- Services Overview
- Analytes Details
- FAQ
What is Mouse Osteoprotegerin?
Osteoprotegerin (OPG) is a critical glycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. It acts as a decoy receptor for receptor activator of nuclear factor kappa-B ligand (RANKL), inhibiting its interaction with the receptor RANK on osteoclast precursors. By blocking this pathway, OPG prevents osteoclast differentiation, activation, and survival, thus playing a central role in bone remodeling by reducing bone resorption. This mechanism highlights OPG's pivotal role in maintaining skeletal health and its potential therapeutic implications in bone-related disorders.
In murine models, mouse osteoprotegerin (mOPG) is an essential biomarker for studying bone metabolism and diseases such as osteoporosis, osteopetrosis, multiple myeloma, and Paget's disease. Beyond its role in bone biology, OPG is implicated in various physiological and pathological processes, including immune system regulation, vascular calcification, and cancer biology. Its dynamic regulation makes it a valuable target for understanding the interplay between the skeletal system and other organ systems.
The Mouse osteoprotegerin panel is a highly specialized tool designed for the precise quantification of OPG in mouse-derived samples, such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this panel is optimized for both basic research and preclinical studies. It enables researchers to investigate OPG's function and its alterations in response to different physiological conditions or experimental interventions.
Key Biological Functions of Mouse Osteoprotegerin
- Bone Metabolism Regulation: By modulating the RANK/RANKL signaling axis, OPG serves as a natural inhibitor of osteoclastogenesis, helping maintain the balance between bone formation and resorption.
- Vascular Health: OPG plays a role in preventing vascular calcification by interacting with RANKL expressed in vascular tissues.
- Immune Modulation: OPG's involvement in the immune system suggests its regulatory effects on inflammatory pathways, making it relevant in autoimmune and inflammatory diseases.
- Cancer Pathophysiology: OPG has been linked to tumor progression, especially in cancers that metastasize to bone, such as breast and prostate cancers, where it can alter the bone microenvironment.
Mouse Osteoprotegerin Panel at Creative Proteomics
Luminex xMAP technology is a powerful tool that utilizes multiplexing capabilities to simultaneously measure multiple analytes in a single sample. This is achieved through the use of color-coded microspheres, each with specific antibodies attached, which capture target molecules. By allowing the simultaneous analysis of various proteins, Luminex xMAP accelerates the data collection process, delivering comprehensive results quickly and accurately.
Creative Proteomics offers Mouse Osteoprotegerin Panel through Luminex xMAP technology, which provides researchers and scientists with insights into bone health, disease mechanisms, and the effects of various treatments on bone density and strength.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Mouse | Mouse Osteoprotegerin 7-plex Panel | Dkk-1, IL-1 beta/IL-1F2, Leptin/OB, Osteopontin/OPN, TNF-alpha, TRANCE/TNFSF11/RANK L, IL-6 | Suitable for studying bone metabolism, inflammation, energy homeostasis, and immune regulation. | +Inquiry |
Sensitivity (Detection Limits)
- Dkk-1: 1.0–10.0 pg/mL
- IL-1 beta/IL-1F2: 0.5–5.0 pg/mL
- Leptin/OB: 2.0–15.0 pg/mL
- Osteopontin/OPN: 2.0–12.0 pg/mL
- TNF-alpha: 0.5–6.0 pg/mL
- TRANCE/TNFSF11/RANKL: 1.0–8.0 pg/mL
- IL-6: 0.5–7.0 pg/mL
Advantages of the Mouse Osteoprotegerin Luminex Assay
- High Sensitivity and Broad Detection Range: Accurately quantifies low-abundance proteins with detection limits ranging from sub-picogram to nanogram levels.
- Multiplexing Capability: Simultaneously measures multiple analytes (up to 7 in this panel), saving valuable sample volume and reducing experimental variability.
- Minimal Sample Requirement: Requires as little as 25–50 µL of serum, plasma, or other biological fluids, making it suitable for limited or precious sample types.
- Rapid Turnaround Time: Streamlined workflows supported by automated systems provide quick results, accelerating research timelines.
- Exceptional Reproducibility and Accuracy: Leveraging the bead-based Luminex xMAP technology ensures consistent and reliable data across replicates and experiments.
- Customizable Panel Options: Add or substitute additional biomarkers to tailor the assay for your specific research objectives.
- Wide Application Scope:Ideal for diverse studies, including bone metabolism, inflammatory diseases, immune response, and preclinical drug development.
Sample Requirements for Mouse Osteoprotegerin Luminex Panel
| Sample Type | Required Volume | Collection Method | Storage/Handling Instructions |
|---|---|---|---|
| Serum | 50 µL | Store at -80°C | Avoid repeated freeze-thaw cycles. |
| Plasma | 50 µL | Store at -80°C | Use EDTA or citrate as anticoagulants. |
| Cell Culture Supernatant | 200 µL | Store at -80°C | Filter to remove debris before submission. |
| Tissue Homogenate | 200 µL | Store at -80°C | Consult our experts for buffer recommendations. |
Application of Mouse Osteoprotegerin Panel
- Bone Metabolism Research
Osteoprotegerin (OPG) plays a pivotal role in bone remodeling by regulating osteoclast activity. The panel is ideal for:
- Investigating mechanisms of bone diseases like osteoporosis, osteopetrosis, and Paget's disease.
- Evaluating bone turnover markers in preclinical models of bone metastases.
- Understanding the interactions between RANKL, OPG, and other bone-specific factors.
- Inflammation and Immune Regulation
With key inflammatory cytokines like IL-6, TNF-alpha, and IL-1 beta included, the panel is suitable for:
- Studying chronic inflammatory conditions such as arthritis and autoimmune diseases.
- Exploring cytokine interactions and their impact on bone-immune system crosstalk (osteoimmunology).
- Cardiovascular Research
OPG and associated cytokines are linked to vascular calcification and atherosclerosis. The panel supports:
- Elucidating the role of OPG in vascular health and arterial stiffness.
- Assessing therapeutic interventions targeting vascular inflammation and calcification.
- Energy Homeostasis and Metabolic Studies
With the inclusion of leptin, a hormone critical for metabolism and bone-fat interactions, the panel is useful for:
- Exploring the interplay between metabolic regulation and bone health.
- Investigating obesity-related effects on bone integrity and systemic inflammation.
- Oncology Research
Bone is a common site for metastases in cancers such as breast and prostate cancer. The panel aids in:
- Profiling bone microenvironment changes during tumor progression.
- Studying the RANK/RANKL/OPG axis in bone metastases models.
- Drug Discovery and Preclinical Development
- Monitoring drug effects on bone turnover and immune modulation.
- Validating drug candidates targeting the RANKL-OPG pathway.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| Dkk-1 | A Wnt signaling inhibitor that regulates bone formation and remodeling, frequently studied in conditions of bone loss and inflammation. |
| IL-1 beta/IL-1F2 | A potent pro-inflammatory cytokine that plays a key role in initiating the immune response and mediating fever, pain, and inflammation. |
| Leptin/OB | A hormone involved in energy regulation and appetite control, also influencing bone metabolism and immune system activity during systemic inflammation. |
| Osteopontin/OPN | A multifunctional glycoprotein critical for bone remodeling, immune cell recruitment, and inflammatory response regulation. |
| TNF-alpha | A major inflammatory cytokine that orchestrates immune responses and apoptosis, contributing to systemic inflammation and bone resorption in disease states. |
| TRANCE/TNFSF11/RANKL | A key regulator of osteoclast differentiation and activation, pivotal in bone resorption and skeletal homeostasis. |
| IL-6 | A pleiotropic cytokine with roles in inflammation, immune response, and the acute-phase reaction, often elevated in inflammatory and bone-related diseases. |
How can the presence of interfering substances in the samples affect the results of the Mouse Osteoprotegerin Panel?
Interfering substances, such as hemoglobin in blood samples, high lipid concentrations, or cytokine inhibitors in serum/plasma, can affect the accuracy of the Mouse Osteoprotegerin Panel. Hemolysis, for example, can release additional proteins and hemoglobin, which can bind to the beads in the assay and result in false signals. Additionally, the presence of high levels of non-specific proteins could cause matrix effects, leading to inaccurate quantification. We recommend carefully handling and processing samples to minimize such interference and suggest using fresh, non-hemolyzed samples whenever possible for optimal results.
What are the consequences of repeated freeze-thaw cycles on my samples in the Mouse Osteoprotegerin Panel?
Repeated freeze-thaw cycles can degrade certain proteins, especially cytokines, and alter the integrity of the sample. This could result in inconsistent or inaccurate results. Proteins like osteoprotegerin and IL-6 are particularly sensitive to temperature fluctuations, which may lead to partial denaturation or aggregation. To ensure the accuracy of your results, we advise that samples be aliquoted into smaller volumes upon first thaw and stored at -80°C for long-term storage, avoiding any further freeze-thaw cycles.
Can I use the Mouse Osteoprotegerin Panel to quantify osteoprotegerin (OPG) levels in disease models with altered protein expression?
Yes, the Mouse Osteoprotegerin Panel is specifically designed to quantify osteoprotegerin (OPG) and several other related biomarkers involved in bone metabolism, immune regulation, and inflammatory processes. In disease models, especially those involving altered protein expression (such as in osteopetrosis or osteoporosis), this panel will provide valuable insights into changes in osteoclast activity and bone resorption. However, it is important to consider that certain conditions or experimental treatments may affect the biological activity of OPG or other cytokines, potentially altering their baseline levels. In such cases, we recommend using additional controls or normalization strategies in the experimental design.
