK, TNFSF14, FASLG, TNFRSF12A, TNFRSF21, TNFRSF4, TNFRSF9, VEGFA, VEGFR-2
Olink Oncology II Panel	NT5E, ADAMTS15, TXLNA, AREG, ANXA1, CAIX, CPE, CEA, CEACAM1, CTSV, CD27, CD160, CD48, CD70, CRNN, CD207, CXCL13, CDKN1A, DLL1, ADAM8, ESM-1, EPHA2, FasL, FADD, FCRLB, FGF-BP1, FR-alpha, FR-gamma, FUR, S100A4, RET, ErbB2/HER2, ErbB3/HER3, ErbB4/HER4, RSPO3, SCAMP3, SEZ6L, SPARC, SCF, SYND1, TCL1A, TGFR-2, TFPI-2, LY9, TRAIL, TLR3, Gal-1, GPC1, GZMB, GZMH, HGF, ICOSLG, IGF1R, ITGAV, ITGB5, IFN-gamma-R1, IL6, hK8, hK11, KLK13, hK14, LYPD3, MIA, MSLN, MetAP 2, MIC-A/B, MK, MAD homolog 5, MUC-16, PVRL4, PPY, PODXL, EGF, CYR61, S100A11, TGF-alpha, GPNMB, TNFSF13, TNFRSF4, TNFRSF6B, TNFRSF19, ABL1, LYN, WFDC2, VEGF-A, VEGFR-2, VEGFR-3, CXL17, VIM, WIF-1, WISP-1, XPNPEP2
Olink Oncology III Panel	PSMD9, GGA1, AKR1B1, ALPP, AIF1, ACTN4, AFP, AIMP1, CD22, RFNG, HEXA, CDHR2, CALCOCO1, CLIP2, CASP2, CDC27, GPA33, CBLN4, CGB3, CD300E, COL9A1, C1QA, CXCL14, LAP3, NT5C3A, DCTPP1, DRG2, ELOA, ERP44, LACTB2, CNPY2, NELL1, S100A16, PTP4A1, RP2, PCDH1, SFTPA1, RAB6A, FLT3, PTPRM, ARHGAP25, FUS, SCGN, SCG2, SCLY, 44448, GFER, TBL1X, FOXO3, USO1, GOPC, GAMT, HSPB6, HBQ1, HLA-E, JCHAIN, INPP1, ICAM4, ICAM5, IFNGR2, IL1B, IL17F, KLK4, LSP1, MANSC1, GALNT7, L1CAM, NCS1, NAMPT, ACAA1, PSPN, HMBS, CPVL, MLN, NPY, BRK1, SPINK4, VAT1, CCT5, TPMT, TXD15, TPT1, TNFAIP8, UBAC1, MYO9B, VPS37A, VPS53, FLT1, VMO1, VWA1, ATP6V1D, YTHDF3
Olink Cardiovascular II Panel	DECR1, ADAMTS13, ADM, AGRP, IDUA, ANG-1, TIE2, ACE2, BMP-6, Protein BOC, CA5A, CEACAM8, CTSL1, CCL3, CCL17, CD40-L, CTRC, CXCL1, DCN, Dkk-1, FABP2, FGF-21, FGF-23, FS, Gal-9, GIF, GT, GH, GDF-2, PIgR, PD-L2, HB-EGF, IL16, PRELP, PRSS8 , AMBP, PAR-1, TGM2, SRC, PSGL-1, RAGE, REN, PRSS27, STK4, SERPINA12, CD84, HSP 27, HO-1, HAOX1, IL-1ra, IL1RL2, IL-4RA, IL6, IL-17D, IL-18, IL-27, KIM1, GLO1, LOX-1, LEP, LPL, FCGR2B, XCL1, MARCO, MMP-7, MMP-12, ITGB1BP2, BNP, NEMO, hOSCAR, PAPPA, PTX3, PGF, PDGF subunit B, PARP-1, SLAMF7, SORT1, SPON2, SCF, SOD2, CD4, THBD, THPO, THBS2, TF, TRAIL-R2, TNFRSF10A, TNFRSF11A, TNFRSF13B, MERTK, VEGFD, VSIG2
Olink Cardiovascular III Panel	AP-N, AZU1, BLM hydrolase, CDH5, CPA1, CPB1, CASP-3, CTSD, CTSZ, CCL15, CCL16, CCL24, ALCAM, CHI3L1, CHIT1, COL1A1, CD93, CNTN1, CXCL16, CSTB, PI3, EPHB4, EGFR, Ep-CAM, SELE, FABP4, Gal-3, Gal-4, GRN, GDF-15, PLC, PAI, PECAM-1, PDGF subunit A, GP6, PCSK9, DLK-1, SELP, PSP-D, RETN, RARRES2, CD163, SCGB3A2, SPON1, ST2, TR-AP, IGFBP-1, IGFBP-2, IGFBP-7, ITGB2, ICAM-2, IL-1RT1, IL-1RT2, IL2-RA, IL-6RA, IL-17RA, IL-18BP, JAM-A, KLK6, LDL receptor, LTBR, MEPE, MMP-2, MMP-3, MMP-9, TIMP4, MCP-1, PRTN3, MPO, MB, Notch 3, NT-proBNP, OPN, OPG, PON3, PGLYRP1, TFPI, t-PA, TR, TFF3, TLT-2, TNFSF13B, TNF-R1, TNF-R2, FAS, TNFRSF10C, TNFRSF14, AXL, SHPS-1, U-PAR, uPA, vWF
Olink Cardiometabolic Panel	ANG, ANGPTL3, APOM, CNDP1, ST6GAL1，CDH1, CA1, CA3, CA4, CRTAC1, COMP, CCL5, CCL14, CCL18, CD59, F7, F11, COL18A1, C1QTNF1, C2, CFHR5, CR2, CST3, DPP4, EFEMP1, ENG, FETUB, FCN2, QPCT, GNLY, NRP1, DEFA1, LCN2, NID1, OSMR, PAM, PLTP, SERPINA5, GP1BA, PLA2G7, PLXNB2, PCOLCE, FAP, PTPRS, REG3A, SAA4, GAS6, MET, IGLC2, IGFBP3, IGFBP6, ITGAM, ICAM1, ICAM3, IL7R, LTBP2, LILRB1, LILRB2, LILRB5, REG1A, CES1, FCGR2A, FCGR3B, SELL, LYVE1, PRCP, MBL2, KIT, CD46, AOC3, TIMP1, MFAP5, MEGF9, NCAM1, CHL1, NOTCH1, SPARCL1, SOD1, TIMD4, TNC, TNXB, THBS4, SERPINA7, TCN2, TGFBR3, TGFBI, PRSS2, TIE1, UMOD, VCAM1, VASN, PROC
Olink Metabolism Panel	AHCY, ADGRE2, ADGRG2, APLP1, ANGPT2, ANGPTL1, ANGPTL7, ANXA4, ANXA11, GHRL, ARG1, DDC, CD79B, CDH2, CDHR5, CLSTN2, CA13, COMT, CTSO, CD2AP, CHRDL2, CLUL1, CCDC80, CRKL, CLEC5A, CLMP, DIABLO, QDPR, DPP7, DAB2, PILRB, FKBP4, PAG1, CTSH, FAM3C, PPP1R2, S100P, REG4, RTN4R, ALDH1A1, NQO2, SSC4D, SOST, SEMA3F, SERPINB6, SERPINB8, APEX1, ENTPD5, ENPP7, RNASE3, FCRL1, FBP1, GAL, ENO2, GLRX, GRAP2, HDGF, ROR1, IGFBPL1, ITGB7, KLK10, KYAT1, BAG6, LRIG1, LILRA5, LRP11, ACP6, MEP1B, METRNL, MCFD2, NADK, NECTIN2, NPDC1, NPTXR, NOMO1, NT-proBNP, SIGLEC7, CD164, CANT1, SUMF2, SNAP23, SDC4, CD1C, THOP1, TXNDC5, TYMP, TSHB, TFF2, TINAGL1, TYRO3, USP8, VCAN
Olink Cell Regulation Panel	AMIGO2, APBB1IP, AGR3, ARSB, PFKM, BCL2L11, GCNT1, BGN, SULT2A1, BCR, BOC, CAMKK1, CLSTN3, LYAR, TP53, CDNF, COL4A1, CRX, CFC1, MRC2, ATG4A, CRISP2, DKKL1, DCBLD2, DNAJB1, MAP2K6, DCTN2, CBL, ENTPD6, CPXM1, PROK1, PREB, FAM19A5, WNT9A, PCDH17, IQGAP2, RGS8, ARHGAP1, ARHGEF12, SEZ6L2, SEMA4C, PAK4, SIGLEC10, SIGLEC6, SLAMF8, SLITRK2, FGF21, FLI1, LGALS7, GSAP, GKN1, GFRA2, GCG, GH2, HS3ST3B1, HS6ST1, IGSF3, IL17RB, KLK12, KAZALD1, LRRN1, LYPD1, LRMP, METAP1D, MOG, DDAH1, NFKBIE, NCLN, NINJ1, NFATC1, OMG, OPTC, PRDX6, PODXL2, GALNT2, SLITRK6, SKAP1, STX6, STX16, TCL1B, JUN, TACC3, TDRKH, TNFRSF10A, TACSTD2, VEGFD, VAMP5, SORCS2, WASF1, WASF3, ZBTB16, ZBTB17
Olink Neurology Panel	CD38, LRPAP1, NTRK2, Beta-NGF, BMP-4, BCAN, VWC2, CDH3, CDH6, CPA2, CPM, CTSS, CADM3, CD200R1, CLM-1, CLM-6, CNTN5, CLEC1B, CLEC10A, CRTAM, Dkk-4, CTSC, ADAM 22, ADAM 23, DRAXIN, EPHB6, EFNA4, DDR1, NEP, UNC5C, NBL1, NCAN, Nr-CAM, NRP2, N-CDase, NMNAT1, N2DL-2, NTRK3, CD200, PDGF-R-alpha, PLXNB1, PLXNB3, PVR, PRTG, RGMA, RGMB, EZR, FcRL2, gal-8, GFR-alpha-1, GDNFR-alpha-3, GDNF, GCP5, G-CSF, CSF2RA, GZMA, GDF-8, HAGH, IL-5R-alpha, IL-12, JAM-B, KYNU, LXN, LAYN, FLRT2, LAIR-2, LAT, SCARB2, MSR1, MDGA1, MATN3, MANF, MAPT, NAAA, ROBO2, RSPO1, SCARA5, SCARF2, sFRP-3, SKR3, Siglec-9, SIGLEC1, SMOC2, SMPD1, TN-R, SPOCK1, THY 1, TMPRSS5, TNFRSF12A, TNFRSF21, EDA2R, WFIKKN1
Olink Neuro Exploratory Panel	PTS, ADGRB3, AARSD1, FUT8, AOC1, ANXA10, ASGR1, DEFB4A, KLB, FHIT, BST2, CDH15, CDH17, PPP3R1, CARHSP1, CLSTN1, CEACAM3, CTF1, CCL27, CD302, CD63, CETN2, COL4A3BP, CRIP2, CRADD, DSG3, DPEP1, DPEP2, ADAM15, DUSP3, NAA10, WWP2, UBE2F, NXPH1, NEFL, NPM1, FKBP5, FKBP7, PHOSPHO1, PMVK, PRTFDC1, PFDN2, PSG1, EREG, AKT1S1, PSME1, RNF31, HSP90B1, ECE1, EPHA10, EIF4B, FGFR2, IFI30, SNCG, GGT5, GSTP1, PAEP, PLA2G10, GBP2, HMOX2, FCAR, ISLR2, ING1, IMPA1, ILKAP, IFNL1, IL15, IL3RA, IL32, KIF1BP, KIR2DL3, KIRREL2, LTBP3, LEPR, MAD1L1, CD33, ABHD14B, NDRG1, RBKS, RPS6KB1, SFRP1, SRP14, SMOC1, TDGF1, GPNMB, TPPP3, TBCB, TNFRSF13C, PTPN1, VSTM1, ATP6V1F, IKZF2
Olink Development Panel	BST1, NUDT5, ACAN, APP, ANGPTL4, ARSA, BCAM, B4GALT1, B4GAT1, GUSB, MSMB, CA2, CA6, CTSF, IGF2R, CCL21, CD97, CD99L2, CD109, CD177, CD209, CDON, CD300LG, COCH, COLEC12, CNTN4, CRHBP, CLEC11A, CLEC14A, CST6, MIF, MATN2, MYOC, NID2, OMD, PILRA, PPIB, PEBP1, PEAR1, PDGFRB, PARK7, P4HB, NOV, PTPRF, ROBO1, CRIM1, CRELD2, PLA2G4A, DSC2, DKK3, DAG1, CD69, ESAM, FCRL5, BLVRB, FSTL3, FUT3/5, CGA, XG, HAVCR2, CD74, PAMR1, INHBC, ITGA5, ITGB1, IL13RA1, SPINT1, SPINT2, MFGE8, LAMA4, MESDC2, LGMN, LAIR1, FCER2, CD58, SCARF1, SCGB3A1, SEMA7A, HTRA2, SPINK1, SPINK5, SIRPB1, STIP1, SNAP29, TMSB10, FUCA1, TPP1, RELT, PTPN6, WFIKKN2, VSIG4, CCN5
Olink Organ Damage Panel	PRKAB1, ADGRG1, ALDH3A1, AGR2, AIFM1, BANK1, BID, BAMBI, CALCA, CALR, CA12, CA14, CSNK1D, CLSPN, ST3GAL1, CES2, CNTN2, CRH, CLEC1A, NPPC, DSG4, DPP6, TOP2B, ENTPD2, ENTPD6, EDIL3, TMPRSS15, EGFL7, ERBIN, PLIN1, INPPL1, PGF, PDGFC, PTN, PLXDC1, GALNT10, BTC, PDCD1, LRP1, PSMA1, AMN, ENAH, FOSB, MAX, PPM1B, PDP1, EPO, FABP9, FOXO1, TIGAR, HPGDS, PTK7, ITGB1BP1, PRKRA, KIM1, KIR3DL1, LTA4H, LAT2, LHB, MAEA, CAPG, MAGED1, METAP1, MVK, MAP4K5, SMAD1, SIRT5, NUB1, NCF2, NBN, NOS3, NUCB2, PVALB, PXN, FKBP1B, RASSF2, RASA1, PTPRJ, ATP6AP2, RCOR1, RARRES1, RRM2B, SERPINA9, PON2, STX8, STXBP3, TNNI3, FES, FGR, YES1, VASH1, WAS

Advantages of Olink Proteomics Panel

Low starting volume, high throughput: 48~3,072 biomarkers can be accurately detected in 1~6µl of body fluid at one time
High sensitivity and specificity: unique PEA technology can detect proteins at fg/ml level and recognize homologous proteins with 90% similarity.
Rigorous quality control system: internal and external controls are set up to strictly control the accuracy of the assay.
Wide range of sample types: in addition to routine serum and plasma, the test can also be performed on cerebrospinal fluid, cell culture fluid, tissue lysate, and other sample types.

Applications of Olink Proteomics Panel

Clinical disease research: disease mechanism, disease classification, disease diagnosis and prognostic molecular markers, etc.

Drug research: drug targets, drug mechanism of action, etc.

Industrial customer research: assisting new drug development, new diagnostic product development, etc.

Sample Recommendations for Olink Proteomics Service

Sample Type	Sample Volume (96-well PCR plate)	Sample Volume (EP tube)	Preservation and Transportation
Serum/Plasma	≥50 ul	≥100 ul	Liquid nitrogen quick freeze. -80°C Storage. Dry ice transportation.
Cell Supernatant	≥50 ul	≥100 ul
Tissue Lysate	≥50 ul	≥100 ul
Body Fluid	≥50 ul	≥100 ul