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Mouse Immune Monitoring
Immune monitoring plays a critical role in understanding the immune system's function in disease progression and therapeutic response. In preclinical research, mouse models are widely used due to their genetic similarities to humans and their well-characterized immune system. Studying immune cell populations, cytokine profiles, and immune activation pathways in these models provides essential insights for drug discovery, vaccine development, and immunotherapy evaluation.
Monitoring the immune response in mouse models is often complex and requires precise, multi-parameter analysis. Traditional methods, such as ELISA or flow cytometry, provide valuable data but can be limited in throughput and scope, especially when analyzing numerous cytokines or immune mediators simultaneously. To overcome these challenges, multiplexing technologies like the Luminex platform have become indispensable for high-throughput and comprehensive immune profiling.
The mouse immune monitoring panel enables simultaneous measurement of multiple cytokines, chemokines, and other immune biomarkers in a single, small-volume sample. By leveraging bead-based multiplexing, the system provides robust, reproducible, and quantitative data. This capability is especially advantageous for preclinical studies where sample volumes are limited and multi-analyte analysis is required to evaluate complex immune dynamics.
Mouse Immune Monitoring Panel at Creative Proteomics
Creative Proteomics's Mouse Immune Monitoring Panel leverages Luminex xMAP technology to provide a comprehensive solution for analyzing immune responses in mouse models. This high-throughput assay enables the simultaneous quantification of up to 48 cytokines, chemokines, and growth factors, offering in-depth insights into immune regulation, inflammation, and disease mechanisms. Designed with exceptional sensitivity and specificity, the panel supports various sample types, ensuring broad applicability. With robust performance and reliable data output, the mouse immune monitoring panel is an essential tool for preclinical research, biomarker discovery, and therapeutic development.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Mouse | Mouse Immune Monitoring 48-plex Panel | BAFF, G-CSF (CSF-3), GM-CSF, IFN alpha, IFN gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15/IL-15R, IL-17A (CTLA-8), IL-18, IL-19, IL-22, IL-23, IL-25 (IL-17E), IL-27, IL-28, IL-31, IL-33, LIF, M-CSF, RANKL, TNF alpha, ENA-78 (CXCL5), Eotaxin (CCL11), GRO alpha (CXCL1), IP-10 (CXCL10), MCP-1 (CCL2), MCP-3 (CCL7), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-2, RANTES (CCL5), Betacellulin (BTC), Leptin, VEGF-A, Betacellulin (BTC), Leptin, VEGF-A | Suitable for analyzing immune responses, cytokine and chemokine networks, and inflammatory processes in preclinical mouse models. | +Inquiry |
Sensitivity (Detection Limits)
- BAFF: 1.0–10.0 pg/mL
- G-CSF (CSF-3): 0.5–6.0 pg/mL
- GM-CSF: 1.0–8.0 pg/mL
- IFN-α: 0.5–5.0 pg/mL
- IFN-γ: 0.5–8.0 pg/mL
- IL-1α: 0.5–7.0 pg/mL
- IL-1β: 0.5–6.0 pg/mL
- IL-2: 0.5–8.0 pg/mL
- IL-3: 1.0–9.0 pg/mL
- IL-4: 0.5–6.0 pg/mL
- IL-5: 1.0–8.0 pg/mL
- IL-6: 0.5–5.0 pg/mL
- IL-7: 0.5–6.0 pg/mL
- IL-9: 0.5–7.0 pg/mL
- IL-10: 0.5–8.0 pg/mL
- IL-12p70: 1.0–9.0 pg/mL
- IL-13: 0.5–7.0 pg/mL
- IL-15/IL-15R: 0.5–6.0 pg/mL
- IL-17A (CTLA-8): 0.5–7.0 pg/mL
- IL-18: 1.0–10.0 pg/mL
- IL-19: 0.5–6.0 pg/mL
- IL-22: 1.0–9.0 pg/mL
- IL-23: 0.5–8.0 pg/mL
- IL-25 (IL-17E): 1.0–10.0 pg/mL
- IL-27: 0.5–6.0 pg/mL
- IL-28: 0.5–7.0 pg/mL
- IL-31: 1.0–10.0 pg/mL
- IL-33: 1.0–8.0 pg/mL
- Betacellulin (BTC): 0.5–7.0 pg/mL
- Leptin: 1.0–9.0 pg/mL
- VEGF-A: 0.5–8.0 pg/mL
- LIF: 0.5–7.0 pg/mL
- M-CSF: 0.5–8.0 pg/mL
- RANKL: 0.5–7.0 pg/mL
- TNF-α: 0.5–6.0 pg/mL
- ENA-78 (CXCL5): 1.0–10.0 pg/mL
- Eotaxin (CCL11): 0.5–6.0 pg/mL
- GRO-α (CXCL1): 1.0–10.0 pg/mL
- IP-10 (CXCL10): 0.5–8.0 pg/mL
- MCP-1 (CCL2): 0.5–6.0 pg/mL
- MCP-3 (CCL7): 1.0–7.0 pg/mL
- MIP-1α (CCL3): 1.0–8.0 pg/mL
- MIP-1β (CCL4): 0.5–6.0 pg/mL
- MIP-2: 0.5–6.0 pg/mL
- RANTES (CCL5): 1.0–8.0 pg/mL
Advantages of Mouse Immune Monitoring Luminex Assay
- High Multiplexing Capability: Analyze multiple immune biomarkers in a single assay, reducing sample requirements and streamlining workflows.
- Exceptional Sensitivity and Specificity: Detect low-abundance cytokines and chemokines with high accuracy, ensuring reliable insights even in challenging samples.
- Small Sample Volume: Requires as little as 25–50 µL of sample per analysis, ideal for preclinical studies with limited material availability.
- Rapid Turnaround Time: Our optimized workflows and expert team ensure timely delivery of high-quality data to meet project deadlines.
- Customizable Panels: Choose from pre-designed panels or work with our team to create a custom solution tailored to your research objectives.
- Comprehensive Data Analysis: Receive detailed and well-organized reports with advanced statistical analysis, enabling actionable insights into immune responses.
Sample Requirements for Mouse Immune Monitoring Luminex Panel
| Sample Type | Minimum Volume | Collection & Preparation | Storage Conditions | Notes |
|---|---|---|---|---|
| Serum | 25–50 µL | Collect using a clot activator tube; allow clotting at room temperature, then centrifuge to isolate the serum. | Store at -80°C | Avoid hemolysis and multiple freeze-thaw cycles. |
| Plasma | 25–50 µL | Collect using EDTA or heparin tubes; centrifuge immediately after collection. | Store at -80°C | Ensure proper anticoagulant is used based on the research goal. |
| Cell Culture Supernatant | 25–50 µL | Harvest supernatant after appropriate stimulation or incubation, centrifuge to remove debris. | Store at -20°C or -80°C | Filter samples to ensure removal of particulate matter; avoid contamination. |
| Tissue Homogenates | 50 µL | Homogenize tissue in appropriate buffer with protease inhibitors; centrifuge to collect the supernatant. | Store at -80°C | Ensure thorough homogenization and avoid multiple freeze-thaw cycles. |
| Bronchoalveolar Lavage (BAL) | 25–50 µL | Centrifuge collected lavage fluid to remove debris or cells; collect supernatant for analysis. | Store at -80°C | Ensure samples are free from particulate contaminants before freezing. |
| Cerebrospinal Fluid (CSF) | 25–50 µL | Collect aseptically; centrifuge to remove any debris if necessary. | Store at -80°C | Due to limited sample volume, ensure minimal handling loss. |
Application of Mouse Immune Monitoring Panel
- Preclinical Research: Evaluate the efficacy and safety of therapeutic candidates, such as vaccines, immunotherapies, and anti-inflammatory drugs, in mouse models.
- Inflammation and Autoimmune Disease Studies:Analyze cytokine and chemokine profiles to understand the mechanisms of inflammation, autoimmune disorders, and immune dysregulation.
- Cancer Immunology: Investigate tumor microenvironments and immune response modulation, including immune checkpoint activity and tumor-associated cytokine production.
- Infectious Disease Research: Monitor host immune responses to infections, including viral, bacterial, and parasitic pathogens, for understanding disease progression and immune protection.
- Biomarker Discovery: Identify and validate immune-related biomarkers for disease diagnosis, prognosis, and therapeutic monitoring.
- Translational Medicine: Bridge preclinical findings to clinical applications by understanding immune mechanisms in mouse models that mimic human conditions.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| BAFF | A cytokine involved in B cell survival and differentiation; crucial for autoimmune disease development and immune responses. |
| G-CSF (CSF-3) | A growth factor that stimulates the production of neutrophils; plays a role in inflammation, tissue injury, and immune response regulation. |
| GM-CSF | A cytokine involved in the stimulation of granulocytes and macrophages; regulates immune cell production and inflammation. |
| IFN alpha | A type I interferon that plays a significant role in antiviral immune responses and regulating inflammation. |
| IFN gamma | A key cytokine in immune responses against infection; essential for Th1 cell differentiation and promoting macrophage activation. |
| IL-1 alpha | An inflammatory cytokine that is involved in immune responses, tissue repair, and the development of diseases such as arthritis and cancer. |
| IL-1 beta | A potent pro-inflammatory cytokine involved in immune responses, fever, and a key factor in autoimmune diseases such as rheumatoid arthritis. |
| IL-2 | A cytokine critical for T cell proliferation and activation; plays a role in immune tolerance and immunity in autoimmune diseases. |
| IL-3 | A cytokine that supports the growth and differentiation of hematopoietic cells; important for immune responses and hematopoiesis. |
| IL-4 | A cytokine promoting Th2 responses, involved in allergic responses, and key in regulating immune homeostasis. |
| IL-5 | Plays a major role in the activation and growth of eosinophils; important in the development of allergic diseases. |
| IL-6 | A cytokine with pro-inflammatory properties; involved in immune response regulation and the development of chronic inflammation. |
| IL-7 | Essential for T cell development and survival; contributes to immune system regulation and homeostasis. |
| IL-9 | A cytokine involved in allergic responses and Th2 immunity; supports the survival and differentiation of immune cells. |
| IL-10 | An anti-inflammatory cytokine that suppresses immune responses; plays a role in regulating autoimmune diseases and inflammation. |
| IL-12p70 | A cytokine that influences Th1 immune responses and plays a role in autoimmune diseases and defense against intracellular pathogens. |
| IL-13 | A cytokine that regulates allergic responses, immune tolerance, and the development of diseases like asthma and fibrosis. |
| IL-15/IL-15R | Plays a role in the proliferation and activation of natural killer cells and T cells; important for immune defense and inflammation regulation. |
| IL-17A (CTLA-8) | A cytokine involved in the recruitment of neutrophils and macrophages during inflammation; implicated in autoimmune diseases like psoriasis. |
| IL-18 | A pro-inflammatory cytokine involved in immune activation, particularly in response to infections and the development of autoimmune diseases. |
| IL-19 | A cytokine that contributes to inflammation and immune regulation; associated with chronic inflammatory diseases. |
| IL-22 | Plays a role in inflammation and the protection of epithelial cells during immune responses; involved in autoimmune diseases and tissue repair. |
| IL-23 | A cytokine critical for the expansion of Th17 cells, involved in inflammation and autoimmune conditions such as psoriasis and Crohn's disease. |
| IL-25 (IL-17E) | A cytokine that enhances Th2 immune responses and is implicated in allergic diseases and asthma. |
| IL-27 | Involved in regulating Th1 responses and anti-inflammatory pathways; has a role in controlling autoimmune diseases and infection resolution. |
| IL-28 | A type III interferon with antiviral properties, playing a role in immune regulation and inflammation control. |
| IL-31 | A cytokine involved in allergic responses and inflammation; plays a role in pruritus (itching) in conditions like eczema. |
| IL-33 | Involved in Th2 immune responses and inflammation, particularly in the context of asthma and other allergic conditions. |
| LIF | A growth factor that promotes tissue repair and cell survival; has neuroprotective and anti-inflammatory properties. |
| M-CSF | A growth factor involved in the differentiation of monocytes and macrophages; essential for immune responses and inflammation resolution. |
| RANKL | A key regulator of bone remodeling and osteoclast differentiation; involved in inflammation and bone-related diseases. |
| TNF alpha | A pro-inflammatory cytokine involved in immune response regulation and the pathogenesis of autoimmune diseases and infections. |
| ENA-78 (CXCL5) | A chemokine involved in neutrophil recruitment during inflammation; plays a role in respiratory and gastrointestinal diseases. |
| Eotaxin (CCL11) | A chemokine that recruits eosinophils, involved in allergic inflammation, asthma, and other eosinophilic diseases. |
| GRO alpha (CXCL1) | A chemokine involved in the recruitment of neutrophils; plays a role in inflammation, wound healing, and cancer progression. |
| IP-10 (CXCL10) | A chemokine involved in the recruitment of T cells and monocytes during inflammation; important in viral infections and autoimmune diseases. |
| MCP-1 (CCL2) | A chemokine that recruits monocytes and T cells during inflammation; associated with chronic inflammation and tissue damage in conditions like atherosclerosis. |
| MCP-3 (CCL7) | Involved in the recruitment of monocytes, macrophages, and T cells during immune responses; associated with inflammation and tissue injury. |
| MIP-1 alpha (CCL3) | A chemokine that promotes the recruitment of immune cells like monocytes and T cells; involved in inflammation and immune responses to infections. |
| MIP-1 beta (CCL4) | A cytokine that attracts monocytes, T cells, and eosinophils; involved in the immune response to pathogens and tissue damage. |
| MIP-2 | A chemokine that promotes neutrophil recruitment; plays a significant role in acute inflammation and infection response. |
| RANTES (CCL5) | A chemokine that recruits immune cells such as T cells, eosinophils, and basophils; involved in inflammation, HIV, and allergy-related conditions. |
| Betacellulin (BTC) | A growth factor involved in tissue repair and cell proliferation; plays a role in neuroprotection and tissue regeneration in injury and disease. |
| Leptin | A hormone and cytokine that regulates energy balance, immune function, and inflammation; linked to obesity, diabetes, and immune responses. |
| VEGF-A | A growth factor critical for angiogenesis and tissue repair; plays a role in inflammation and the development of tumors. |
Can I use the Mouse Immune Monitoring Panel for longitudinal studies?
Yes, the Mouse Immune Monitoring Panel is well-suited for longitudinal studies, where you need to track immune responses over time. The panel's high sensitivity and reproducibility ensure that you can detect changes in immune profiles across multiple time points, allowing you to monitor the effects of treatments or disease progression in mouse models.
How does the Mouse Immune Monitoring Panel compare to other immune profiling methods?
This panel offers several advantages over traditional methods like ELISA, Western blot, and flow cytometry. Unlike ELISA, which typically requires a separate test for each biomarker, the Luminex multiplex technology enables the simultaneous quantification of multiple cytokines, chemokines, and growth factors from a single small-volume sample. This reduces sample consumption, improves throughput, and offers a more comprehensive analysis in a single assay.
