Introduction to necroptosis signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of necroptosis signaling pathway.

Necroptosis Signaling Pathway Detection Service

Necroptosis, a recently discovered form of programmed cell death, is a cell death mechanism that has necrosis-like morphological characteristics. Necroptosis activation relies on the receptor-interacting protein (RIP) homology interaction motif (RHIM). A variety of RHIM-containing proteins transduce necroptotic signals from the cell trigger to the cell death mediators RIP3 and mixed lineage kinase domain-like protein (MLKL). RIP1 plays a particularly important and complex role in necroptotic cell death regulation ranging from cell death activation to inhibition, and these functions are often cell type and context dependent. Increasing evidence suggests that necroptosis plays an important role in the pathogenesis of neurodegenerative diseases. Moreover, small molecules such as necrostatin-1 are thought inhibit necroptotic signaling pathway. Understanding the precise mechanisms underlying necroptosis and its interactions with other cell death pathways in neurodegenerative diseases could provide significant therapeutic insights.

Necroptosis can be widely stimulated by tumor necrosis factor (TNF), other members of the TNF death ligand family (Fas and TNF-related apoptosis-inducing ligand (TRAIL)), interferons, Toll-like receptors (TLRs) signaling and viral infection via the DNA sensor DNAdependent activator of interferon regulatory factor (DAI). Various upstream signaling pathways share the common terminal mechanism executed by mixed lineage kinase domain-like proteins.

Blocking necroptotic pathways with synthetic inhibitors or genetic manipulation mitigates neurodegenerative disease in vitro and in vivo, which suggests a promising therapeutic strategy for neurodegenerative disease.

Our detectable targets:

ROCKGSK-3NIKBidAsk-1PKC
Cdc2ATG4JNKMYD88Rac1TLR4
ATG16WIPi2LC3IPROLC3ULKFIP200
VPS15VPS34UYRAGNF-κBRIG-1TRAF3
GASIRF9AMBRA1p38RIP1TRAF5
Histone-H3IRF5MEK3p38MAPKSH2TRAF6
IPS-1ISGF3MSK2PI3KTBK1Vav
IRAK1ISREmTORPKRTLR3 

Technology platform:

We provide Luminex technology for necroptosis signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform developed on the basis of colored microspheres, laser technology, applied fluidics and high-speed digital signal processing technology. The core is to encode polypropylene microspheres or magnetic microspheres with fluorescent dyes. By adjusting the different ratios of the two fluorescent dyes, up to 100 microspheres with different fluorescence spectra can be obtained. Antigen-antibody, enzyme-substrate, ligand-receptor binding reactions and nucleic acid hybridization reactions are performed on microspheres with different fluorescence encoding. Qualitative and quantitative analysis by laser detection of microsphere coding and reporter fluorescence separately.

Necroptosis is involved in many pathological processes, such as ischemia-reperfusion injury in the heart,11 brain,4 and in injury-induced inflammatory diseases. Necroptosis reportedly plays a critical role in the pathogenesis of several neurodegenerative diseases.

In addition to Luminex Multiplex Assay, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry (FACS analysis) technology can also be provided to meet other customer needs.

Advantages of necroptosis signaling pathway detection:

Necroptosis Signaling Pathway Detection Service

Application of our service:

Creative Proteomics has developed a signal pathway target detection platform. We are not limited to providing necroptosis signal path detection services, but can also provide other signal path detection services. If you want to detect other targets, please contact us and we will customize the service for you. Look forward to working with you.

References:

  1. Lalaoui N, Lindqvist LM, et al. The molecular relationships between apoptosis, autophagy and necroptosis. Semin Cell Dev Biol, 2015, 39: 63-69.
  2. Koshinuma S, Miyamae M, et al. Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia-reperfusion injury. J Anesth, 2014, 28: 235-241.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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