- Services Overview
- Analytes Details
- FAQ
What Are Th9, Th17, and Th22 Cells?
T-helper (Th) cells are a subset of T lymphocytes that play pivotal roles in regulating immune responses and maintaining immune homeostasis.
Th9 cells are integral to allergic reactions and autoimmune diseases due to their production of interleukin-9 (IL-9). Their role in inflammatory responses and tissue repair makes them a key focus for research into allergies and autoimmune disorders. By analyzing Th9 cells, scientists can explore how these cells contribute to disease progression and identify novel therapeutic targets to mitigate their adverse effects.
Th17 cells are known for their production of interleukin-17 (IL-17), which is crucial for fighting off certain pathogens but also contributes to chronic inflammation and autoimmune conditions like rheumatoid arthritis and psoriasis. Studying Th17 cells helps to elucidate their dual role in both protecting against infections and driving inflammatory diseases. This understanding can lead to targeted therapies designed to balance Th17 activity, potentially alleviating symptoms and improving patient outcomes in these chronic conditions.
Th22 cells play a significant role in maintaining the integrity of epithelial tissues and responding to infections. Their involvement in inflammatory skin conditions and some cancers highlights the importance of investigating their functions. By examining Th22 cells, researchers can gain insights into their role in mucosal immunity and tissue repair, paving the way for new treatments for diseases like psoriasis and certain types of cancer.
Human Th9/Th17/Th22 Discover Panel at Creative Proteomics
Creative Proteomics employs the Human Th9/Th17/Th22 Discover 17-plex Panel using Luminex xMAP technology to analyze cytokines from Th9, Th17, and Th22 cells. This panel allows for the simultaneous measurement of 17 cytokines in a single assay, enhancing efficiency and accuracy. Luminex xMAP technology utilizes color-coded beads to capture and quantify multiple analytes, offering a comprehensive cytokine profile with high sensitivity and specificity. This approach streamlines the analysis process, providing detailed insights into immune responses and facilitating targeted research and clinical applications.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Human
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Human | Human Th9/Th17/Th22 Discover 17-plex Panel | CD40 Ligand, GM-CSF, IFN-gamma, lL-1beta, IL-2,1L-4,1L-5, IL-6,1L-10,1L-12p70,1L-13, IL-15,1L-17A,1L-17E,1L-33, MIP-3 alpha, TNF alpha | Suitable for detailed analysis of cytokine profiles related to Th9, Th17, and Th22 cells, aiding in the study of immune responses and related diseases. | +Inquiry |
Advantages of the Human Th9/Th17/Th22 Discover Luminex Assay
- Multiplex Capability: The assay measures 17 cytokines simultaneously, saving time and resources compared to individual assays.
- High Sensitivity and Specificity: It provides accurate and reliable cytokine measurements, crucial for precise immune profiling.
- Quantitative Results: The assay delivers quantitative data, allowing for detailed analysis of cytokine concentrations and immune responses.
- Efficiency: By combining multiple measurements in one run, it streamlines the workflow and reduces sample and reagent usage.
- Versatility: Suitable for a wide range of applications, from basic research to clinical studies, enhancing its utility in various settings.
Sample Requirements for Human Th9/Th17/Th22 Discover Luminex Panel
| Sample Type | Volume Required | Storage Conditions | Notes |
|---|---|---|---|
| Plasma | 100 µL | -80°C or below | 6 months |
| Serum | 100 µL | -80°C or below | 6 months |
| Cell Culture Supernatant | 200 µL | -80°C or below | 3 months |
| Peripheral Blood Mononuclear Cells (PBMCs) | 1 x 10^6 cells | -80°C or below | 3 months |
Collection: Use appropriate anticoagulants or collection tubes to prevent clotting.
Centrifugation: Spin blood samples at 1,500-2,000 x g for 10 minutes to separate plasma or serum.
Aliquoting: Store samples in aliquots to avoid repeated freeze-thaw cycles.
Storage: Maintain samples at -80°C or lower to preserve integrity.
Application of Human Th9/Th17/Th22 Cytokine Panel
Immunological Research:
T Helper Cell Profiling: Analyze the cytokine profile associated with Th9, Th17, and Th22 cells to understand their roles in immune responses and inflammatory diseases.
Mechanistic Studies: Investigate the mechanisms underlying immune responses, autoimmunity, and chronic inflammation by studying the cytokines produced by these T helper subsets.
Disease Research:
Autoimmune Diseases: Explore the involvement of Th9, Th17, and Th22 cells in autoimmune diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
Infectious Diseases: Study how these cytokines contribute to the immune response during infections or how their dysregulation might impact disease progression.
Biomarker Discovery:
Disease Markers: Identify potential biomarkers for disease diagnosis, prognosis, or therapeutic response by measuring cytokine levels associated with specific conditions.
Therapeutic Targets: Evaluate the effects of new drugs or treatments on cytokine production and Th cell activity.
Immunotherapy Development:
Therapeutic Monitoring: Monitor cytokine levels to assess the efficacy and safety of immunotherapies targeting Th cell subsets or cytokines.
Basic Immunology:
Cytokine Interaction: Study interactions between different cytokines and their impact on immune cell function and behavior.
Cell Differentiation: Investigate the differentiation pathways of Th9, Th17, and Th22 cells and their role in immune regulation.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Targets Details |
|---|
| CD40 Ligand: A key costimulatory molecule essential for T cell activation and B cell interaction, influencing immune responses and autoimmunity. |
| GM-CSF: Granulocyte-macrophage colony-stimulating factor, important for the differentiation and activation of hematopoietic cells, particularly in inflammation and infection. |
| IFN-gamma: Interferon-gamma, crucial for macrophage activation and enhancing cellular immunity, pivotal in defense against intracellular pathogens. |
| IL-1beta: Interleukin-1beta, a pro-inflammatory cytokine that plays a central role in mediating inflammatory responses and fever. |
| IL-2: Interleukin-2, essential for T cell proliferation, differentiation, and survival, and a key regulator of the adaptive immune response. |
| IL-4: Interleukin-4, a cytokine that promotes Th2 cell differentiation and is involved in allergic responses and inflammation. |
| IL-5: Interleukin-5, important for eosinophil differentiation and activation, implicated in allergic and inflammatory diseases. |
| IL-6: Interleukin-6, a cytokine with roles in inflammation, immune regulation, and hematopoiesis, often elevated in chronic inflammatory conditions. |
| IL-10: Interleukin-10, an anti-inflammatory cytokine that regulates immune responses, suppresses inflammation, and promotes tolerance. |
| IL-12p70: A heterodimeric cytokine critical for Th1 cell differentiation and enhancing cellular immunity against infections. |
| IL-13: Interleukin-13, involved in regulating Th2 responses and allergic inflammation, influencing tissue repair and fibrosis. |
| IL-15: Interleukin-15, important for the proliferation and maintenance of memory T cells and natural killer cells, contributing to immune memory. |
| IL-17A: Interleukin-17A, a pro-inflammatory cytokine associated with autoimmune diseases and chronic inflammation, driving tissue damage and inflammation. |
| IL-17E: Interleukin-17E, modulates Th2 and Th17 cell functions, implicated in allergic responses and chronic inflammation. |
| IL-33: Interleukin-33, an alarmin that activates innate and adaptive immune responses, linked to asthma and inflammatory diseases. |
| MIP-3 alpha: Macrophage inflammatory protein-3 alpha, a chemokine involved in the recruitment and activation of immune cells to inflammatory sites. |
| TNF alpha: Tumor necrosis factor-alpha, a key mediator of systemic inflammation and immune response, central to various inflammatory diseases. |
How do I analyze the data obtained from the assay?
To analyze data from the Human Th9/Th17/Th22 Cytokine Panel, start by using the Luminex xMAP Data Analysis Software. This software processes raw data and generates cytokine concentrations based on the standard curve provided with the panel. For accurate comparisons across different samples or experiments, normalize the data to control values or total protein concentration. For statistical analysis, use methods such as t-tests, ANOVA, or regression analysis, depending on your experimental design, to determine significant differences or correlations.
Can I use this panel for longitudinal studies or repeated measurements from the same samples?
Yes, the panel can be used for longitudinal studies or repeated measurements. Ensure that samples are stored at -80°C to maintain stability over time and thaw them only once before analysis to prevent degradation. For consistency in results, follow the same assay protocol for each time point.
How can I validate the assay results in my specific research context?
To validate assay results, perform replicate experiments to assess reproducibility and consistency. Compare the data obtained with results from alternative methods, such as ELISA or Western blot, if feasible. Additionally, review relevant literature to ensure that the cytokine levels and patterns observed align with known biological or pathological contexts.
What are the key considerations when scaling up the assay for high-throughput screening?
When scaling up for high-throughput screening, ensure compatibility with 96-well or 384-well plates. Consider using automated pipetting systems to handle large numbers of samples efficiently and maintain consistency. Utilize robust data analysis software to manage and analyze large datasets effectively.
Can I use this panel to analyze cytokine levels in different types of biological fluids, such as cerebrospinal fluid (CSF) or saliva?
The panel is validated for plasma, serum, and cell culture supernatants. For other biological fluids like CSF or saliva, you may need to validate the panel yourself. Conduct preliminary tests to ensure compatibility and be aware of potential matrix effects that could impact assay performance. Consider sample pre-treatment or dilution if necessary.
How should I interpret cytokine levels that fall below or above the standard curve range?
If cytokine levels are below the detection limit, try increasing the sample volume or concentrating the sample. Confirm low levels using alternative methods if needed. If levels exceed the standard curve range, dilute the samples and re-run the assay to bring the cytokine levels within the detectable range.
Can the panel be used for validation of new cytokine assays or multiplex panels?
Yes, the panel can be used to validate new assays or multiplex panels. Compare results obtained with established standards and controls to benchmark new assay performance, ensuring consistency and reliability.