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What Are Mouse Kidney Biomarkers?
Mouse kidney biomarkers are substances used as indicators of biological processes or diseases within the kidneys of mice. These biomarkers help researchers understand kidney functions, detect potential issues, and monitor treatment effects. Biomarkers can include molecules like proteins, metabolites, or even specific genes associated with kidney function or disease.
Mouse models are widely used in biomedical research due to their genetic similarities to humans and their adaptability to experimental procedures. Understanding kidney biomarkers in mice contributes significantly to preclinical studies, enabling scientists to draw parallels to human kidney conditions. Through these studies, researchers can improve our knowledge of kidney diseases, such as acute kidney injury or chronic kidney disease, and develop new treatments.
Types of Kidney Biomarkers
Biomarkers can be classified into different types based on their role or origin:
- Functional Biomarkers: These indicate how well the kidney is functioning. For example, blood urea nitrogen (BUN) levels and creatinine are common measures of kidney filtration efficiency.
- Structural Biomarkers: These reflect changes in kidney tissues. Proteins like albumin in urine can signal damage to kidney structures.
- Molecular Biomarkers: These include genes or proteins that might change in expression due to kidney stress or damage. Examples include KIM-1 (Kidney Injury Molecule-1) and NGAL (Neutrophil Gelatinase-Associated Lipocalin).
Mouse Kidney Biomarker Panel at Creative Proteomics
At Creative Proteomics, we utilize Luminex xMAP technology to provide unparalleled accuracy and efficiency in Mouse Kidney Biomarker 19-plex Panel analysis. This cutting-edge multiplexing platform allows for the simultaneous detection and quantification of multiple kidney biomarkers in a single sample, ensuring robust and reproducible results tailored to your research needs.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Mouse | Mouse Kidney Biomarker 19-plex Panel | Adiponectin/Acrp30, CCL2/JE/MCP-1, CXCL1/GRO alpha/KC/CINC-1, CXCL10/IP-10/CRG-2, CXCL16, Cystatin C, EGF, IL-6, IL-10, MMP-9, Osteopontin/OPN, RAGE/AGER, Resistin, TIM-1/KIM-1/HAVCR, TNF-alpha, TNF RI/TNFRSF1A, TWEAK/TNFSF12, uPAR, DPPIV/CD26 | Suitable for analyzing kidney function, inflammation, nephrotoxicity, tissue remodeling, and disease biomarker discovery. | +Inquiry |
Sensitivity (Detection Limits)
- Adiponectin/Acrp30: 0.01–10 ng/mL
- CCL2/JE/MCP-1: 0.05–50 ng/mL
- CXCL1/GRO alpha/KC/CINC-1: 0.04–40 ng/mL
- CXCL10/IP-10/CRG-2: 0.01–10 ng/mL
- CXCL16: 0.005–5 ng/mL
- Cystatin C: 0.05–50 ng/mL
- EGF: 0.005–5 ng/mL
- IL-6: 0.01–10 ng/mL
- IL-10: 0.005–5 ng/mL
- MMP-9: 0.1–10 ng/mL
- Osteopontin/OPN: 0.01–10 ng/mL
- RAGE/AGER: 0.05–50 ng/mL
- Resistin: 0.005–5 ng/mL
- TIM-1/KIM-1/HAVCR: 0.2–250 ng/mL
- TNF-alpha: 0.01–10 ng/mL
- TNF RI/TNFRSF1A: 0.05–50 ng/mL
- TWEAK/TNFSF12: 0.1–100 ng/mL
- uPAR: 0.01–10 ng/mL
- DPPIV/CD26: 0.005–5 ng/mL
Advantages of the Mouse Kidney Biomarker Luminex Assay
- High Multiplexing Capacity: Analyze up to 50 biomarkers in a single run, conserving sample volume and reducing processing time.
- Fluorometric Detection: Delivers high sensitivity and specificity, with a detection range as low as 0.005 ng/mL.
- Broad Sample Compatibility: Suitable for a variety of sample types, including serum, plasma, urine, and tissue lysates.
- Reproducibility and Consistency: Optimized protocols ensure high data quality and minimal variation between runs.
- Time-Efficient Workflow: The multiplexing capability significantly reduces assay time compared to traditional single-analyte methods.
- Cost-Effective Solution: A single assay setup minimizes reagent use, lowering overall experimental costs.
- Customizable Options: Tailor the biomarker panel to include specific analytes relevant to your study.
Sample Requirements for Mouse Kidney Biomarker Luminex Panel
| Sample Type | Recommended Volume | Storage Conditions | Additional Notes |
|---|---|---|---|
| Serum | ≥ 50 µL | Store at -80°C | Collect samples in serum separator tubes, centrifuge to separate serum, and avoid hemolysis. |
| Plasma | ≥ 50 µL | Store at -80°C | Use EDTA or heparin-treated tubes; ensure proper mixing to prevent clotting before centrifugation. |
| Urine | ≥ 1 mL | Store at -80°C | Centrifuge to remove debris and store aliquots to minimize freeze-thaw cycles. |
| Tissue Lysate | ≥ 200 µg protein | Store at -80°C | Use appropriate lysis buffer with protease inhibitors; ensure complete homogenization. |
| Cell Culture Supernatant | ≥ 500 µL | Store at -80°C | Collect at appropriate cell density; centrifuge to remove cell debris before storage. |
| Whole Blood | ≥ 200 µL | Store at -80°C (as plasma or serum) | Immediately process for plasma or serum separation to avoid coagulation or degradation. |
| CSF (Cerebrospinal Fluid) | ≥ 200 µL | Store at -80°C | Centrifuge to remove particulate matter; aliquot to prevent freeze-thaw cycles. |
| Bronchoalveolar Lavage Fluid (BALF) | ≥ 500 µL | Store at -80°C | Filter to remove particulates; ensure samples are clear and free of contamination. |
Application of Mouse Kidney Biomarker Panel
- Renal Disease Mechanism Studies
Explore the molecular and cellular pathways underlying acute kidney injury (AKI), chronic kidney disease (CKD), and other renal pathologies.
- Toxicology and Pharmacology
Evaluate the nephrotoxic effects of drugs, environmental toxins, and chemicals, aiding in preclinical safety assessments.
- Biomarker Discovery
Identify and validate potential biomarkers for monitoring kidney injury, inflammation, and tissue remodeling.
- Immune Response Investigations
Assess inflammatory and anti-inflammatory cytokine profiles associated with kidney health and disease progression.
- Tissue Remodeling and Fibrosis Research
Study the dynamics of extracellular matrix proteins and regulators involved in renal tissue repair and scarring.
- Translational Kidney Research
Bridge basic research and therapeutic development by evaluating the molecular changes in experimental models.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| Adiponectin/Acrp30 | A hormone involved in glucose regulation and fatty acid breakdown; linked to inflammation and renal function. |
| CCL2/JE/MCP-1 | A chemokine critical for monocyte recruitment and inflammation, commonly elevated in kidney injury and immune responses. |
| CXCL1/GRO alpha/KC/CINC-1 | A chemokine involved in neutrophil recruitment and inflammatory pathways in renal diseases. |
| CXCL10/IP-10/CRG-2 | A pro-inflammatory chemokine that mediates immune cell trafficking during kidney injury and inflammation. |
| CXCL16 | Functions as a scavenger receptor and chemokine, with roles in immune response and kidney fibrosis. |
| Cystatin C | A sensitive marker of glomerular filtration rate, used to assess kidney function and injury. |
| EGF (Epidermal Growth Factor) | A growth factor involved in cell repair and proliferation; significant in renal recovery and regeneration. |
| IL-6 | A pro-inflammatory cytokine associated with inflammation, tissue injury, and kidney disease progression. |
| IL-10 | An anti-inflammatory cytokine that helps regulate immune responses and reduce tissue damage during kidney injury. |
| MMP-9 (Matrix Metalloproteinase-9) | A protease involved in extracellular matrix remodeling and tissue repair, often dysregulated in renal diseases. |
| Osteopontin (OPN) | A multifunctional protein that plays a role in fibrosis, immune response, and extracellular matrix regulation in kidney pathology. |
| RAGE/AGER | A receptor for advanced glycation end-products, contributing to oxidative stress and inflammation in kidney diseases. |
| Resistin | A hormone linked to inflammation and metabolic regulation, implicated in renal and cardiovascular diseases. |
| TIM-1/KIM-1/HAVCR | A key biomarker for detecting acute kidney injury and tubular damage. |
| TNF-alpha | A pro-inflammatory cytokine involved in immune regulation and kidney injury mechanisms. |
| TNF RI/TNFRSF1A | A receptor for TNF-alpha that mediates inflammation and cell death in renal pathophysiology. |
| TWEAK/TNFSF12 | A cytokine that promotes inflammation and fibrosis, associated with renal damage and progression to chronic kidney disease. |
| uPAR | A receptor involved in cell signaling, immune response, and tissue remodeling, often linked to glomerular diseases. |
| DPPIV/CD26 | An enzyme with roles in immune regulation and glucose metabolism, implicated in kidney injury and repair mechanisms. |
How do I ensure the integrity of my samples during collection and shipping?
- Collect samples in sterile, low-binding tubes to prevent protein loss.
- Use proper anticoagulants (e.g., EDTA or heparin for plasma) to avoid degradation.
- Snap-freeze samples in liquid nitrogen immediately after collection and store at -80°C.
- Ship samples on dry ice in insulated containers to prevent thawing during transit.
Avoid multiple freeze-thaw cycles as they can degrade sensitive biomarkers like cytokines or growth factors.
Can I analyze longitudinal samples from the same subject?
Yes, longitudinal sample analysis is highly recommended for understanding dynamic changes in kidney biomarkers over time. For such studies:
- Ensure consistent sample collection methods and timing (e.g., same time of day to account for diurnal variations).
- Use identical storage and handling procedures to minimize variability.
This approach is particularly valuable for drug efficacy studies or disease progression monitoring.
How reproducible are the results across multiple runs or batches?
The Luminex xMAP platform offers high reproducibility due to standardized bead-based detection and stringent quality control measures. Variability is typically <10% across runs. To further enhance reproducibility:
- Use aliquots from the same sample preparation batch for all replicates.
- Follow consistent pipetting and assay protocols.
For large studies spanning multiple batches, we provide calibration and reference samples to account for inter-batch variation.
Are there pre-assay quality checks for samples?
We offer pre-assay quality assessments, including:
- Protein concentration measurement (for tissue lysates and supernatants).
- Creatinine normalization (for urine samples).
- Hemolysis detection (for serum/plasma samples).
These checks ensure sample integrity and help troubleshoot issues before running the full panel, saving time and resources
Can I receive raw data and processed results?
Yes, we provide comprehensive data packages, including:
- Raw fluorescence intensity (FI) values.
- Processed data with standard curve fitting for biomarker quantification.
- Statistical analysis and visualizations upon request.
Our team can also guide you in interpreting the results or integrating them into downstream analyses.
