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Mouse Kidney Injury
Mouse kidney injury is an important area of study, particularly because of its implications for understanding human kidney diseases. Mice are commonly used as models in biomedical research due to their genetic, biological, and behavioral similarities to humans, as well as the convenience of their care and breeding. The study of mouse kidney injury allows researchers to explore the mechanisms of kidney damage, assess potential therapeutic strategies, and improve understanding of various renal pathologies.
Kidney injuries in mice can be induced through a variety of methods, including ischemia-reperfusion injury, nephrotoxicity from drugs like cisplatin, and other chemical or surgical techniques that mimic diseases such as acute kidney injury (AKI) and chronic kidney disease (CKD). These models are instrumental in dissecting the cellular and molecular pathways involved in kidney damage and repair processes.
One significant advantage of mouse models is the ability to utilize genetically modified strains. This allows for the investigation of specific genes and their roles in kidney function and injury. For example, knockout mice can help identify the protective or harmful effects of certain genes during kidney stress.
Research on mouse kidney injury contributes to the discovery of potential biomarkers for early diagnosis and the development of new therapeutic agents. By understanding the underlying mechanisms of injury and recovery in mice, scientists gain insights that could translate into improved treatment options for human kidney diseases.
Creative Proteomics's mouse kidney injury panel provides a comprehensive solution for detecting and quantifying multiple biomarkers involved in inflammation, oxidative stress, cell death, and fibrosis associated with kidney injury.
Mouse Kidney Injury Panel at Creative Proteomics
The Luminex xMAP platform revolutionizes multiplexed biomarker analysis by enabling the simultaneous quantification of multiple analytes in a single sample. This efficiency, paired with high sensitivity, makes it an ideal choice for Mouse Kidney Injury Panel analysis.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Mouse | Mouse Kidney Injury 5-plex Panel | Clusterin, CystatinC, EGF, NGAL/Lipocalin-2, Osteopontin (OPN) | Suitable for analyzing renal function, injury responses, and biomarker discovery in kidney studies. | +Inquiry |
| Mouse | Mouse Kidney Injury 6-plex Panel | IP-10/CXCL10, KIM-1, Renin, TIMP-1, VEGF-A, β-2-Microglobulin | Ideal for studying inflammation, fibrosis, and nephrotoxicity in preclinical models. | +Inquiry |
Sensitivity (Detection Limits)
- Adiponectin/Acrp30: 0.01–10 ng/mL
- CCL2/JE/MCP-1: 0.05–50 ng/mL
- CXCL1/GRO alpha/KC/CINC-1: 0.04–40 ng/mL
- CXCL10/IP-10/CRG-2: 0.01–10 ng/mL
- CXCL16: 0.005–5 ng/mL
- Cystatin C: 0.05–50 ng/mL
- EGF: 0.005–5 ng/mL
- IL-6: 0.01–10 ng/mL
- IL-10: 0.005–5 ng/mL
- MMP-9: 0.1–10 ng/mL
- Osteopontin/OPN: 0.01–10 ng/mL
- RAGE/AGER: 0.05–50 ng/mL
- Resistin: 0.005–5 ng/mL
- TIM-1/KIM-1/HAVCR: 0.2–250 ng/mL
- TNF-alpha: 0.01–10 ng/mL
- TNF RI/TNFRSF1A: 0.05–50 ng/mL
- TWEAK/TNFSF12: 0.1–100 ng/mL
- uPAR: 0.01–10 ng/mL
- DPPIV/CD26: 0.005–5 ng/mL
Advantages of the Mouse Kidney Injury Luminex Assay
- Multiplexing Efficiency: Simultaneously detects multiple kidney injury biomarkers in a single sample, saving time and resources.
- High Sensitivity and Specificity: Provides accurate detection of low-abundance biomarkers with minimal cross-reactivity.
- Cost-Effective Solution: Reduces assay costs by consolidating multiple tests and minimizing sample volume requirements.
- Robust and Reproducible Results: Ensures consistent, high-quality data across replicates and batches.
- Flexible and Customizable Panels: Offers pre-designed or tailored panels to meet diverse research needs.
- Streamlined Workflow: Features high-throughput capabilities and quick turnaround times for efficient analysis.
- Broad Research Applications: Supports nephrotoxicity screening, biomarker discovery, therapeutic evaluation, and disease modeling.
Sample Requirements for Mouse Kidney Injury Luminex Panel
| Sample Type | Minimum Volume/Amount Required | Storage Conditions | Preparation Notes |
|---|---|---|---|
| Serum/Plasma | 50 µL | Store at -80°C | Collect in EDTA or heparin tubes; avoid hemolysis and repeated freeze-thaw cycles. |
| Urine | 200 µL | Store at -80°C | Centrifuge to remove debris prior to storage; ensure sterile collection. |
| Tissue Homogenates | 100 µg protein | Store at -80°C | Homogenize with appropriate lysis buffer containing protease inhibitors. |
| Cell Culture Supernatant | 200 µL | Store at -80°C | Filter or centrifuge to remove cell debris. |
Application of Mouse Kidney Injury Panel
- Nephrotoxicity Screening: Detects kidney injury caused by drugs, aiding preclinical safety evaluations.
- Biomarker Discovery: Identifies and validates novel biomarkers for early diagnosis and disease progression.
- Therapeutic Evaluation: Monitors the efficacy of nephroprotective drugs and regenerative therapies.
- Pathophysiological Research: Explores mechanisms like inflammation, fibrosis, and oxidative stress in kidney injury.
- Disease Modeling: Supports studies using mouse models of acute kidney injury (AKI) and chronic kidney disease (CKD).
- Regenerative Medicine: Assesses the impact of cell therapies and tissue engineering on kidney repair.
- Inflammatory and Fibrotic Pathways: Analyzes key markers involved in inflammation and fibrosis during kidney injury.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| Clusterin | A glycoprotein involved in cellular stress response, tissue remodeling, and apoptosis regulation, commonly elevated in kidney injury. |
| Cystatin C | A sensitive marker of glomerular filtration rate, widely used to assess kidney function and injury. |
| EGF (Epidermal Growth Factor) | A growth factor promoting cell repair and proliferation, critical for renal recovery and regeneration. |
| NGAL/Lipocalin-2 | A biomarker for early kidney injury, indicative of tubular damage and inflammation. |
| Osteopontin (OPN) | A multifunctional protein linked to inflammation, fibrosis, and immune regulation in kidney injury. |
| IP-10/CXCL10 | A pro-inflammatory chemokine mediating immune cell recruitment during kidney injury and inflammation. |
| KIM-1 | A highly specific marker for tubular injury, widely used in nephrotoxicity and kidney disease studies. |
| Renin | An enzyme critical in blood pressure regulation and renal perfusion, altered in kidney disorders. |
| TIMP-1 | A tissue inhibitor of metalloproteinases, associated with extracellular matrix remodeling and fibrosis in renal injury. |
| VEGF-A | A vascular growth factor involved in angiogenesis and endothelial function, significant in renal health and disease. |
| β-2-Microglobulin | A marker of tubular dysfunction and systemic inflammation, elevated in chronic and acute kidney injuries. |
Can I analyze a specific biomarker not included in the pre-designed panels?
Yes, we offer customizable panels. If your target biomarker is not part of our standard 5-plex or 6-plex panels, our team can design and validate a custom assay using the Luminex xMAP platform. Please contact us to discuss feasibility and additional timelines.
Can I use the panel for longitudinal studies?
Absolutely. The Mouse Kidney Injury Panel is ideal for longitudinal studies, as it requires minimal sample volumes and delivers consistent, reproducible results. This makes it suitable for tracking kidney injury biomarkers over time in preclinical models.
How do you handle sample variability across different types (e.g., serum vs. urine)?
Each sample type is processed using optimized protocols to ensure reliable biomarker detection. For example, urine samples are centrifuged to remove particulates, while serum and plasma are prepared to avoid clotting or hemolysis. Data normalization and careful calibration further account for inherent variability between sample types.
Can the panel differentiate between acute and chronic kidney injury?
Yes, the panel includes biomarkers that are specific to acute kidney injury (e.g., KIM-1, NGAL) and chronic kidney injury (e.g., TIMP-1, Osteopontin). By analyzing the expression profiles of these markers, researchers can differentiate between the two conditions and gain insights into the underlying pathology.