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Protease Inhibitor in Mouse
Protease inhibitors are critical molecules that regulate the activity of proteases, enzymes responsible for breaking down proteins into smaller peptides or amino acids. In mouse models, protease inhibitors play essential roles in maintaining physiological balance and preventing excessive proteolytic activity that could otherwise lead to pathological conditions. These inhibitors are involved in diverse biological processes, such as immune system modulation, tissue remodeling, blood coagulation, and inflammation resolution. For instance, protease inhibitors like alpha-1-antitrypsin and tissue inhibitors of metalloproteinases (TIMPs) are key to controlling inflammation and tissue repair by inhibiting serine proteases and metalloproteases, respectively. Dysregulation of protease inhibitor levels or function in mouse models often mirrors disease states observed in humans, such as chronic inflammatory diseases, cancer progression, and neurodegenerative disorders. By studying protease inhibitors in mice, researchers can gain valuable insights into their mechanistic roles and identify potential therapeutic interventions. Mouse models are particularly advantageous due to their genetic and physiological similarities to humans, enabling the study of protease inhibitors in controlled, reproducible experimental conditions. This research is instrumental in unraveling the molecular underpinnings of diseases and accelerating the development of targeted treatments that modulate protease inhibitor pathways.
Mouse Protease Inhibitor Panel at Creative Proteomics
Creative Proteomics offers Mouse Protease Inhibitor Panel powered by Luminex xMAP technology. This platform allows for the simultaneous detection and quantification of key protease inhibitors with high sensitivity and specificity. Designed to meet the needs of protease regulation research, this service provides a reliable and efficient solution for exploring biological processes such as inflammation and extracellular matrix remodeling, as well as pathological mechanisms such as cancer progression and fibrosis.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Application | Price |
|---|---|---|---|---|
| Mouse | Mouse Protease Inhibitor 4-plex Panel | Cystatin C, Serpin E1/PAI-1, TIMP-1, EMMPRIN/CD147 | Suitable for studying protease regulation, matrix turnover, inflammatory response, and tumor microenvironment. | +Inquiry |
Sensitivity (Detection Limits)
- Cystatin C: 5–10 pg/mL
- Serpin E1/PAI-1: 6–12 pg/mL
- TIMP-1: 8–15 pg/mL
- EMMPRIN/CD147: 10–18 pg/mL
Advantages of Mouse Protease Inhibitor Luminex Assay
- High Sensitivity and Specificity: Detects low-abundance protease inhibitors with exceptional accuracy, ensuring reliable and reproducible results.
- Multiplex Capability: Simultaneous analysis of multiple targets in a single assay, conserving time and sample volume.
- Wide Dynamic Range: Precisely quantifies both high- and low-concentration analytes in the same run.
- Minimal Sample Volume: Requires as little as 50 µL, ideal for limited or precious samples.
- Cost-Effective: Reduces reagent and labor costs by consolidating multiple tests into one assay.
- Customizable Panels: Tailored to include specific protease inhibitors based on your research needs.
Sample Requirements for Mouse Protease Inhibitor Luminex Panel
| Sample Type | Volume Required | Notes |
|---|---|---|
| Serum | 50 µL | Collect using standard serum separation protocols; avoid hemolysis. |
| Plasma (EDTA/Citrate) | 50 µL | Use anticoagulants like EDTA or citrate; ensure complete mixing. |
| Cell Culture Supernatant | 100 µL | Filter to remove debris; ensure samples are free from contaminants. |
| Tissue Lysate | 100 µg total protein | Homogenize in appropriate lysis buffer; ensure consistent protein yield. |
| Whole Blood | 100 µL | Use appropriate anticoagulants; process promptly to avoid degradation. |
| Urine | 200 µL | Collect midstream; centrifuge to remove cells and debris. |
| Saliva | 200 µL | Ensure collection is done under non-contaminated conditions. |
Application of Mouse Protease Inhibitor Panel
- Inflammation and Immune Response: Study the role of protease inhibitors in inflammatory diseases, autoimmune disorders, and immune system modulation.
- Cancer Research: Investigate how protease inhibitors influence tumor progression, metastasis, and the tumor microenvironment.
- Tissue Remodeling and Fibrosis: Analyze protease inhibitors in fibrosis-related diseases, including pulmonary, liver, and kidney fibrosis.
- Neurodegenerative Diseases: Explore protease inhibitor dynamics in neurodegenerative conditions such as Alzheimer's and Parkinson's disease.
- Cardiovascular Disease: Examine the role of protease inhibitors in cardiovascular health, including tissue repair and blood coagulation.
- Infection and Pathogen Defense: Investigate the protective functions of protease inhibitors against bacterial and viral infections.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| Cystatin C | A potent inhibitor of cysteine proteases, involved in regulating extracellular matrix turnover and immune responses. Dysregulation is linked to neurodegenerative diseases, cardiovascular disorders, and cancer progression. |
| Serpin E1/PAI-1 (Plasminogen Activator Inhibitor-1) | Inhibits plasminogen activators like tPA and uPA, crucial for controlling fibrinolysis. Elevated levels are associated with thrombosis, cancer metastasis, and metabolic disorders. |
| TIMP-1 (Tissue Inhibitor of Metalloproteinases-1) | A key inhibitor of matrix metalloproteinases (MMPs), involved in regulating tissue remodeling and inflammation. Imbalances in TIMP-1 are implicated in fibrosis, arthritis, and tumorigenesis. |
| EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) | Modulates MMP activity and is involved in tumor progression, angiogenesis, and immune regulation. Its expression is linked to cancer invasion, inflammation, and tissue remodeling. |
Can the Mouse Protease Inhibitor Panel be used with frozen samples?
Yes, the panel can be used with frozen samples. However, it is essential to ensure that samples are thawed properly and handled carefully to prevent degradation or changes in protease inhibitor levels. For best results, avoid repeated freeze-thaw cycles and process samples as soon as possible after thawing.
Is the Mouse Protease Inhibitor Panel suitable for high-throughput screening?
Yes, the Mouse Protease Inhibitor Panel is well-suited for high-throughput screening of protease inhibitor levels across multiple samples. Its multiplex capability allows for simultaneous detection of several analytes in one assay, making it ideal for screening large numbers of samples in a cost- and time-efficient manner. This is especially beneficial for studies involving animal models or clinical trials.
Can I perform follow-up studies using the same samples?
Once a sample has been processed and analyzed, it cannot be used for follow-up studies using the same protocol due to the limited volume requirements and potential degradation of analytes during the initial analysis. However, if additional tests are needed, we recommend submitting new or freshly prepared samples to ensure accurate results.
What is the role of the multiplex assay in the Mouse Protease Inhibitor Panel?
The multiplex capability of the Luminex xMAP technology allows for the simultaneous quantification of multiple protease inhibitors in a single sample, which significantly reduces sample volume requirements and testing time. This method provides highly efficient and cost-effective analysis while ensuring comprehensive data on the regulation of protease inhibitors in biological systems.
