Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of adiponectin signaling pathway.
(Deepa S S, et al., 2019)Lipocalin, also known as Acrp30, is an adipocytokine that positively regulates glucose and lipid metabolism by increasing insulin sensitivity, stimulating fatty acid oxidation and glucose uptake, and inhibiting hepatic gluconeogenesis. Lipocalin deficiency is commonly associated with obesity and is highly correlated with insulin resistance, mitochondrial dysfunction and dyslipidemia. 2003 saw the identification of two lipocalin receptors, Adipo R1 and Adipo R2.3, with Adipo R1 being the primary lipocalin receptor expressed in skeletal muscle, which is the primary glucose-utilizing tissue in the body.
Lipocalin signaling promotes activation of PGC1 alpha and mitochondrial biogenesis. Lipocalin binding to Adipo R1 leads to calcium inward flow in skeletal muscle, which activates CaMKK β. Activated CaMKK β induces the expression and activation of PGC1 α through a cascade reaction that involves the activation of AMPK and increased production of SIRT1 deacetylase. AMPK phosphorylation and SIRT1 deacetylation of PGC1 α increase its transcriptional activity, leading to increased mitochondrial biogenesis.
Lipocalin, an adipokine secreted by white adipose tissue, plays an important role in regulating glucose and lipid metabolism in insulin-sensitive tissues and in controlling energy homeostasis. APPL1 is the first identified protein that interacts directly with the lipocalin receptor.
We provide Luminex technology for adiponectin signaling pathway analysis.
Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. The core is the use of fluorescent dyes for encoding. By adjusting the different ratios of different fluorescent dyes, more than 100 microspheres with different fluorescence spectra were obtained. The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence.
Creative Proteomics has developed a signaling pathway target detection platform. We can provide detection services not only for adiponectin signaling pathways, but also for other signaling pathways. If you want to detect other targets, please contact us in advance and we will customize the service for you according to your requirements. Looking forward to working with you.