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What is Mouse Bone Metabolism?
Bone metabolism refers to the dynamic process through which new bone is formed by osteoblasts and old bone is resorbed by osteoclasts. This intricate balance is vital for maintaining bone health and plays a crucial role in supporting body structure, protecting organs, anchoring muscles, and storing calcium. In mice, as in other mammals, bone metabolism is influenced by numerous factors including hormones, cytokines, and mechanical stress.
In scientific research, understanding bone metabolism in mice provides valuable insights into human bone diseases such as osteoporosis and arthritis. Mice are often used as model organisms due to their genetic similarity to humans and the ability to manipulate their genes to study specific aspects of bone physiology.
Research techniques like the Luminex Panel allow for the multiplex analysis of bone metabolism markers, enabling the simultaneous measurement of multiple biomarkers in a single sample. This technique enhances the understanding of the molecular mechanisms regulating bone health and disease, potentially leading to the development of novel therapeutic strategies.
Mouse Bone Metabolism Panel at Creative Proteomics
The Mouse Bone Metabolism Panel at Creative Proteomics provides an advanced platform for analyzing critical biomarkers involved in bone health, resorption, and formation. Employing Luminex xMAP technology, this panel enables high-throughput, multiplexed analysis, making it a highly effective tool for researchers exploring bone metabolism in preclinical mouse models.
Our panels offer several customizable multiplex assays tailored to meet diverse research needs. You can choose between single-plex and various multi-plex panels based on the specific targets and applications relevant to their studies.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Applications | Price |
|---|---|---|---|---|
| Mouse | Mouse Single-Plex Panel | RANKL | Suitable for focused studies on RANKL and its role in bone resorption and osteoclastogenesis | +Inquiry |
| Mouse | Mouse Bone Metabolism 7-Plex Panel | Dkk-1, IL-1 beta/IL-1F2, Leptin/OB, Osteopontin/OPN, TNF-alpha, TRANCE/TNFSF11/RANK L, IL-6 | Enables comprehensive profiling of bone formation, resorption, and inflammation markers | +Inquiry |
| Mouse | Mouse Bone Metabolism 10-Plex Panel | ACTH, DKK-1, FGF-23, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteoprotegerin (OPG), Sclerostin (SOST), TNFα | Offers an extensive view of regulatory factors involved in bone density, remodeling, and metabolic interactions | +Inquiry |
Advantages of the Mouse Bone Metabolism Luminex Assay
- Multiplexing capability: The Luminex platform allows the simultaneous analysis of multiple bone metabolism markers from a single sample, accelerating data collection and supporting high-throughput studies.
- Enhanced sensitivity and specificity: Each bead in the Luminex assay is coated with specific capture antibodies, ensuring sensitive and precise detection of biomarkers, even at low concentrations—critical for monitoring subtle changes in bone metabolism.
- Low sample volume requirements: Minimal sample volume is needed for each multiplex assay, preserving valuable samples, especially important in animal studies where sample quantities are limited.
- Fast processing and data turnaround: The automated Luminex workflow shortens processing times, enabling researchers to receive data quickly and adjust experimental approaches in real time.
- High reproducibility: This panel has been rigorously validated to ensure reliable, consistent results across experiments, providing robust data for conclusive analysis.
- Customizable assays: The panel can be tailored to include specific protein targets, meeting the needs of various research areas, from bone disease studies to metabolic research. This flexibility enables detailed investigation into specific pathways and mechanisms in bone metabolism.
- Comprehensive biomarker coverage: This panel includes a wide range of bone metabolism markers, providing an in-depth view of bone health, remodeling, and disease processes—essential for understanding the complex factors in bone diseases and developing targeted therapeutic strategies.
Sample Requirements for Mouse Bone Metabolism Luminex Panel
| Sample Type | Volume Required | Collection Notes |
|---|---|---|
| Serum | 50-100 µL | Collect in serum separator tubes, centrifuge, and store at -80°C |
| Plasma (EDTA/heparin) | 50-100 µL | Collect in anticoagulant tubes, centrifuge, and store at -80°C |
| Bone Tissue Homogenate | 100-200 mg | Homogenize in buffer, centrifuge to remove debris, and store at -80°C |
| Cell Culture Supernatant | 200 µL | Collect from confluent cultures, centrifuge, and store at -80°C |
| Urine | 500 µL | Collect fresh, centrifuge to remove particulates, and store at -80°C |
| Synovial Fluid | 100 µL | Collect aseptically, centrifuge if necessary, and store at -80°C |
| Bone Marrow Aspirate | 100 µL | Collect aseptically, centrifuge to separate cells if needed, and store at -80°C |
| Whole Blood | 100 µL | Collect with anticoagulant, gently mix, and store at -80°C for cellular analysis |
Application of Mouse Bone Metabolism Panel
- Osteoporosis and Bone Density Studies
Allows researchers to monitor bone resorption and formation markers critical in osteoporosis.
- Bone-Related Disease Mechanisms
Ideal for identifying changes in bone metabolism in models of arthritis, metabolic disorders, and inflammatory conditions.
- Drug Development
Supports the evaluation of novel therapies targeting bone health and diseases, including the effects of drugs on bone remodeling and osteogenesis.
- Endocrine and Metabolic Studies
Enables exploration of hormonal impacts on bone metabolism, including insulin and leptin's effects on bone turnover and health.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| RANKL (Receptor Activator of Nuclear Factor κB Ligand) | Essential for osteoclast differentiation and bone resorption; critical in bone remodeling and osteoporosis research. |
| Dkk-1 (Dickkopf-1) | An antagonist of Wnt signaling pathway, involved in bone formation inhibition and bone density regulation. |
| IL-1 beta (Interleukin-1 beta) | Pro-inflammatory cytokine linked to bone resorption; plays a key role in inflammatory responses and bone metabolism. |
| Leptin/OB (Obesity Protein) | A hormone primarily associated with energy regulation, also involved in bone metabolism by impacting bone formation and resorption processes. |
| Osteopontin (OPN) | A multifunctional protein linked to bone remodeling, immune responses, and extracellular matrix regulation; associated with chronic inflammation. |
| TNF-alpha (Tumor Necrosis Factor-alpha) | A cytokine with a prominent role in inflammation, contributing to osteoclast activation and bone resorption in inflammatory bone diseases. |
| TRANCE/TNFSF11/RANKL | Also known as RANKL, essential for osteoclast maturation and bone resorption, critical for studies in osteoclastogenesis and bone loss conditions. |
| IL-6 (Interleukin-6) | Cytokine involved in immune responses and bone metabolism, influencing both osteoblast and osteoclast activity. |
| ACTH (Adrenocorticotropic Hormone) | Regulates glucocorticoid levels, impacting bone density; important in studies of metabolic bone diseases. |
| FGF-23 (Fibroblast Growth Factor-23) | A hormone regulating phosphate metabolism and vitamin D levels, essential for bone mineralization and kidney function. |
| Insulin | A hormone that impacts glucose metabolism and bone formation, influencing osteoblast activity and skeletal health. |
| Osteocalcin (OC) | A bone-specific protein produced by osteoblasts, important as a marker of bone formation and overall bone health. |
| Osteoprotegerin (OPG) | A decoy receptor for RANKL, inhibiting osteoclastogenesis and bone resorption, important in bone density studies. |
| Sclerostin (SOST) | A Wnt pathway antagonist secreted by osteocytes, regulating bone formation and remodeling, relevant in osteoporosis research. |
How does the sensitivity of the Mouse Bone Metabolism Panel compare with other assays for bone metabolism markers?
The Mouse Bone Metabolism Panel leverages Luminex xMAP technology, known for its high sensitivity and specificity, particularly suitable for detecting low-abundance biomarkers that traditional ELISA methods might miss. This technology can detect changes in analyte concentrations at sub-picogram levels for some targets, allowing precise quantification of biomarkers that are critical to studying bone metabolism and disease. Furthermore, multiplexing provides context across a range of markers in one assay, enhancing interpretability by detecting multiple analytes in a single sample.
What quality control measures are in place to ensure the reproducibility of results?
Our Mouse Bone Metabolism Panel service adheres to stringent quality control protocols, including the use of validated calibration standards and controls for each analyte. During each run, we incorporate technical replicates and control samples to ensure data consistency. Additionally, all assay reagents and consumables are lot-matched to minimize variability. These measures, coupled with Luminex xMAP's automated processing, help achieve consistent results across multiple experiments, allowing researchers to compare data confidently across time points or treatment groups.
What types of research are best suited for the Mouse Bone Metabolism Panel?
This panel is designed for a wide array of research applications, particularly preclinical studies in bone health, osteoporosis, rheumatoid arthritis, and metabolic bone disorders. Researchers studying the impact of therapeutic interventions on bone metabolism markers, as well as those investigating the role of specific signaling pathways (e.g., Wnt, RANK/RANKL/OPG axis), will find this panel especially useful. Its high sensitivity also makes it suitable for studies looking to track subtle changes over time or in response to experimental treatments.
What should I do if I have limited sample volume? Can the assay still be performed?
Yes, the Luminex xMAP platform is optimized for small-volume samples, making it feasible to perform assays with as little as 50-100 µL for most sample types. If you have a particularly limited sample volume, please discuss it with our technical team. We may be able to optimize sample handling and multiplexing configurations to maximize data yield from each sample.
