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Myokine in Mouse
Myokines are cytokines or peptides produced by skeletal muscle cells during contraction, playing key roles in regulating various physiological processes. Studies in mice have provided valuable insights into their mechanisms and implications for health and disease.
In mice, myokine secretion is triggered by muscle contraction and exercise. These molecules are synthesized by myocytes and released into the bloodstream, where they act on distant tissues such as the liver, adipose tissue, brain, and immune cells. Key signaling pathways like AMPK and mTOR regulate myokine production. Mouse models provide a controlled environment to study these mechanisms at the molecular level.
Myokines are pivotal in metabolic regulation. For instance, irisin promotes browning of white adipose tissue, increasing energy expenditure. Interleukin-6 (IL-6), released during exercise, enhances glucose uptake and fatty acid oxidation in skeletal muscles, improving metabolic health.
Mouse studies reveal the immunomodulatory roles of myokines. Myostatin suppresses pro-inflammatory cytokines, while IL-15 enhances natural killer (NK) cell function and maintains immune homeostasis. These findings highlight the interplay between muscle and the immune system mediated by myokines.
Mouse models show that myokines are involved in various diseases. Elevated myostatin is linked to muscle atrophy in conditions like cancer cachexia and aging. Conversely, exercise-induced myokines such as brain-derived neurotrophic factor (BDNF) exhibit neuroprotective effects, reducing cognitive decline in neurodegenerative diseases. These insights suggest therapeutic potential by modulating myokine activity.
Mouse models bridge basic research and clinical applications. By studying genetically modified mice or specific environmental conditions, researchers can target beneficial or harmful myokines to treat metabolic disorders, inflammation, and sarcopenia.
Mouse Myokine Panel at Creative Proteomics
Creative Proteomics offers the Mouse Myokine 5-plex Panel assay using Luminex xMAP technology. This platform utilizes fluorescently stained microspheres and flow cytometry to enable the simultaneous quantification of five key muscle cytokines in a single sample. By combining multiplexing capabilities with high sensitivity and specificity, our assay panels provide accurate and reliable data that are ideally suited for the study of muscle physiology, inter-organ communication, and related pathological processes.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Application | Price |
|---|---|---|---|---|
| Mouse | Mouse Myokine 5-plex Panel | IL-6, IL-10, IL-15/IL-15R, LIF, TNF alpha | Suitable for studying muscle physiology, inflammation, metabolism, and systemic signaling. | +Inquiry |
Sensitivity (Detection Limits)
- IL-6: 1–5 pg/mL
- IL-10: 2–6 pg/mL
- IL-15/IL-15R: 5–10 pg/mL
- LIF: 8–12 pg/mL
- TNF alpha: 3–7 pg/mL
Advantages of Mouse Myokine Luminex Assay
- Enhanced Multiplexing Power: The Luminex assay facilitates the detection of up to 50 myokines simultaneously in a single sample. This multiplexing capability ensures a holistic view of myokine profiles while conserving valuable samples.
- Unmatched Sensitivity and Accuracy: Utilizing fluorescently labeled microspheres, the Luminex platform achieves superior detection sensitivity, crucial for profiling low-abundance myokines. The precision of this assay guarantees reproducibility and confidence in data interpretation.
- Cost-Effective Solution: With the ability to analyze multiple analytes in a single reaction, this assay reduces reagent consumption, labor, and associated costs, providing exceptional value for researchers.
- Scalability and Flexibility: Whether you require a standard panel or a fully customized solution, the Luminex assay is adaptable to meet diverse project needs.
Sample Requirements for Mouse Myokine Luminex Panel
| Sample Type | Sample Volume | Storage Conditions |
|---|---|---|
| Serum | 50 µL | -80°C |
| Plasma (EDTA/Heparin) | 50 µL | -80°C |
| Tissue Homogenate (muscle, liver, etc.) | 100 µg protein | -80°C |
| Cell Culture Supernatant (from muscle cell lines or primary muscle cultures) | 100 µL | -80°C |
| Cytokine or Myokine Standards | 50 µL | -80°C |
Applications of Mouse Myokine Panel
- Metabolic Research: Elucidate the role of myokines in energy homeostasis, insulin sensitivity, and obesity-related mechanisms.
- Inflammation Studies: Explore the involvement of myokines in inflammatory signaling and their implications in autoimmune diseases.
- Cardiovascular Research: Investigate myokine-mediated inter-organ communication and its impact on cardiovascular health.
- Muscle Physiology and Aging: Analyze the alterations in myokine secretion during aging and muscle-related disorders.
- Drug Discovery and Therapeutics: Evaluate the therapeutic potential of targeting myokine pathways in disease intervention.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| IL-6 | A cytokine involved in immune response and inflammation; plays a crucial role in muscle metabolism, glucose homeostasis, and inflammatory signaling during exercise or injury. |
| IL-10 | An anti-inflammatory cytokine that regulates immune responses; involved in tissue repair and suppression of pro-inflammatory cytokines, playing a protective role in chronic inflammation and autoimmune diseases. |
| IL-15/IL-15R | A cytokine/receptor complex that promotes the activation and survival of natural killer (NK) cells and CD8+ T cells; crucial in immune homeostasis and muscle regeneration. |
| LIF | A cytokine that supports cell survival and differentiation; involved in neuroprotection, muscle regeneration, and the modulation of inflammatory responses during tissue injury. |
| TNF alpha | A pro-inflammatory cytokine that regulates immune responses and inflammation; plays a critical role in muscle wasting, systemic inflammation, and diseases like cachexia and rheumatoid arthritis. |
Can I use fresh samples for analysis?
While fresh samples can be used, we recommend freezing the samples at -80°C immediately after collection to maintain their stability. This prevents potential degradation of myokines and ensures the best possible data quality.
What is the turnaround time for the mouse myokine panel results?
The typical turnaround time for the mouse myokine panel is around 2-3 weeks, depending on sample volume and the complexity of the assay. Our team will provide an estimated timeline upon receiving your samples.
Can the mouse myokine panel be used to study disease models?
Absolutely. The mouse myokine panel is ideal for investigating disease models related to inflammation, muscle degeneration, metabolic disorders, and aging. It provides valuable insights into the role of myokines in various physiological and pathological conditions.
What is the minimum number of samples I need to submit?
While there is no strict minimum, we recommend submitting at least three biological replicates to ensure statistical reliability. However, if you have fewer samples, we can still proceed with the analysis and provide helpful guidance.
What kind of data analysis will I receive?
Once the analysis is complete, you will receive a detailed report that includes raw data, statistical analysis, and graphical representations of your results. Our team is available to assist in interpreting the data and discussing the implications of the findings.
Can the mouse myokine panel be used for longitudinal studies?
Yes, the Mouse Myokine Panel is suitable for longitudinal studies where multiple time points or treatment conditions are examined. We can accommodate your specific research needs and ensure consistent data collection across multiple sample sets.
