Adiponectin signaling pathway

Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of adiponectin signaling pathway.

Adiponectin Signaling Pathway Detection Service(Deepa S S, et al., 2019)Lipocalin, also known as Acrp30, is an adipocytokine that positively regulates glucose and lipid metabolism by increasing insulin sensitivity, stimulating fatty acid oxidation and glucose uptake, and inhibiting hepatic gluconeogenesis. Lipocalin deficiency is commonly associated with obesity and is highly correlated with insulin resistance, mitochondrial dysfunction and dyslipidemia. 2003 saw the identification of two lipocalin receptors, Adipo R1 and Adipo R2.3, with Adipo R1 being the primary lipocalin receptor expressed in skeletal muscle, which is the primary glucose-utilizing tissue in the body.

Lipocalin signaling promotes activation of PGC1 alpha and mitochondrial biogenesis. Lipocalin binding to Adipo R1 leads to calcium inward flow in skeletal muscle, which activates CaMKK β. Activated CaMKK β induces the expression and activation of PGC1 α through a cascade reaction that involves the activation of AMPK and increased production of SIRT1 deacetylase. AMPK phosphorylation and SIRT1 deacetylation of PGC1 α increase its transcriptional activity, leading to increased mitochondrial biogenesis.

Lipocalin, an adipokine secreted by white adipose tissue, plays an important role in regulating glucose and lipid metabolism in insulin-sensitive tissues and in controlling energy homeostasis. APPL1 is the first identified protein that interacts directly with the lipocalin receptor.

Our detectable targets:

RTKPI3KTYK2STAT1STAT2STAT3
PSGL1IRS2MSK1TLR3Tak1TRIF
TNFRISGF3MSK2PI3KTBK1Vav
STAT5IRAK4JNKMYD88Rac1TLR4
CD3IRF5MEK3p38MAPKSH2TRAF6
CREBIRF3MAPKNAP1RelTLR9
p38FasISREmTORPKRTRAF5
MHC-IIIRS1MEK6p50SLP76TRAM
TCRicam1Mda-5NFκBRIG-1TRAF3
GASIRF9MEKK1RIP1  

Technology platform:

We provide Luminex technology for adiponectin signaling pathway analysis.

Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.

Advantages of adiponectin signaling pathway detection:

Advantages of adiponectin signaling pathway detection

Application of our service:

Creative Proteomics has developed a signaling pathway target detection platform. We can provide detection services not only for adiponectin signaling pathways, but also for other signaling pathways. If you want to detect other targets, please contact us in advance and we will customize the service for you according to your requirements. Looking forward to working with you.

References:

  1. Deepa S S, Dong L Q. APPL1: role in adiponectin signaling and beyond. American Journal of Physiology Endocrinology and Metabolism, 2019, 296(1).
  2. Yamaguchi N, Argueta J, et al. Adiponectin inhibits Toll-like receptor family-induced signaling. FEBS Letters, 2020, 579(30): 6821-6826.
  3. Changhua, Wang, Xuming, et al. Adiponectin Sensitizes Insulin Signaling by Reducing p70 S6 Kinase-mediated Serine Phosphorylation of IRS-1. Journal of Biological Chemistry, 2017, 282(11): 7991-7996.
* For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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