- Services Overview
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What are Human Myokines?
Human myokines are a diverse group of cytokines, growth factors, and other signaling molecules that are secreted by skeletal muscle cells during muscle contractions. These molecules act as critical mediators in various physiological processes, influencing metabolism, immune responses, and inter-organ communication. Myokines such as IL-6, Irisin, and Myostatin are key examples, each playing distinct roles in the regulation of muscle mass, energy expenditure, and inflammation.
Human myokines can function in an autocrine, paracrine, or endocrine manner, impacting not only muscle tissue but also distant organs such as the liver, adipose tissue, and brain. The myokine hypothesis suggests that these molecules are central to the health benefits of physical activity, offering molecular insights into the mechanisms by which exercise influences systemic metabolism, immune function, and even neuroplasticity.
Analyzing human myokines is crucial for understanding their role in health and disease. This analysis can reveal how exercise modulates myokine levels, offering insights into the prevention and treatment of metabolic disorders, cardiovascular diseases, and muscle-related conditions. Additionally, myokine profiling can help identify biomarkers for various diseases, enabling early diagnosis and the development of targeted therapies.
Human myokine analysis also provides valuable data for exercise physiology research, elucidating the molecular underpinnings of physical activity's effects on the body. By measuring changes in myokine levels in response to different exercise regimens, researchers can optimize training programs and develop personalized exercise interventions that maximize health benefits.
Human Myokine Panel at Creative Proteomics
Creative Proteomics leverages the power of Luminex xMAP technology to offer comprehensive myokine analysis services. The Human Myokine 15-plex Panel, utilizing magnetic bead-based multiplex assays, allows for the simultaneous quantification of 15 myokines in human serum, plasma, and cell or tissue supernatants. This multiplex approach provides a holistic view of the myokine landscape, enabling researchers to uncover complex biological interactions and gain deeper insights into disease mechanisms.
The Luminex xMAP platform integrates the principles of flow cytometry with advanced bead-based multiplexing, allowing for the detection of multiple analytes from minimal sample volumes. This technology is particularly valuable for studying myokine profiles in various contexts, from basic research to clinical applications.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Human
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Human | Human Myokine 15-plex Panel | Apelin, IL-15, Follistatin-like Protein 1 (FSTL1), Leukemia Inhibitory Factor (LIF), Oncostatin M (OSM), Fractalkine (CX3CL1), Osteocrin/Musclin, Myostatin/GDF8, Irisin, Osteonectin (SPARC), Erythropoietin (EPO), Brain-Derived Neurotrophic Factor (BDNF), Fatty Acid Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF-21), Interleukin-6 (IL-6) | Ideal for studying muscle physiology, metabolic regulation, and inflammation | +Inquiry |
Advantages of the Human Myokine Luminex Assay
- Multiplexing Capability: The ability to analyze up to 15 myokines simultaneously increases efficiency and reduces the amount of sample required, making it ideal for studies with limited sample availability.
- High Sensitivity and Precision: The assay provides highly sensitive and precise measurements, with intra-assay and inter-assay coefficients of variation (CVs) generally below 15%. This ensures reliable detection of even low-abundance myokines.
- Customizability: The assay can be tailored to include specific analytes of interest, allowing researchers to focus on the most relevant myokines for their study.
- Optimized Performance: The assay is designed with an optimized serum matrix to mimic native analyte environments, ensuring that the detection conditions are as close to physiological as possible.
- Lot-to-Lot Consistency: Rigorous quality control measures are in place to ensure batch-to-batch reproducibility, providing researchers with consistent and trustworthy results.
Sample Requirements for Human Myokine Luminex Panel
| Sample Type | Volume Required | Storage Conditions | Notes |
|---|---|---|---|
| Serum | 50-100 µL | 2 - 8°C | Avoid repeated freeze-thaw cycles |
| Plasma (EDTA or Heparin) | 50-100 µL | 2 - 8°C | Centrifuge within 30 minutes of collection |
| Cell Culture Supernatant | 100-200 µL | 2 - 8°C | Filtered and debris-free |
| Tissue Homogenate | 200 µL | 2 - 8°C | Homogenized in appropriate buffer |
Application of Human Myokine Panel
Exercise Physiology and Muscle Adaptation
The Human Myokine 15-plex Panel is a key tool for studying molecular responses to exercise. By analyzing myokine level changes, researchers gain insights into muscle adaptation, recovery, and growth, helping to optimize training regimens and improve athletic performance.
Metabolic Health Research
Myokines regulate metabolism, making their analysis critical for understanding conditions like obesity and insulin resistance. The panel allows researchers to link myokine profiles with metabolic health, guiding interventions for disease prevention.
Inter-Organ Communication
Myokines act as endocrine signals that influence other organs. The Human Myokine Panel helps explore muscle-to-organ communication, shedding light on systemic health effects of exercise and potential disease mechanisms.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Targets Details |
|---|
| Apelin: A peptide that regulates cardiovascular function, fluid balance, and energy metabolism. |
| Interleukin-15 (IL-15): A cytokine essential for the proliferation and function of NK and CD8+ T cells. |
| Follistatin-Like Protein 1 (FSTL1): A glycoprotein involved in inflammation, fibrosis, and tissue protection. |
| Leukemia Inhibitory Factor (LIF): A cytokine that regulates cell differentiation, survival, and inflammatory responses. |
| Oncostatin M (OSM): A cytokine involved in inflammation, bone formation, and tissue remodeling. |
| Fractalkine (CX3CL1): A chemokine that mediates leukocyte adhesion and migration during inflammation. |
| Osteocrin/Musclin: A peptide hormone that influences bone growth and muscle function. |
| Myostatin/GDF8: A negative regulator of muscle growth and differentiation. |
| Irisin: A hormone that promotes the browning of adipose tissue and enhances energy expenditure. |
| Osteonectin (SPARC): A matricellular protein involved in tissue remodeling and cellular responses. |
| Erythropoietin (EPO): A hormone that stimulates red blood cell production in response to low oxygen levels. |
| Brain-Derived Neurotrophic Factor (BDNF): A protein that supports neuron growth, survival, and synaptic plasticity. |
| Fatty Acid Binding Protein 3 (FABP3): A protein that binds and transports fatty acids within cells, affecting metabolic processes. |
| Fibroblast Growth Factor 21 (FGF-21): A hormone that regulates glucose and lipid metabolism and is involved in energy homeostasis. |
| Interleukin-6 (IL-6): A cytokine that modulates immune responses, inflammation, and hematopoiesis. |
How does the Human Myokine Panel handle the potential cross-reactivity between different myokines in the multiplex assay?
The Human Myokine Panel is designed with stringent validation protocols to minimize cross-reactivity between different myokines in the multiplex assay. Each bead set in the Luminex xMAP technology is coated with highly specific capture antibodies that bind only to their target myokine. Additionally, the assay undergoes rigorous testing to ensure that the detection antibodies used do not cross-react with other analytes in the panel. This ensures that the quantification of each myokine is accurate and reliable, even when multiple myokines are measured simultaneously.
What quality control measures are in place to ensure the consistency and reliability of myokine measurements?
Creative Proteomics implements comprehensive quality control measures at multiple stages of the assay process. Each batch of reagents is tested for performance consistency, and standard curves are run with every assay to ensure accuracy. The Luminex platform is also calibrated regularly to maintain precision in bead-based measurements. Furthermore, intra-assay and inter-assay variability are carefully monitored, with coefficients of variation (CVs) typically maintained below 15%. These quality control protocols ensure that myokine measurements are both consistent and reliable across different sample sets and experimental conditions.
How are the data from the Human Myokine Panel typically interpreted, and do you provide support for data analysis?
Data interpretation from the Human Myokine Panel involves understanding the complex interactions between the various myokines measured. Creative Proteomics provides detailed data output, including concentration values for each myokine in the sample. We also offer data analysis support to help researchers interpret their results in the context of their specific study. This can include statistical analysis, data visualization, and insights into the biological significance of observed myokine profiles. Our team is available to assist with these aspects to ensure that you can make the most of the data generated from your myokine analysis.
How does the Human Myokine Panel address variability in myokine levels due to different physiological or environmental factors?
Variability in myokine levels can be influenced by numerous factors, including age, sex, diet, physical activity, and underlying health conditions. The Human Myokine Panel is designed to account for such variability by providing highly sensitive and specific measurements. Creative Proteomics also recommends normalizing myokine data to account for individual differences and using appropriate controls to distinguish between biological variability and experimental noise. Additionally, our experts can help you design your study to minimize the impact of confounding factors and ensure robust, reproducible results.
What are the typical turnaround times for results from the Human Myokine Panel analysis?
The typical turnaround time for results from the Human Myokine Panel analysis is approximately 2 to 3 weeks from the receipt of samples. This timeline includes sample processing, assay setup, data acquisition, and quality control checks. However, turnaround times can vary depending on the number of samples and the complexity of the analysis. If you have specific time constraints, Creative Proteomics offers expedited services, and our team can work with you to meet critical deadlines.