Introduction
Creative Proteomics can simultaneously quantitatively detect hypertension-related cytokines and protein through a variety of technologies, and provide higher sensitivity and a wider detection range.
Inflammatory cells are the general term for cells that play an important role in the process of inflammation, including lymphocytes, monocytes/macrophages, mast cells, fibroblasts, etc. Hypertension is an obvious chronic inflammatory process characterized by activation of the lymphocyte system and the monocyte system. Inflammatory factors play a very important role in the occurrence, development and outcome of hypertension.
Cytokines involved in hypertension
C-reactive protein is an inflammatory marker of essential hypertension and can be used to predict the occurrence and development of hypertension.
Hypersensitive C-reactive protein (hs-CRP) belongs to the category of acute phase reactive protein. Accurate detection of low-concentration C-reactive protein can be used as a sensitive indicator to distinguish low-level inflammation.
TNF-α is mainly regulated in physiological functions, and has the functions of promoting immune regulation and anti-infection. TNF-α can participate in the local or systemic inflammatory reaction process of patients through neuro-endocrine mechanism, vascular injury and remodeling, cell proliferation and other forms. It is closely related to the occurrence and development of hypertension.
Some cytokines, such as IL-1, IL-6, IL-8, IL-10, IL-17 and IL-18, play a certain role in the occurrence and development of hypertension and the further damage to target organs. IL-1 stimulates the secretion of endothelin (ET-1) by vascular endothelial cells. ET-1 can constrict blood vessels, resulting in damage to blood vessel walls and aggravation of autoimmune response, thus making the body continue to maintain hypertension. IL-6 stimulates cellular immunity and humoral immunity to produce an inflammatory response, damages vascular endothelial cells, increases blood viscosity, and causes peripheral resistance blood vessels to contract. IL-6 can also effectively promote the proliferation of vascular smooth muscle cells and fibroblasts, and produce a large amount of platelet-derived growth factor (PDGF). The increase in IL-6 level can increase the calcium ion concentration in smooth muscle cells, thereby enhancing the contractile ability of blood vessels. IL-10 can regulate the immunosuppressive function of cells. IL-17 is one of the independent influencing factors of prehypertension. IL-18 is also involved in the occurrence and development of hypertension.
Detectable cytokines include but are not limited to:
| CRP | IFN-a | IFN-γ | IL-1α | IL-1β |
| IL-6 | IL-8 | IL-10 | IL-18 | TNF-α |
| TNF-β | MCP-1 | MIP-1α | MIP-1β | NGF |
Technology platform
We mainly provide the Luminex cytokine detection platform. Luminex uses fluorescently encoded microspheres with specific antibodies to different target molecules. The different microspheres can be combined freely to a certain extent so that up to 100 analytes can be tested multiple times simultaneously in a single experiment.
The Luminex cytokine assay platform has the following advantages:
- Multiple detection: simultaneous detection of 100 biological targets
- Short experiment time: 1-3 weeks
- High sensitivity: the lower limit of accurate quantification is as low as 0.1 pg/mL
- Save samples: only need a sample volume as low as 25 μL
- Time saving: the experiment process only takes 4 hours
For your different needs, we can also provide the following detection methods:
- Flow cytometry (FACS analysis): Identify and measure cell biomarkers in complex subpopulations. It can be used for intracellular cytokine detection without two hours.
- Enzyme-linked immunosorbent assay (ELISA): Use the primary antibody for capture, and conjugate the secondary antibody with an enzyme or radioisotope for detection. Our multiplexing system can detect the expression of multiple cytokines at once.
Sample preparation
- Suitable for serum, plasma, cell culture supernatant and all kinds of body fluids.
- Cells (whole blood cells, PBMC, mouse immune cells)
- The body fluid samples are stored in a refrigerator at -80 degrees Celsius and transported on dry ice.
Creative Proteomics can provide you with a one-stop solution. If you want to test other cytokines or other information you want to consult, please contact us. We are looking forward to cooperating with you.
Reference:
- Dai X, Hua L, Chen Y, et al. Mechanisms in hypertension and target organ damage: Is the role of the thymus key?. International journal of molecular medicine, 2018, 42(1): 3-12.