Introduction

Porcine Cytokine AssayPigs share close homology with humans, including similar heart size and flow rates, skin, liver enzymes, and bone healing. Therefore, the pig model has the ability to replace higher animals, such as canines and non-human primates for research in many areas of study, such as cardiovascular, toxicology, pharmacology, aging, and skeletal studies. In addition, "mini-pigs" have been bred to retain a heart size and function similar to that of humans, which can age without growing too large for long-term preclinical studies, thus allowing multi-generational studies to be conducted in a relatively short period of time, greatly increasing the use of porcine models in scientific research.

In order to determine the specific cytokines involved in any inflammation or immune response, cytokines may need to be screened, which usually requires a certain degree of automation and/or high throughput. Beads can make the process of automation and high-throughput screening easier. Creative Proteomics offers porcine cytokine assays based on Luminex assay technology, enabling the simultaneous determination of multiple proteins. The assay can be applied to extracellular protein analysis of virtually any type of biological sample. We will also provide additional technologies to assist in validation if you require.

Our detectable targets:

We offer a wide selection of target analytes, including but not limited to:

GM-CSFIFNγIL-1αIL-1βIL-2IL-4
IL-6IL-8IL-10IL-12IL-18TNFα

We also offer a wide range of established Porcine Luminex Cytokine Panels for you to choose from.

Technology platform

We implement cytokine detection services based on Luminex xMAP technology. xMAP is a microbead-based immunoassay. It utilizes fluorescent color-coded magnetic microspheres that are coated with a specific capture antibody against the target protein. To detect the protein captured on the beads, biotinylated antibodies (also specific for the protein) are mixed with the beads and a streptavidin-PE coupling. The subsequent biotin-streptavidin-PE coupling reaction allows identification of each microsphere by its unique color code (protein specificity) and quantification by fluorescence intensity (streptavidin-PE).

The Luminex cytokine assay platform has the following advantages:

For your different needs, we can also provide the following detection methods:

Sample preparation

Acceptable samples include pig serum, plasma, tissue/cell lysate and culture supernatant. Please contact technical staff for details.

Application

Creative Proteomics can provide you with a one-stop solution. If you want to detect other targets or ask for other information, please contact us. We are looking forward to cooperating with you.

* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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