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What is Mouse Chemokine?
Mouse chemokine refers to a group of small signaling proteins that play a crucial role in the immune response by directing the movement of circulating leukocytes to sites of inflammation, infection, or trauma in mice. These proteins are a subset of cytokines and are characterized by their ability to induce chemotaxis in nearby responsive cells. Chemokines are classified into four main subfamilies based on the positioning of their conserved cysteine residues: CXC, CC, CX3C, and XC. Each subfamily exhibits distinct functional properties and roles in various physiological and pathological processes.
Chemokines are inextricably linked to the development and function of the immune system, influencing processes such as immune cell trafficking, lymphoid tissue organogenesis and host defense mechanisms. Analysis of these chemokines can provide insight into the molecular mechanisms of immune responses and help identify potential therapeutic targets for immune-related diseases.
Because of the physiological and genetic similarities between humans and mice, studies of mouse chemokines are often a precursor to understanding human chemokines. By studying these chemokines in mice, researchers can better understand their role in human health and disease, thereby facilitating the development of treatments for diseases such as autoimmune disorders, cancer and infectious diseases.
The study of mouse chemokines may help provide new insights into the function of the immune system as well as diagnostic and therapeutic aspects.
Mouse Chemokine Panel at Creative Proteomics
At Creative Proteomics, we offer comprehensive Mouse Chemokine Panel services utilizing advanced Luminex xMAP technology. Our panels enable the simultaneous quantification of multiple chemokines in a variety of biological samples, including serum, plasma, cell culture supernatants, and more. With our mouse chemokine panels, researchers can analyze key chemokine biomarkers involved in immune responses, inflammation, and disease progression. The panels are available in different configurations, including 9-plex and 16-plex, and are designed to deliver high sensitivity and reproducibility for a wide range of applications in immunology, oncology, and other areas of biomedical research. By utilizing a small sample volume, our assays provide efficient, high-throughput analysis, helping you accelerate your research and obtain reliable data for your studies.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Mouse | Mouse Chemokine 9-plex Panel | GROα/KC/CXCL1, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2, RANTES, Eotaxin | Suitable for studying immune responses, inflammation, and chemokine signaling pathways in mice. | +Inquiry |
| Mouse | Mouse Chemokine 16-plex Panel | CCL2/JE/MCP-1, CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL7/MCP-3/MARC, CCL8/MCP-2, CCL19/MIP-3 beta, CCL20/MIP-3 alpha, CCL21/6Ckine, CCL22/MDC, CXCL1/GRO alpha/KC/CINC-1, CXCL2/GRO beta/MIP-2/CINC-3, CXCL10/IP-10/CRG-2, CXCL13/BLC/BCA-1, CXCL16, IL-16, CCL11/Eotaxin | Ideal for comprehensive profiling of mouse chemokines in oncology, immunology, and inflammatory research. | +Inquiry |
Sensitivity (Detection Limits)
- GROα/KC/CXCL1: 1.0–10.0 pg/mL
- IP-10: 0.5–8.0 pg/mL
- MCP-1: 0.5–6.0 pg/mL
- MCP-3: 1.0–7.0 pg/mL
- MIP-1α: 1.0–8.0 pg/mL
- MIP-1β: 0.5–6.0 pg/mL
- MIP-2: 0.5–6.0 pg/mL
- RANTES: 1.0–8.0 pg/mL
- Eotaxin: 0.5–5.0 pg/mL
- CCL2/JE/MCP-1: 0.5–6.0 pg/mL
- CCL3/MIP-1α: 1.0–8.0 pg/mL
- CCL4/MIP-1β: 0.5–6.0 pg/mL
- CCL7/MCP-3/MARC: 1.0–7.0 pg/mL
- CCL8/MCP-2: 0.5–6.0 pg/mL
- CCL19/MIP-3β: 1.0–8.0 pg/mL
- CCL20/MIP-3α: 1.0–7.0 pg/mL
- CCL21/6Ckine: 1.0–8.0 pg/mL
- CCL22/MDC: 0.5–6.0 pg/mL
- CXCL1/GROα/KC/CINC-1: 1.0–7.0 pg/mL
- CXCL2/GROβ/MIP-2/CINC-3: 1.0–7.0 pg/mL
- CXCL10/IP-10/CRG-2: 0.5–6.0 pg/mL
- CXCL13/BLC/BCA-1: 0.5–6.0 pg/mL
- CXCL16: 1.0–8.0 pg/mL
- IL-16: 0.5–6.0 pg/mL
- CCL11/Eotaxin: 0.5–6.0 pg/mL
Advantages of the Mouse Chemokine Luminex Assay
- Multiplex Capability: The assay allows for the simultaneous detection of multiple chemokines in a single sample. With panels ranging from 9-plex to 31-plex, this multiplexing ability enables high-throughput analysis, reducing sample volume requirements and saving valuable research time.
- High Sensitivity and Specificity: The Luminex technology provides excellent sensitivity with detection limits as low as 0.5 pg/mL for certain chemokines, ensuring accurate quantification even for low-abundance biomarkers. The use of specific capture and detection antibodies enhances the specificity of the assay, minimizing cross-reactivity.
- Small Sample Volume Requirements: The assay requires as little as 25-50 µL of plasma, serum, or cell culture supernatant, making it ideal for precious or limited samples. This feature is particularly valuable in studies with small or difficult-to-collect sample types.
- Wide Range of Chemokines: The mouse chemokine Luminex panel covers a broad spectrum of chemokines involved in inflammation, immune response, and disease pathology, including targets like GROα, IP-10, MCP-1, RANTES, and more. This enables researchers to profile key chemokine networks in various biological contexts.
- Fast and Efficient: The assay offers rapid results, typically requiring only 4 hours from sample preparation to data acquisition. This speed makes it suitable for high-throughput screening and large-scale studies.
- Validated and Reproducible: The assay is extensively validated for reproducibility and accuracy across different experimental conditions. The reagents undergo rigorous testing for analyte combinability, interference, and cross-reactivity, ensuring consistent performance in a variety of experimental setups.
- Compatible with Luminex Platforms: The assay is fully compatible with Luminex xMAP technology platforms (e.g., Luminex 200, FLEXMAP 3D, MAGPIX), which are widely used in the research community for protein detection and quantification. This compatibility allows for easy integration into existing laboratory workflows.
- Versatile Applications: The mouse chemokine Luminex assay can be applied in various research fields, including immunology, oncology, neurology, and infectious diseases. It is particularly valuable for studying inflammatory responses, immune cell recruitment, and biomarker discovery.
Sample Requirements for Mouse Chemokine Luminex Panel
| Sample Type | Minimum Volume Required | Optimal Storage Conditions | Remarks |
|---|---|---|---|
| Serum | 25 µL | Store at -80°C | Avoid repeated freeze-thaw cycles |
| Plasma (EDTA, Heparin, Citrate) | 25 µL | Store at -80°C | Ensure no hemolysis and proper collection |
| Cell Culture Supernatants | 50 µL | Store at -80°C | Free of particulates and debris |
| Whole Blood | Inquire | Store at -80°C after separation | Requires additional processing |
| Urine | 50 µL | Store at -80°C | Filtered to remove debris |
| Cerebrospinal Fluid (CSF) | 50 µL | Store at -80°C | Ensure sterility |
| Bronchoalveolar Lavage Fluid | 50 µL | Store at -80°C | Collected under sterile conditions |
| Synovial Fluid | 50 µL | Store at -80°C | Pre-clarified if necessary |
| Other Biological Fluids | Inquire | Consult with technical experts | Suitability subject to prior testing |
Application of Mouse Chemokine Panel
- Immunology Research
- Analyze the role of chemokines in immune cell recruitment and inflammation.
- Study cytokine-chemokine interplay in immune responses and autoimmune diseases.
- Oncology
- Investigate tumor microenvironment dynamics and immune cell infiltration.
- Explore the potential of chemokines as biomarkers for cancer progression.
- Infectious Diseases
- Elucidate host-pathogen interactions through chemokine profiling.
- Characterize immune responses in viral, bacterial, or parasitic infections.
- Neurological Disorders
- Explore chemokine involvement in neuroinflammation and neurodegenerative diseases.
- Identify biomarkers for multiple sclerosis, Alzheimer's, and Parkinson's disease.
- Therapeutic Development
- Screen chemokine-related biomarkers for drug discovery.
- Assess the impact of therapeutic interventions on chemokine expression profiles.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| GROα/KC/CXCL1 | A chemokine that plays a crucial role in immune cell recruitment and inflammation, particularly in neutrophil activation and migration during acute inflammatory responses. |
| IP-10 (CXCL10) | A chemokine involved in immune responses by attracting T cells and natural killer cells to sites of infection or inflammation, often elevated in viral infections. |
| MCP-1 (CCL2) | A chemokine that mediates the recruitment of monocytes and macrophages to sites of inflammation, playing a significant role in chronic inflammatory diseases. |
| MCP-3 (CCL7) | A chemokine that recruits monocytes and other immune cells to inflammation sites, contributing to conditions like atherosclerosis and asthma. |
| MIP-1α (CCL3) | A pro-inflammatory chemokine that attracts a variety of immune cells, including T cells, monocytes, and dendritic cells, to inflammatory sites. |
| MIP-1β (CCL4) | Similar to MIP-1α, this chemokine attracts immune cells and is involved in inflammatory responses and immune regulation, often implicated in autoimmune diseases. |
| MIP-2 (CXCL2) | A neutrophil attractant that plays a role in the inflammatory response, especially in bacterial infections and tissue injury. |
| RANTES (CCL5) | A chemokine that recruits T cells, eosinophils, and monocytes to sites of inflammation, particularly in allergic reactions and autoimmune diseases. |
| Eotaxin (CCL11) | A chemokine primarily involved in recruiting eosinophils to sites of allergic inflammation, playing a central role in asthma and other allergic diseases. |
| GROβ (CXCL2) | A chemokine involved in the recruitment of neutrophils to sites of infection or injury, playing a significant role in the acute inflammatory response. |
| IP-10 (CXCL10) | Known for its role in the recruitment of T cells and natural killer cells during immune responses, especially in viral infections and autoimmune diseases. |
| MIP-3α (CCL20) | A chemokine that attracts T cells and dendritic cells to sites of inflammation, contributing to the immune response during infection and autoimmune diseases. |
| MIP-3β (CCL19) | A chemokine that regulates the migration of T cells, dendritic cells, and other immune cells, particularly involved in lymphoid tissue trafficking. |
| Fractalkine (CX3CL1) | A unique chemokine that has both soluble and membrane-bound forms, playing a role in immune cell adhesion and migration, particularly in neuroinflammation. |
| I-TAC (CXCL11) | A chemokine that attracts T cells and natural killer cells, often involved in the immune response to infections and in the regulation of chronic inflammation. |
| SCYB16 (CXCL16) | A dual-function chemokine that acts as a scavenger receptor ligand for monocytes and T cells, contributing to the regulation of immune responses and inflammation. |
| GM-CSF | A cytokine that stimulates the production and activation of immune cells, particularly granulocytes and macrophages, playing a key role in inflammation and immune responses. |
| KC (CXCL1) | A chemokine involved in the recruitment of neutrophils to inflammation sites, important in acute inflammation, especially in bacterial infections. |
| SDF-1α (CXCL12) | A chemokine that regulates hematopoiesis, immune cell trafficking, and tissue repair, playing a role in both acute and chronic inflammation. |
| I-309 (CCL1) | A chemokine that attracts T cells and monocytes to inflammation sites, contributing to immune responses in autoimmune diseases and chronic inflammation. |
| MCP-5 (CCL12) | A chemokine involved in monocyte and macrophage recruitment, important in inflammatory conditions like atherosclerosis and rheumatoid arthritis. |
| MDC (CCL22) | A chemokine that specifically attracts Th2 cells, contributing to allergic reactions and autoimmune diseases by modulating the immune response. |
| TNF-α | A major pro-inflammatory cytokine that mediates immune responses, cell death, and tissue damage, playing a central role in systemic inflammation and diseases like rheumatoid arthritis. |
| IFN-γ | A critical cytokine in immune responses, especially in viral infections and tumor surveillance, and involved in the regulation of macrophage and T cell function. |
| IL-1β | A potent cytokine that drives the inflammatory process by inducing fever, pain, and the activation of immune cells in response to infection and injury. |
| IL-2 | A cytokine crucial for T cell proliferation and activation, essential for adaptive immune responses and immune system homeostasis. |
| IL-4 | A cytokine that promotes Th2 cell differentiation and is involved in immune responses to allergens and parasitic infections, regulating antibody production and immune tolerance. |
| IL-6 | A pleiotropic cytokine that regulates immune responses, inflammation, and acute-phase protein production, often elevated in chronic inflammatory and autoimmune diseases. |
Can the Mouse Chemokine Panel detect chemokines from a wide range of tissues, such as brain or lung?
Yes, the Mouse Chemokine Panel is versatile and can be used to detect chemokines from a variety of tissue types, including blood, lung, liver, spleen, and brain. However, the effectiveness of chemokine detection depends on the abundance of the target analytes in those tissues. For example, chemokines that are predominantly expressed in the brain or lungs might be present in lower concentrations in blood or plasma, requiring more sensitive or optimized methods for detection. We recommend consulting with our technical team for guidance on tissue-specific applications and preparation protocols.
Are custom chemokine panels available for specific research needs?
Yes, Creative Proteomics offers customized Mouse Chemokine Panels tailored to your specific research goals. If you need to analyze additional chemokines not included in the standard 9-plex or 16-plex panels, we can create custom panels that are aligned with your experimental requirements. Our team of experts can assist in selecting the appropriate chemokines and configuring the assay to ensure the most relevant and comprehensive analysis for your study.
How does the Mouse Chemokine Panel compare to other protein detection methods like ELISA or Western blot?
The Mouse Chemokine Panel using Luminex xMAP technology offers several advantages over traditional methods like ELISA or Western blot. First, Luminex allows for the simultaneous detection of multiple chemokines in a single sample, significantly increasing throughput and efficiency. This is particularly valuable when studying complex immune responses or inflammatory pathways that involve multiple biomarkers. Additionally, Luminex assays generally provide higher sensitivity and a broader dynamic range compared to ELISA. While Western blot can provide detailed information on protein expression, it is time-consuming and limited to one analyte at a time. The Mouse Chemokine Panel provides a high-throughput, cost-effective alternative for large-scale studies.
